Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

Abstract

This study's aim was to establish a protocol for the micropropagation of G. skinneri using temporary immersion system (TIS). Different concentrations of 6-Benzylaminopurine (0, 1, 2, and 3 mg L −1 ), three different systems of cultivate semi-solid (SS) and liquid media under partial (PI) and temporary immersion systems (TIS), different compositions of the inorganic salts, and the number of subcultures were evaluated. The results showed a maximum of 16.56 shoots per explant obtained through TIS, adjusting all the parameters evaluated in our study. One higher number of shoots per explant was observed in the micropropagation of G. skinneri TIS compared to SS and PI. While the use of 3 mg L −1 of BAP + MS (Murashige and Skoog) media was better than 3 mg L −1 of BAP VW (Vacint and Went) for the generation of a greater number of shoots per explant, 6.33 and 2.72, respectively. The immersion frequency of 2 min every 4 h allowed the production to be scaled to 8.54 shoots per explant. While it was necessary to perform three subcultures every 30 days, to obtain 16.56 shoots per explant, a rooting phase was not required due to the generation of adventitious roots during the different subcultures. However, a phase of elongation of the regenerated plants with ½ MS + GA3 (gibberellic acid) was needed to guarantee 100% survival in the process of acclimatization. In conclusion, this plant production system can be applied for the commercial micropropagation of this species for ornamental purposes, as well as for its reintroduction in protected natural areas.

Effects of culture methods and plant growth regulators on in vitro propagation of Brassavola nodosa (L.) Lindl. hybrid

“…In addition, no published reports are available on in vitro establishment and propagation of B. nodosa. Most recently, some studies have showed improved efficiency of orchid micropropagation in liquid culture (Young et al 2000;Leyva-Ovalle et al 2020). Liquid culture systems may offer some advantages over semi-solid media in in vitro systems improving plant growth and development and providing higher rates of multiplication and the potential of reduction in manipulation and labor costs via automation (Ziv 2000).…”

“…(Wu et al 2007), Guarianthe(Leyva-Ovalle et al 2020), and Cattleya (Ekmekçigil et al 2019). These results warrant further investigation on the potential of TIS bioreactors for the micropropagation of B. nodosa.…”

A Temporary Immersion System to Improve Cannabis sativa Micropropagation

“…Recently, several studies have shown that TIS have numerous advantages as regards the semi-solid methods (Vidal and Sánchez, 2019 ). These systems have been successfully used for micropropagation of Stevia rebaudiana (Melviana et al, 2021 ), Colocasia esculenta L. Schott (Mancilla-Álvarez et al, 2021 ), Agave angustifolia (Monja-Mio et al, 2021 ), Rosmarinus officinalis L. (Villegas-Sánchez et al, 2021 ), Dracocephalum forrestii (Weremczuk-Jezyna et al, 2020 ), Guarianthe skinneri (Leyva-Ovalle et al, 2020 ), and the number is increasing steadily. In our laboratory, we have developed protocols using bioreactor propagation systems with liquid medium for axillary shoots of chestnut, alder and willow (Vidal et al, 2015 ; Cuenca et al, 2017 ; Regueira et al, 2018 ; San José et al, 2020 ; Gago et al, 2021 ), and somatic embryos of Quercus robur (Mallón et al, 2012 , 2013 ).…”

Micro‐morpho‐anatomical mechanisms involve in epiphytic adaptation of micropropagated plants of Vanda tessellata (Roxb.) Hook. ex G. Don

“…Micropropagation offers an alternate solution for the production of a large number of plants within a short time (Leyva‐Ovalle, Bello‐Bello, Murguía‐González, Núñez‐Pastrana, & Ramírez‐Mosqueda, 2020). The ambient in vitro culture environment allows plants to grow in aseptic conditions with reduced light intensity, higher humidity, rich carbohydrate, nutrients, and growth regulators (Hazarika, 2003; Teixeira da Silva et al, 2015).…”