Microporous membrane and culture medium affect in vitro seedling development of Dalbergia nigra (Vell.) Ex Benth. (Fabaceae) by modulation of the protein profile and accumulation of ethylene and CO2

Carrari-Santos, Renan; Vettorazzi, Rosana Gobbi; Pinto, Vitor Batista; Sena, Edinaldo de Oliveira Alves; Oliveira, Jurandi Gonçalves de; Campostrini, Eliemar; Silveira, Vanildo; Santa–Catarina, Claudete

doi:10.1007/s11240-023-02492-9

Cited by 5 publications

(1 citation statement)

References 86 publications

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“…In the photomixotrophic system with a lower gas exchange rate (or without natural ventilation), the microenvironment of the flask is the factor with the greatest influence on the growth and development, since carbon is made available through the supply of sucrose in the medium [33][34][35]. Undeniably, sealing the bottles (rigid lids or without membranes) provides high humidity inside them, greater accumulation of the phytohormone ethylene, reduced gas exchange, and low CO 2 concentration [36][37][38][39]. These factors lead to the development of plants with morphophysiological disorders, such as leaf formation displaying mesophyll with large intercellular spaces, poorly developed vascular system, lack of epicuticular wax formation, and non-functional stomata [16,18,19,40].…”

Section: Discussionmentioning

confidence: 99%

Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

Silva,

Soares,

Silva

et al. 2024

Horticulturae

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Eryngium foetidum L., a biennial herb with diverse applications in food and traditional medicine, holds economic and pharmacological significance. Given its growing commercial interest, implementing biotechnological approaches like plant tissue culture is vital for sustainable propagation and metabolite production. In this study, we investigated the in vitro photoautotrophic potential of Eryngium foetidum, examining growth, chlorophyll a fluorescence, photosynthetic pigments, and anatomical features under sucrose concentrations (0 and 30 g L−1) and gas exchange rate (14 and 25 μL L−1 s−1 CO2). Acclimatization and survival rates of plants after ex vitro transfer were also assessed. Eryngium foetidum exhibited robust growth in both photoautotrophic and photomixotrophic conditions, with natural ventilation significantly enhancing plant development. Chlorophyll a fluorescence and photosynthetic performance were influenced by sucrose and gas exchange, highlighting the importance of these factors in plant micropropagation. Moreover, the species demonstrated remarkable plasticity during acclimatization, with high survival rates and rapid inflorescence development. The research provides valuable insights into optimizing in vitro cultivation conditions for Eryngium foetidum, emphasizing the potential for large-scale clonal propagation and exploring secondary metabolites. The observed phenotypic plasticity underscores the adaptability of the species to diverse environments. These biotechnological strategies open avenues for future studies, including the application of elicitors for enhanced secondary metabolite production.

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