Micropropagation of Pistachio

In vitro micropropagation of almond (Amygdalus communis L. cv. Nonpareil)

“…To obtain stocks without viruses, it is necessary to use seeds without viruses as rootstocks. For this purpose, many in vitro experiments have been conducted for these economically important fruit trees (Kester and Asay, 1975;Wang and Hu, 1980;Hisajima, 1982;Barghchi and Alderson, 1983;Maynard et al, 1991;Gürel and Gül en, 1998;Onay et al, 2003;Adıyaman et al, 2004;Tilkat et al, 2005). This study used homozigot diploid Nonpariel, which is an economical important woody tree, and the effects of different PGRs on in vitro micropropagation were investigated.…”

Novel Methods in Micropropagation of Pistachio

“…The in vitro initiation of roots for the regenerated pistachio shoots was found to be especially dependent upon the auxin type and the concentration (Barghchi and Alderson 1989 ;Par fi tt and Almehdi 1994 ;Onay 2000b ;Ozden Tokatli et al 2005 ;Tilkat 2006 ) . Successful reports on the tissue culture of adult P. vera L. produced somatic plantlets, but obstacles pertaining to the low incidence of the rooting of plantlets reproducibility during the rooting regime and the poor establishment of in vitro propagated plants after acclimatization as a result of fungal infections have been reported (Onay 2000b ;Tilkat et al 2008 ) .…”

“…Successful reports on the tissue culture of adult P. vera L. produced somatic plantlets, but obstacles pertaining to the low incidence of the rooting of plantlets reproducibility during the rooting regime and the poor establishment of in vitro propagated plants after acclimatization as a result of fungal infections have been reported (Onay 2000b ;Tilkat et al 2008 ) . Abnormality in vitro morphologies related to the callus production of cultured plant tissues is also a hindrance to commercial in vitro plant propagation as losses of up to 50% can be observed (Tilkat 2006 ) .…”

Propagation of pistachio applying in vitro culture techniques

“…Lack of rooting and synchrony, or a scarce root number per shoot were frequently observed during pistachio propagation (Onay, 2000;Tilkat et al, 2009b;García et al, 2010). For pistachio AD15, although good rooting rate was achieved (90%), acclimatization response was poor.…”

“…In vitro culture of Pistacia juvenile explants (Barghchi and Alderson, 1989;Ozden-Tokatli et al, 2005;Benmahioul et al, 2009;Yildirim, 2012) or adult explants (Onay, 2000;Tilkat et al, 2009a) from local Turkish cultivars 'Siirt' or 'Antep' have been previously achieved. However, attempts to reproduce these protocols with the cultivars studied here failed.…”