MicroRNA-124-3p affects proliferation, migration and apoptosis of bladder cancer cells through targeting AURKA

Yuan, Qiuyue; Sun, Tingge; Ye, Feng; Kong, Weisheng; Jin, Hai

doi:10.3233/cbm-160427

Cited by 34 publications

(31 citation statements)

References 33 publications

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“…MiR-124, which was identified as a tumor suppressor, has been confirmed to be downregulated in cancers and investigated for its role in regulating cell proliferation and apoptosis by targeting different genes in distinct types of cancer [28–30]. Moreover, miR-135b was identified as a biomarker for colorectal cancer [31] and reported to modulate apoptosis by targeting large tumor-suppressor kinase 2 [32].…”

Section: Discussionmentioning

confidence: 99%

Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy

Gong

Xie

Liu

et al. 2017

Journal of Diabetes Research

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The pathological mechanisms of diabetic retinopathy (DR), a leading cause of blindness in adults with diabetes mellitus, remain incompletely understood. Because microRNAs (miRNAs) represent effective DR therapeutic targets, we identified aberrantly expressed miRNAs associated with cellular dysfunction in early DR and detected their potential targets. We exposed human retinal endothelial cells (HRECs) and a cell line of retinal pigment epithelial (RPE) cells to high glucose (25 mmol/L, 1–7 days) to mimic DR progression and used streptozotocin-injected rats (4–8 weeks) for an in vivo diabetes model. HREC/RPE viability decreased after 24 h incubation and diminished further over 6 days, and Hoechst staining revealed hyperglycemia-induced HREC/RPE apoptosis. Although miR-124/-125b expression decreased with DR progression in vitro and in vivo, miR-135b/-199a levels decreased in retinal cells under hyperglycemia exposure, but increased in diabetic retinas. Moreover, miR-145/-146a expression decreased gradually in high-glucose-treated HRECs, but increased in hyperglycemia-exposed RPE cells and in diabetic rats. Our findings suggested that aberrant miRNA expression could be involved in hyperglycemia-induced retinal-cell dysfunction, and the identified miRNAs might vary in different retinal layers, with expression changes associated with DR development. Therefore, miRNA modulation and the targeting of miRNA effects on transcription factors could represent novel and effective DR-treatment strategies.

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Section: Discussionmentioning

confidence: 99%

Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy

Gong

Xie

Liu

et al. 2017

Journal of Diabetes Research

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“…Guo et al reported that AURKA overexpression in BCa was strongly associated with tumor stage and grade; additionally, AURKA upregulation was correlated with poor overall survival [81]. In xenograft BCa models, the therapeutic potential of AURKA blockade was demonstrated [82].…”

Section: The Hub Genes Potentially Associated With the Clinical Prognmentioning

confidence: 99%

Identification of Key Biomarkers in Bladder Cancer: Evidence from a Bioinformatics Analysis

2020

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Bladder cancer (BCa) is one of the most common malignancies and has a relatively poor outcome worldwide. However, the molecular mechanisms and processes of BCa development and progression remain poorly understood. Therefore, the present study aimed to identify candidate genes in the carcinogenesis and progression of BCa. Five GEO datasets and TCGA-BLCA datasets were analyzed by statistical software R, FUNRICH, Cytoscape, and online instruments to identify differentially expressed genes (DEGs), to construct protein‒protein interaction networks (PPIs) and perform functional enrichment analysis and survival analyses. In total, we found 418 DEGs. We found 14 hub genes, and gene ontology (GO) analysis revealed DEG enrichment in networks and pathways related to cell cycle and proliferation, but also in cell movement, receptor signaling, and viral carcinogenesis. Compared with noncancerous tissues, TPM1, CRYAB, and CASQ2 were significantly downregulated in BCa, and the other hub genes were significant upregulated. Furthermore, MAD2L1 and CASQ2 potentially play a pivotal role in lymph nodal metastasis. CRYAB and CASQ2 were both significantly correlated with overall survival (OS) and disease-free survival (DFS). The present study highlights an up to now unrecognized possible role of CASQ2 in cancer (BCa). Furthermore, CRYAB has never been described in BCa, but our study suggests that it may also be a candidate biomarker in BCa.

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“…The tumor suppressor miR-124 is downregulated in cancers and plays a role in regulating cell proliferation and apoptosis by targeting different genes in distinct types of cancer. 33,34 Gong et al also reported that the downregulation of miR-125b correlates with the upregulation of SP1, a key transcription factor involved in various types of diseases that plays vital roles in angiogenesis, inflammation, cell proliferation, migration/ invasion, and survival. 35,36 In summary, based on these previous studies and our results, we hypothesized that MALAT1 might play important roles in DR by regulating Sag and Guca1a through miR-124-3p and regulating Pde6g through miR-125b-5p.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of Weighted Genes in Diabetic Retinopathy

You

Zhang

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Intricate signaling networks and transcriptional regulators translate pathogen recognition into defense responses. The aim of this study was to identify the weighted genes involved in diabetic retinopathy (DR) in different rodent models of diabetes. METHODS. We performed a gene coexpression analysis of publicly available microarray data, namely, the GSE19122 dataset from the Gene Expression Omnibus database. We conducted gene coexpression analysis on the microarray data to identify modules of functionally related coexpressed genes that are differentially expressed in different rodent models. We leveraged a richly curated expression dataset and used weighted gene coexpression network analysis to construct an undirected network. We screened 30 genes in the most closely related module. A protein-protein interaction network was constructed for the genes in the most related module using the Search Tool for the Retrieval of Interacting Genes. Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed for the 30 genes. RESULTS. Five visual perception-related genes (Pde6g, Guca1a, Rho, Sag, and Prph2) were significantly upregulated. Based on the competing endogenous RNA hypothesis, a link between the long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and visual perception-related mRNAs was constructed using bioinformatics tools. Six potential microRNAs (miR-155-5p, miR-1a-3p, miR-122-5p, miR-223-3p, miR-125b-5p, and miR-124-3p) were also screened. CONCLUSIONS. MALAT1 might play important roles in DR by regulating Sag and Guca1a through miR-124-3p and regulating Pde6g through miR-125b-5p.

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MicroRNA-124-3p affects proliferation, migration and apoptosis of bladder cancer cells through targeting AURKA

Abstract: MiR-124-3p has significant impact on proliferation, migration and apoptosis of BC cells by targeting AURKA.

Cited by 34 publications

References 33 publications

Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy

Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy

Identification of Key Biomarkers in Bladder Cancer: Evidence from a Bioinformatics Analysis

Bioinformatics Analysis of Weighted Genes in Diabetic Retinopathy

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