Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Abstract

Citation: MORELLI, S., HOLDICH, R. and DRAGOSAVAC, M., 2017. Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification. Journal of Membrane Science, 524, Additional Information:• This paper was accepted for publication in the journal Journal of This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. conditions. The liquid drops were loaded with increasing amount of yeast (3.14 x 10 7 to 3.14 x 10 8 cells/ mL). The stability and uniformity of the emulsions was independent of the cell concentration. PTFE coated hydrophobic membrane produced smaller W/O drops compared to FAS coated membranes. The liquid polymeric droplets were solidified in solid particles using thermal gelation and/or ionic crosslinking, obtaining yeast encapsulated particles sized ~100 µm.The pH sensitive polymer, Eudragit S100, was used as a coating to create gastro resistant particles suitable for intestinal-colonic targeted release. Viability of the released yeast cells was demonstrated using fluorescence probes and checking cell glucose metabolism with time. AbbreviationsW/O emulsion, water in oil

Development and Characterization of Colon-targeting 5-Fluorouracil Multiparticulate Beads

“…However, >75 % of drug was released from the coated beads when pH was gradually raised above 7.0, as ES100 coating membrane get dissolved and leads to a disturbance of the integrity of film [80] . The released profile data corroborated with the findings of Morelli et al as ES100 coating prolonged the drug release upto 24h for the release of yeast cells into the colonic area [81] . Similarly Jena et al has designed ES100 coated Chitosan microspheres which retarded the Capecitabine release upto 24h in colonic region [82] .…”

Membrane Emulsification as an Emerging Method for Lacticaseibacillus rhamnosus GG® Encapsulation

“…The probiotic microcapsules were prepared by membrane emulsification, following the method proposed by Morelli et al ( 2017 ) and Vinner et al ( 2019 ), with modifications. A scheme with the steps of the procedure is shown in Fig.…”

“…Yeast encapsulation using membrane emulsification has also been reported. Morelli et al ( 2017 ) encapsulated Saccharomyces cerevisiae cells using a flat disc metallic membrane with a pore diameter of 30 μm. The size of the droplets (wet suspension) containing S. cerevisiae cells varied between 60 and 340 μm.…”

Water in oil emulsions from hydrophobized metal membranes and characterization of dynamic interfacial tension in membrane emulsification

“…al. [34], two types of hydrophobic nickel membranes were used: PTFE coated (supplied by Micropore Technologies Ltd.) and FAS treated (with the optimised process that is described in this work). Uniform w/o emulsions were produced using both membranes, however further details, disclosed here, show that FAS treatment is an effective and reliable method for producing multiple uniform w/o emulsions.…”

“…Uniform w/o emulsions were produced using both membranes, however further details, disclosed here, show that FAS treatment is an effective and reliable method for producing multiple uniform w/o emulsions. In Figure 8, water based droplets are shown with a formulation reported previously [34], in order to evaluate the performance of a PTFE coated and a FAS treated membrane for multiple emulsions. Operating parameters and cleaning cycles between the runs were equal in order to allow a direct comparison.…”