Micropropagation and Ex Vitro Rooting of Wolfberry

Abstract

Additional index words. Lycium barbarum, axillary shoot proliferation, in vitro and ex vitro rooting, acclimatization, growth regulatorsAbstract. An accurate protocol for the in vitro propagation of a commercial wolfberry (Lycium barbarum L.) cv. Nixia 1 has been developed through axillary shoot proliferation. Driver and Kuniyuki Walnut (DKW) medium supplemented with 6-benzylaminopurine (BAP; 0.5 mg/L) and sucrose 3% w/v gave the best results compared with other basal media tested, with significantly improved production of multiple shoots direct from nodal segment explants, resulting in an average of 6.73 shoots/explant with an average of 7.45 nodes/shoot that would potentially form new explants. Rooting of shoot explants was carried out both in vitro and ex vitro with 0.5 and 1 mg/L of indole-3-butyric acid (IBA), with or without adding putrescine (160 mg/L). In all cases, rooting efficiency resulted very high, and putrescine was effective only when combined with a low concentration of auxin. Plantlets were hardened off in jiffy pots under greenhouse conditions, with a survival rate of more than 90%. Ex vitro rooting, performed by dipping in an aqueous solution of IBA 100 mg/L, is the preferred technique not only because rooting and acclimatization are very high but also reducing micropropagation to one phase is more economical.

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

“…Moreover, the best shoot proliferation of L. depressum was achieved at a low concentration of BA (6-benzyl adenine) and rooted in full-strength MS medium (Murashige and Skoog medium) supplemented with IBA (indole-3-butyric acid) with a high survival rate [10]. Micropropagation protocols were also developed in L. barbarum [11] and L. chinense [12].…”

Micropropagation, rooting, and acclimatization of two cultivars of goji (Lycium chinense)

“…Because of its high nutraceutical and pharmaceutical value (Dănăilă-Guidea et al, 2015;Kruczek and Ochmian, 2016;Kruczek et al, 2020a), the production of goji has been rapidly increasing. Wolfberry plants are traditionally propagated trough their seeds and usually encounter problems related to sexual propagation, especially low germination, lack of clonal expansion, and irregular agronomical performance (Silvestri et al, 2018). Clonal micropropagation by in vitro culture is the best alternative to overcome these barriers and has great potential for rapid multiplication and production of high-quality plant material.…”

“…A cluster analysis conducted using Ward's method (Figure 1b) showed three separate groups (a-c) with a similar influence of medium composition on the in vitro rooting of goji 'No 1' and 'New Big'. Silvestri et al (2018) showed that the percentage of rooted shoots of L. barbarum 'Nixia 1' was higher (87-95%) in ½ MS with 1% sucrose and indole butyric acid (IBA) with or without putrescine and the acclimatization rate of the plants in soil range from 88.7% to 95.1%. Tabăra (2017) rooted L. barbarum (boxthorn) with a higher success rate (90-95%) of explants transferred to a solid substrate of peat and sand.…”

Propagation of Dracaena Umbraculifera Plant by Tissue Culture Technique

“…Dewir et al (2018) on Conocarpus erectus mentioned that the best rooting medium was MS supplemented with 1 mg/l IBA. Silvestri et al (2018) on Lycium barbarum found that rooting of shoots was carried out in vitro with 0.5 -1 mg/l of IBA. Dewir et al (2019) on Dracaena ombet found that 100% rooting was obtained using half strength MS medium supplemented with 0.5 or 1 mg/l IBA.…”