Microparticles: a component of various diseases

Budaj, Miroslav; Poljak, Zuzana; Duris, I; Imrich, Richard; Kopáni, Martin; Maruscakova, L; Hulín, I

doi:10.20452/pamw.1489

Cited by 13 publications

(7 citation statements)

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“…Increasing evidence indicates that microparticles (MPs) exert multiple prothrombotic actions (31). Compared with controls, monocyte-derived MPs supported faster fibrin formation, 38% higher fibrin network density and higher clot stability (3.8-fold higher turbidity in the presence of tPA) while platelet-derived MPs have no effect on fibrin clot structure (32).…”

Section: Undas: Fibrin Clots and Thrombosismentioning

confidence: 99%

Fibrin clot properties and their modulation in thrombotic disorders

Undas¹

2014

Thromb Haemost

131

136

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Accumulating evidence indicates that accelerated formation of fibrin clots composed of compact, highly-branched networks with thin fibres which are relatively resistant to plasmin-mediated lysis can be commonly observed in patients with venous or arterial thrombosis. This review discusses characteristics of fibrin clot structure and function in patients with various thromboembolic manifestations, in particular myocardial infarction, ischaemic stroke and venous thromboembolism, based on the publications till December 2013. Moreover, factors will be presented that in vivo unfavourably determine altered fibrin clot properties in thrombotic disorders and modalities that can improve clot phenotype.

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Section: Undas: Fibrin Clots and Thrombosismentioning

confidence: 99%

Fibrin clot properties and their modulation in thrombotic disorders

Undas¹

2014

Thromb Haemost

131

136

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“…72 In addition, although EMPs have been suggested to promote cardiovascular disease progression, their protective roles in tissue repair and cell survival have been also reported. 70 Therefore, additional studies are required to understand the detailed mechanisms underlying the complex role of EMPs, including the effect of shear stress on their production and function.…”

Section: Flow-induced Endothelial Microparticlesmentioning

confidence: 99%

Mechanoresponsive Networks Controlling Vascular Inflammation

Bryan

Duckles

Feng

et al. 2014

ATVB

101

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Atherosclerosis is characterized by an accumulation of smooth muscle cells and a buildup of lipid-rich foamy macrophages within the arterial wall, which can lead to lumen narrowing and reduced blood flow or to a complete arterial blockage after plaque rupture.1 Atherosclerosis development is influenced by several factors, including age, sex, cholesterol level, and obesity.2 Despite the systemic nature of these risk factors, atherosclerotic plaque formation is remarkably localized, forming predominantly around arterial branches and the inner curvature of bends. Blood flow is a major influence on localized atherosclerosis development, because the location of plaques correlates with specific flow patterns.3 Regions of the artery wall where blood flow is low or disturbed are prone to atherosclerosis, whereas arterial regions exposed to high flow are protected. 4 Flow affects molecular transport from the bloodstream to the endothelium, because different flow conditions directly influence the local concentration of molecules and their interaction time with the artery wall. In addition to modifying mass transport, blood flow exposes the endothelial layer to wall shear stress (WSS; the friction-like drag between the fluid and the arterial wall surface) and other mechanical actions.5 Flowinduced WSS varies over time in both magnitude and direction in a characteristic pattern that is dependent on the location within the arterial system (Figure 1). 6,7 There are several metrics that can be used to characterize a stress pattern (Figure 1), including time-averaged WSS magnitude, oscillatory shear index (a figure of merit describing the fraction of the profile that the instantaneous WSS opposes the mean WSS direction during one oscillation), angular direction of shear stress, and harmonic frequency components. There is ongoing debate on the relative importance of these parameters in endothelial responses to WSS; however, it is plausible that mechanoreceptors integrate multiple mechanical parameters to convert information on WSS magnitude, frequency, and direction into biochemical signals. 8 Although many interrelated signaling networks have been identified, understanding of mechanically triggered signaling is still developing. Here, we review established, biochemically triggered, inflammatory mechanisms before discussing recent progress in uncovering the role of mechanical cues in inflammatory pathways and possible future directions of research. Please see http://atvb.ahajournals.org/site/misc/ ATVB_in_Focus.xhtml for all articles published in this series. Blood Flow and Vascular InflammationAtherosclerosis is promoted by the recruitment of monocytes from the blood to the vessel wall via interactions with © 2014 American Heart Association, Inc. Abstract-Atherosclerosis is a chronic inflammatory disease of arteries that develops preferentially at branches and bends that are exposed to disturbed blood flow. Vascular function is modified by flow, in part, via the generation of mechanical forces that alter multiple physiological ...

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“…This may be because they constitute only a minor fraction of the MVs in the bloodstream under normal physiological conditions [13]. In pathological processes, the level of leukocyte-derived MVs in blood increases dramatically, so that they are considered to be markers for development of various diseases [7,65]. The phenotype, composition and functions of MVs produced by NK cells are inadequately defined.…”

Section: Introductionmentioning

confidence: 99%

Microvesicles produced by natural killer cells of the NK-92 cell line affect the phenotype and functions of endothelial cells of the EA.Hy926 cell line

et al. 2020

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Резюме. Микровезикулы (МВ)-субклеточные структуры размером от 100 до 1000 нм, продуцируемые клетками в состоянии покоя и активации. МВ могут передавать молекулы клеткам-мишеням, регулировать физиологические процессы, участвовать в патологиях. Микровезикулы лейкоцитарного происхождения, в частности МВ NK-клеток, остаются наименее изученной популяцией МВ. NK-клетки способны изменять функциональную активность эндотелиальных клеток (ЭК), участвуют в регуляции ангиогенеза. Недостаточно изучена способность МВ NK-клеток влиять на функциональное состояние ЭК. Целью настоящего исследования явилось изучение влияния МВ, образуемых естественными киллерами линии NK-92, на фенотип, активность каспаз, пролиферацию и миграцию ЭК линии EA.Hy926. ЭК культивировали в присутствии МВ клеток линии NK-92. При помощи проточной цитофлуориметрии оценивали изменение фенотипа ЭК, передачу внутриклеточного белка из МВ в ЭК, относительную гибель ЭК. При помощи Western blot analysis оценивали экспрессию гранзима B в NK-клетках и их МВ, появление гранзима B в ЭК, экспрессию каспаз, Erk, AKT в ЭК. Также оценивали пролиферацию и миграцию ЭК в присутствии МВ клеток линии NK-92. Установлено значимое различие протеомных профилей клеток линии NK-92 и образуемых ими МВ. Контакт ЭК с МВ клеток линии NK-92 сопровождается развитием следующих событий: 1) экспрессией в ЭК гранзима В; 2) активацией каспазы-9, каспазы-3 и частичной гибелью ЭК; 3) появлением на ЭК панлейкоцитарного маркера CD45; 4) снижением экспрессии CD105 и повышением экспрессии CD34 и CD54; 5) ингибированием миграции ЭК. Передача эндотелиальным клеткам Erk, но не AKT, в составе МВ клеток линии NK-92 в концентрации в 10 раз ниже концентрации, вызывающей гибель ЭК, способствует повышению пролиферации ЭК.

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Microparticles: a component of various diseases

Cited by 13 publications

References 0 publications

Fibrin clot properties and their modulation in thrombotic disorders

Fibrin clot properties and their modulation in thrombotic disorders

Mechanoresponsive Networks Controlling Vascular Inflammation

Microvesicles produced by natural killer cells of the NK-92 cell line affect the phenotype and functions of endothelial cells of the EA.Hy926 cell line

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