Micropropagation and in vitro flowering of endemic and endangered plant Ceropegia attenuata Hook

Abstract

Factors affecting in vitro propagation were evaluated for Ceropegia attenuata Hook., an endemic and endangered plant having ornamental potential but a limited reproductive capacity. Rapid shoot multiplication from nodal explants was established using varying concentrations of cytokinins and auxins either alone or in combinations. The highest frequency of shoot induction was achieved when nodal explants were inoculated on Murashige and Skoog (MS) medium supplemented with 13.31 μM 6-benzylaminopurine with a mean of 12.9±0.5 shoots per explant. High concentrations of TDZ (6.81-11.35 μM) and KN (6.78-11.61 μM) resulted in stunted and vitrified shoots. Factors implicated in the promotion of floral transition of the C. attenuata have been identified which are 4-amino-3, 5, 6-trichloropicolinic acid (picloram), 6-benzylaminopurine, sucrose and photoperiod. The highest frequency of flowering (100%) was obtained when axillary shoot explants were transferred to MS medium supplemented with picloram (4.14 μM) within 4 weeks of culture. Transfer of in vitro regenerated shoots to half strength MS medium with 2.46 μM indole-3-butyric acid (IBA) showed maximum root induction. The in vitro grown plantlets were successfully acclimatized in the glasshouse with 85% of survival and showed normal development. The developed protocol provided a simple, cost-effective approach for the conservation of endangered plant C. attenuata for replenishing its declining populations.

Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

“…Similarly, the higher concentrations of IBA, IAA and NAA could induce the callus formation rather than rooting. Thus, the present study is in agreement with the works of Baksha et al (2007), and Chavan et al (2011).…”

“…When in vitro derived shoots of C. at tenuata were cultured on half strength MS medium having 2.46 µM IBA, they could induce the highest percentage (100) of root induction and number of (9.8) roots (Chavan et al 2011). The results of the present study are in agree ment with the works of Chavan et al (2013) and Chavan et al (2011).…”

“…The highest percentage of rooting (96±1.9 %) was ob served, when microshoots of C. panchganiensis were cultured on half strength MS medium supplemented with 7.36 µM IBA, where 9.3 roots/microshoots and 3.6 cm root length Chavan et al (2013). When in vitro derived shoots of C. at tenuata were cultured on half strength MS medium having 2.46 µM IBA, they could induce the highest percentage (100) of root induction and number of (9.8) roots (Chavan et al 2011). The results of the present study are in agree ment with the works of Chavan et al (2013) and Chavan et al (2011).…”

“…Similarly, reported that the higher concentrations of IBA, NAA and IAA resulted in swelling and callus formation at the base of the shoots of C. odorata and C. maccannii. Chavan et al (2011) stated that MS medium was sup plemented with increasing concentration of IAA could produce the callus formation in C. attenuata during in vitro root formation. Baksha et al (2007) explained that the in creasing concentrations of (1.0 mg/l) NAA and IBA could decrease root induction percentage and number of roots per explant, however, it could increase callus formation too.…”

In Vitro Propagation of Ceropegia anjanerica Malpure et al.: A Rare Endemic Plant from Maharashtra

“…The habitat degradation, over exploitation of root tubers and anthropogenic activities are major threats to the survival of this species. Besides, the scarcity of pollinators and poor seed setting are other major constraints in its natural propagation, leading to a continuous depletion of its natural population [10]. The in situ conservation effort has had the limited impact on halting the decline in the population [11], so there is a necessity to set successful micropropagation protocol for large-scale multiplication and conservation of this plant species.…”