Micropropagation, phytochemistry and biological activity of the critically endangered Mammillaria herrerae Werdermann

Song, Kihwan; Kang, Hyun-Koo; Ak, Güneş; Zengin, Gökhan; Cziáky, Zoltán; Jekő, József; Kim, Doo Hwan; Lee, O New; Sivanesan, Iyyakkannu

doi:10.1016/j.sajb.2020.11.025

Cited by 5 publications

(2 citation statements)

References 49 publications

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“…Additionally, BAP is the most effective cytokinin for shoot multiplication from the explant of Asiatic Lilium [25]. More recent studies reported that different concentrations of BAP produced significant responses for the shoot length, and/or the number of leaves of Trigonella foenum-graecum L. at 2.0 mg/L [26] and Mammillaria herrerae Werdermann at 0.1 mg/L [27] as well as of Salvia officinalis L. at 0.5 mg/L combined with 0.1 mg/L of the auxin IAA [28].…”

Section: Shooting Behaviourmentioning

confidence: 99%

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Sayed

Marzouk²,

Sokkar

2022

Egypt. J. Chem.

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The present study considered the in vitro propagation of Brunfelsia pauciflora utilizing various cytokinins, as well the estimation of the antioxidant, total phenolics (TPs), and flavonoids (TFs) for shootlets of each treatment. The explants were sterilized with 0.2% mercuric chloride (20 min) and then cultured on Murashige and Skoog medium (MS) supplemented with three cytokinins; benzyl aminopurine (BAP), 6-Furfuryl-aminopurine (Kin), and isopentenyl adenine (2iP) at different concentrations. The highest shootlets number and fresh weight were obtained using 1.0 mg/L BAP, while shootlets length is increased using 2.0 mg/L kin. Additionally, significant numbers of leaves were observed for the plantlets supplemented with kin (1.0 & 3.0 mg/L) and 2iP (2.0 & 3.0 mg/L). The shootlets were rooted one month later with different strengths (full, ¾, ½, or ¼) on MS media without or with 1.0g/L activated charcoal (AC). Shootlet cultured on full-strength MS medium with AC gave the longest root, while the greatest numbers of roots were recorded using ¾ strength without AC. All the rooted shootlets were acclimatized on peat moss and recorded with the longest root length and greatest leaves and roots numbers. Alternatively, the methanolic extract of shootlets supplemented with 2iP (3.0 mg/L) yielded the maximum antioxidant effect (89.89 ± 0.28 mg Trolox /g extract), TPs (57.574±0.82 mg Gallic/g extract), and TFs (46.146 ± 0.31 mg Catechin/g extract) compared with the control (MS free) and mother plant. In conclusion, the antioxidant potential was shown to be strongly correlated with the TFs and the number of leaves /shootlet.

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Section: Shooting Behaviourmentioning

confidence: 99%

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Sayed

Marzouk²,

Sokkar

2022

Egypt. J. Chem.

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“…In vitro technology includes different methodologies such as plant cell, tissue, and organ culture. Under in vitro conditions, plants are propagated in sterile and homogeneous conditions that contribute to the conservation of endangered species [27][28][29]. For in vitro derived plants and cells, it has been demonstrated that the obtained biomass has the biosynthetic capacity for producing most of the bioactive metabolites present in mother plants [30], and that the accumulation of compounds can be enhanced by using elicitor or precursor feeding treatments [3,31].…”

Section: Introductionmentioning

confidence: 99%

Alkaloid and Nitrogenated Compounds from Different Sections of Coryphantha macromeris Plants and Callus Cultures

Viera-Escareño,

Perez-Molphe Balch,

Gómez-Aguirre

et al. 2023

Applied Sciences

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One of the distinctive characteristics of cacti species is the presence of alkaloids. Alkaloids are nitrogenated molecules with hallucinogenic and pharmacological properties in humans and other animals. Plant cell, tissue, and organ culture have emerged as an effective tool for investigating the biosynthesis of a variety of functional metabolites and for studying the preservation of endangered plant species. In this study, we examined the alkaloid and nitrogenated compound profiles of the aerial and radicular sections of Coryphantha macromeris plants that were cultivated in both greenhouse and in vitro conditions. Additionally, we analyzed the callus cultures generated from stem discs. To perform these analyses, Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS) was utilized. Under the working parameters, 78 compounds were detected, and 61 of them were identified. Among the identified compounds, the in vitro plants presented 24 compounds, greenhouse plants a total of 21 compounds, and callus tissue 16 compounds. On the other hand, 7 compounds (laurydiethanolamine, toluic acids, and their derivatives) were detected in all systems, suggesting that these metabolites may serve as markers to help find the authenticity of C. macromeris preparations, and that, plant and cell-tissue cultures with this plant species are suitable for the biosynthesis of the selected compounds. In addition, our research suggests that no alkaloids with reported psychotropic properties are present in C. macromeris.

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Mass micropropagation and in vitro flowering of Mammillaria vetula ssp. gracilis var. Arizonica Snowcap

Lopez-Granero,

Arana,

Regalado

et al. 2023

Plant Cell Tiss Organ Cult

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Micropropagation, phytochemistry and biological activity of the critically endangered Mammillaria herrerae Werdermann

Cited by 5 publications

References 49 publications

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Production of phenolics and flavonoids with antioxidant evaluations of Brunfelsia pauciflora (Cham. &Schltdl.) Benth. through in vitro propagated cultures.

Alkaloid and Nitrogenated Compounds from Different Sections of Coryphantha macromeris Plants and Callus Cultures

Mass micropropagation and in vitro flowering of Mammillaria vetula ssp. gracilis var. Arizonica Snowcap

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