Micropropagation and in vitro flowering in Pentanema indicum Ling

Sivanesan, Iyyakkannu; Jeong, Byoung Ryong

doi:10.5511/plantbiotechnology.24.527

Cited by 17 publications

(9 citation statements)

References 22 publications

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“…Higher frequency of flowering was observed when the medium was supplemented with 3% (w/v) sucrose than with 1, 2, 4 and 5% (Table 5). This result coincides with earlier reports in Pentanema indicum (Sivanesan and Jeong 2007), Pisum sativum (Franklin et al 2000) and Gentiana trifolia (Zhang and Leung 2002). Sucrose and cytokinins interact with each other for floral induction in Sinapis alba by moving between shoot and root (Havelange et al 2000).…”

Section: Resultssupporting

confidence: 95%

Plant growth regulators, adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering of Anethum graveolens

Jana

Shekhawat

2010

Acta Physiol Plant

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In vitro regeneration protocol for Anethum graveolens (Apiaceae) was developed using leaf explants. MS basal medium used in experiments was augmented with various hormones for caulogenic and rhizogenic response. The optimum callus induction (100%) was obtained by leaf explants on MS media fortified with BA (0.5 mg l -1 ) singly and in combination with NAA (0.1 and 0.2 mg l -1 ). BA at 0.5 mg l -1 , KN at 1.0 mg l -1 and NAA at 0.1 mg l -1 induced highest number of multiple shoots (10.0 ± 0.25) per explant and they also showed in vitro flowering within 3 weeks of culture. Influence of adenine sulfate on regeneration frequency of callus was evaluated. The highest frequency of rooting (100%) with 6.0 ± 0.25 roots per explants was obtained in one-fourth strength MS medium supplemented with 1/4 MS ? IBA 0.5 mg l -1 within 4 weeks of transfer to the rooting medium. In vitro flowering (35%) was obtained with MS fortified with BA alone and also in combination with KN and NAA (5.3 ± 0.42 flowers per explants). In vitro flowering response was tested with different carbohydrates (fructose, glucose, mannose and sorbitol) and optimized. Hardening was successfully attained under controlled conditions inside the plant tissue culture room. The proposed method could effectively be applied for the conservation and clonal propagation to meet the pharmaceutical demands of this medicinally important species.

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Section: Resultssupporting

confidence: 95%

Plant growth regulators, adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering of Anethum graveolens

Jana

Shekhawat

2010

Acta Physiol Plant

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“…Higher concentrations of BA (> 8.8 µM) inhibited callus induction. Similar reports have been observed on Pentanema indicum (Sivanesan and Jeong 2007). Callus formation on cytokinin enriched medium was found more frequent in species with strong apical dominance (Preece et al 1991).…”

Section: Resultssupporting

confidence: 84%

In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

Jana¹,

Shekhawat²

2012

Biologia plant.

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An efficient in vitro regeneration protocol was developed for medicinally important aromatic plant Anethum graveolens. Nodal segments were cultured onto Murashige and Skoog (MS) basal medium supplemented with different auxins and cytokinins singly as well as in combinations. The optimum callus induction (93.33 %) was obtained on medium fortified with 2.2 µM N 6 -benzyladenine (BA) and 0.21 µM α-naphthaleneacetic acid. The best shoot regeneration (85.7 %) with 12.86 shoots per explant was achieved in two weeks when callus was subcultured on MS medium amended with 2.2 µM BA and 1.85 µM kinetin. The average length of regenerated shoots varied from 3.15 to 4.8 cm. The rooting of regenerated shoots was nearly 100 % on ¼ MS augmented with 4.9 µM indolebutyric acid with a maximum root length of 5.1 cm. Plantlets were successfully acclimatized with 60 % survival rate. During organogenesis, catalase and ascorbate peroxidase activity increased while superoxid dismutase activity decreased. Clonal fidelity of in vitro raised plants has been checked by random amplified polymorphic DNA using 10 selected decamer primers. It has been found that regenerated plants are true to type plants.

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“…The formation of seeds requires flowering phase in such commercial oil crops. Therefore, in vitro propagation along with in vitro flowering will not only help in production of better quality of oil plants but will also ensure shortening of the breeding cycle (Sivanesan and Jeong 2007;Kielkowska and Havey 2012;Haque and Ghosh 2013a) and studying the developmental pattern of flowers from vegetative to floral phase (Huang et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

Baghel

Bansal

2014

Physiol Mol Biol Plants

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Apical and axillary buds of Guizotia abyssinica Cass., isolated from seedlings raised in vitro, were cultured. High frequency of shoot regeneration was achieved on MS medium with BAP (1 mgl(-1)). Effect of BAP, Kn and GA3 applied successively in culture on shoot regeneration and flower bud formation has been studied. The shoots differentiated in cultures elongated on this medium. These rooted subsequently on half strength MS medium. The shoots flowered in vitro on MS medium with a combination of BAP (0.1mgl(-1)) + GA3 (0.1 mgl(-1)). The plantlets thus formed were successfully hardened with 90 % survival.

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Micropropagation and in vitro flowering in Pentanema indicum Ling

Cited by 17 publications

References 22 publications

Plant growth regulators, adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering of Anethum graveolens

Plant growth regulators, adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering of Anethum graveolens

In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

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