Micropropagation and in vitro polyploidisation of<i>Siphonochilus aethiopicus</i>(wild ginger) and its effect on selected horticultural characteristics

Hannweg, K.; Visser, G.; Sippel, A.; Bertling, I.

doi:10.17660/actahortic.2016.1113.26

Cited by 2 publications

(1 citation statement)

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“…In the present study, tetraploid plantlets had shorter leaf, stem, and root lengths and thicker stem and root diameters as well as increased fresh weights compared with the diploid, representing a phenomenon called the Gigas effect (Knight and Beaulieu, 2008); in other words, chromosome doubling generally causes increased organ size (Osborn et al, 2003). Therefore, tetraploid plants had significantly higher biomass production (Corrêa et al, 2016;Hannweg et al, 2016). Additionally, stomatal size was significantly increased but stomatal density was decreased in tetraploid plants.…”

Section: Discussionmentioning

confidence: 55%

Changes in Morphological Characteristics, Regeneration Ability, and Polysaccharide Content in Tetraploid Dendrobium officinale

Pham¹,

Li²,

Guo³

et al. 2019

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Dendrobium officinale Kimura et Migo is a famous traditional Chinese medicinal plant. It produces various phytochemicals, particularly polysaccharides, which have nutraceutical and pharmaceutical values. To increase its biomass production and polysaccharide content, our breeding program has generated a series of polyploid cultivars through colchicine treatment of protocorm-like bodies (PLBs). The present study compared two tetraploid cultivars, 201-1-T1 and 201-1-T2, with their diploid parental cultivar, 201-1, in an established in vitro culture system. Tetraploid ‘201-1-T1’ and ‘201-1-T2’ had shorter leaves and shorter and thicker stems and roots, and they produced higher biomass compared with the diploid cultivar. The length and width of stomata significantly increased, but stomatal density decreased in tetraploid cultivars. The PLB induction rates from the stem node explants of the tetraploid cultivars were significantly higher than those of diploid. However, the PLB proliferation of tetraploids was lower than that of the diploid. The mean number of plantlets regenerated from tetraploid PLBs was also lower than that of the diploid after 4 months of culture. Polysaccharide contents in stems, leaves, and roots of 6-month-old tetraploid plantlets were significantly higher than those of diploids. The polysaccharide content in the stem of ‘201-1-T1’ was 12.70%, which was a 2-fold increase compared with the diploid cultivar. Our results showed that chromosome doubling could be a viable way of improving D. officinale in biomass and polysaccharide production.

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Section: Discussionmentioning

confidence: 55%