Microplastics alter nitrous oxide production and pathways through affecting microbiome in estuarine sediments

Chen, Cheng; Pan, Jiongyu; Xiao, Shuxin; Wang, Jiayi; Gong, Xiaoluo; Yin, Guoyu; Hou, Lijun; Liu, Min; Zheng, Yanling

doi:10.1016/j.watres.2022.118733

Cited by 76 publications

(7 citation statements)

References 47 publications

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“…We measured the basal N 2 O production rate in the estuarine sediment (3.31 ± 0.28 nmol N 2 O g −1 h −1 ), which is higher than those in the sediments of Yangtze Estuary (0.04−0.12), 54 highlighting the spatial heterogeneity in N 2 O production in estuarine ecosystems. The dynamics of sedimentary N 2 O are generally determined by a comprehensive network with various contributions from biological (i.e., bacterial and fungal sources) and abiotic (chemo-denitrification) processes.…”

Section: N 2 O Emissionmentioning

confidence: 81%

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Tang

et al. 2022

Environ. Sci. Technol.

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Global estuarine ecosystems are experiencing severe nitrogen pollution and ocean acidification (OA) simultaneously. Sedimentary denitrification is an important way of reactive nitrogen removal but at the same time leads to the emission of large amounts of nitrous oxide (N 2 O), a potent greenhouse gas. It is known that OA in estuarine regions could impact denitrification and N 2 O production; however, the underlying mechanism is still underexplored. Here, sediment incubation and pure culture experiments were conducted to explore the OA impacts on microbial denitrification and the associated N 2 O emissions in estuarine sediments. Under neutral (in situ) conditions, fungal N 2 O emission dominated in the sediment, while the bacterial and fungal sources had a similar role under acidification. This indicated that acidification decreased the sedimentary fungal denitrification and likely inhibited the activity of fungal denitrifiers. To explore molecular mechanisms, a denitrifying fungal strain of Penicillium janthinellum was isolated from the sediments. By using deuterium-labeled single-cell Raman spectroscopy and isobaric tags for relative and absolute quantitation proteomics, we found that acidification inhibited electron transfers in P. janthinellum and downregulated expressions of the proteins related to energy production and conservation. Two collaborative pathways of energy generation in the P. janthinellum were further revealed, that is, aerobic oxidative phosphorylation and TCA cycle and anoxic pyruvate fermentation. This indicated a distinct energy supply strategy from bacterial denitrification. Our study provides insights into fungi-mediated nitrogen cycle in acidifying aquatic ecosystems.

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Section: N 2 O Emissionmentioning

confidence: 81%

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Tang

et al. 2022

Environ. Sci. Technol.

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“…Although the effluent quality of the PN reactor with low-dose PVC-MP exposure (i.e., 0.5 mg/L) remained relatively stable as that of the no-MP control, significant impacts on AORs, N 2 O contributing pathways, and sludge physicochemical properties were detected in this study (Figures –), not to mention the high-dose PVC-MP exposure. Inspired by pioneering research, ,, our work revealed that the three dominant N 2 O production pathways in the PN system were regulated differently by PVC-MP exposure, and ultimately, higher N 2 O emission was observed, which were experimentally determined by a series of cyclic and batch tests, as well as the applications of SP analysis and metabolic inhibitors. The attained results will fill in a knowledge gap relevant to environmental risk assessment of ubiquitous MPs by linking PVC-MP exposure to N 2 O production in the PN system.…”

Section: Implications For Operating Nitritation Systemmentioning

confidence: 99%

“…Chen et al. profoundly analyzed the impacts of three representative MPs on sedimentary N 2 O production and quantitatively examined the regulated contributing pathways through dual-isotope labeling technique and molecular methods . Despite the important progress, knowledge of N 2 O emission from the PN system under MP stress is still limited.…”

Section: Introductionmentioning

confidence: 99%

Polyvinyl Chloride Microplastics Facilitate Nitrous Oxide Production in Partial Nitritation Systems

He,

Liu,

et al. 2024

Environ. Sci. Technol.

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Partial nitritation (PN) is an important partner with anammox in the sidestream line treating high-strength wastewater and primarily contributes to nitrous oxide (N 2 O) emissions in such a hybrid system, which also suffers from ubiquitous microplastics because of the growing usage and disposal levels of plastics. In this study, the influences of polyvinyl chloride microplastics (PVC-MPs) on N 2 O-contributing pathways were experimentally revealed to fill the knowledge gap on N 2 O emission from the PN system under microplastics stress. The long-term results showed that the overall PN performance was hardly affected by the low-dose PVC-MPs (0.5 mg/L) while obviously deteriorated by the high dose (5 mg/L). According to the batch tests, PVC-MPs reduced biomassspecific ammonia oxidation rates (AORs) by 5.78−21.94% and stimulated aerobic N 2 O production by 9.22−88.36%. Further, upon increasing dissolved oxygen concentrations from 0.3 to 0.9 mg O 2 /L, the degree of AOR inhibition increased but that of N 2 O stimulation was lightened. Site preference analysis in combination with metabolic inhibitors demonstrated that the contributions of hydroxylamine oxidation and heterotrophic denitrification to N 2 O production at 0.3 mg O 2 /L were enhanced by 18.84 and 10.34%, respectively, accompanied by a corresponding decreased contribution of nitrifier denitrification. Finally, the underlying mechanisms proposed for negative influences of PVC-MPs were bisphenol A leaching and reactive oxygen species production, which led to more cell death, altered sludge properties, and reshaped microbial communities, further resulting in enhanced N 2 O emission. Overall, this work implied that the ubiquitous microplastics are a hidden danger that cannot be ignored in the PN system.

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“…The use of renewable and biodegradable materials like PLA in electrochemical sensors, combined with the advantageous properties of carbon nanobers, represents a promising area of research for the development of eco-friendly and sustainable sensing platforms. 28,29 Here, a PLA-based membrane doped with CNF has been developed that had a surface area of 3.02 m 2 g −1 , a pore size of 18.77 nm, and a pore volume of 0.0137 cm 3 g −1 . The membrane was further functionalized as a bio-based electrode (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…The use of renewable and biodegradable materials like PLA in electrochemical sensors, combined with the advantageous properties of carbon nanofibers, represents a promising area of research for the development of eco-friendly and sustainable sensing platforms. 28,29…”

Section: Introductionmentioning

confidence: 99%

A polylactic acid–carbon nanofiber-based electro-conductive sensing material and paper-based colorimetric sensor for detection of nitrates

Rai,

Mehrotra,

Gautam

et al. 2024

Anal. Methods

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Microplastics alter nitrous oxide production and pathways through affecting microbiome in estuarine sediments

Cited by 76 publications

References 47 publications

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Polyvinyl Chloride Microplastics Facilitate Nitrous Oxide Production in Partial Nitritation Systems

A polylactic acid–carbon nanofiber-based electro-conductive sensing material and paper-based colorimetric sensor for detection of nitrates

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Microplastics alter nitrous oxide production and pathways through affecting microbiome in estuarine sediments

Cited by 76 publications

References 47 publications

Denitrification and N2O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Denitrification and N2O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Polyvinyl Chloride Microplastics Facilitate Nitrous Oxide Production in Partial Nitritation Systems

A polylactic acid–carbon nanofiber-based electro-conductive sensing material and paper-based colorimetric sensor for detection of nitrates

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Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism

Denitrification and N₂O Emission in Estuarine Sediments in Response to Ocean Acidification: From Process to Mechanism