MicroRNA-145 promotes the epithelial-mesenchymal transition in peritoneal dialysis-associated fibrosis by suppressing fibroblast growth factor 10

Wu, Jiayu; Huang, Q.; Li, Peilin; Wang, Yuxian; Zheng, Chenghao; Lei, Xianghong; Li, Shuting; Gong, Wangqiu; Yin, Bohui; Luo, Congwei; Xiao, Jing; Zhou, Weidong; Zhou, Xu; Chen, Yihua; Peng, Fenfen; Long, Haibo

doi:10.1074/jbc.ra119.007404

Cited by 21 publications

(13 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, miR-145 was found to reduce HCC invasion by inhibiting EMT. Our findings are inconsistent with previous studies where miR-145 was found to promote EMT by regulating Fibroblast Growth Factor 10 (FGF10) [ 34 ]. However, our findings were consistent with the results published by Chen et al where miR-145 was found to inhibit the invasion and metastasis of HCC cells [ 35 ].…”

Section: Discussioncontrasting

confidence: 99%

Circular RNA hsa_circ_0003288 induces EMT and invasion by regulating hsa_circ_0003288/miR-145/PD-L1 axis in hepatocellular carcinoma

Zhang

Liang

et al. 2021

Cancer Cell Int

View full text Add to dashboard Cite

Background Epithelial-mesenchymal transition (EMT) has been associated with wound healing, tumorigenesis, and metastasis. Circular RNAs (circRNAs) are functional non-coding RNAs involved in multiple human cancers. However, whether and how circRNAs contribute to the EMT in hepatocellular carcinomas (HCC) remains to be deciphered. In this study, we investigated the regulation and function of hsa_circ_0003288 on programmed death-1 ligand 1 (PD-L1) during EMT and HCC invasiveness. Methods Hsa_circ_0003288 expression was measured by real-time quantitative reverse transcriptase PCR (qRT-PCR). Luciferase reporter assays, RNA pull-down assay and fluorescence in situ hybridization (FISH) were used to determine the correlation between hsa_circ_0003288 and miR-145 and between miR-145 and PD-L1. Furthermore, ectopic overexpression and siRNA-mediated downregulation of hsa_circ_0003288, transwell assays, and in vivo studies were used to determine the function of hsa_circ_0003288 on the EMT and invasiveness of L02 and HCC cells. Results miR-145 directly targeted the PD-L1 3′-untranslated region (UTR) region, and hsa_circ_0003288 acted as a miR-145 sponge to regulate PD-L1 expression. Overexpression of hsa_circ_0003288 increased PD-L1 levels and promoted EMT, migration, and invasiveness of L02 cells. These observations were reversed after knockdown of hsa_circ_0003288 in HepG2 and Huh7 cells. Overexpression of PD-L1 rescued EMT, migration, and invasiveness of HepG2 and Huh7 cells after knockdown of hsa_circ_0003288. Furthermore, hsa_circ_0003288 knockdown reduced EMT in in vivo studies. Hsa_circ_0003288/PD-L1 axis was found to mediate the metastatic phenotypes via the PI3K/Akt pathway in HCC. Additionally, expression levels of hsa_circ_0003288 were increased and positively correlated with PD-L1 expression in HCC tissues. Conclusion Our findings demonstrated that hsa_circ_0003288 promoted EMT and invasion of HCC via the hsa_circ_0003288/miR-145/PD-L1 axis through the PI3K/Akt pathway. Targeting hsa_circ_0003288 may be a therapeutic strategy for the treatment of HCC.

show abstract

Section: Discussioncontrasting

confidence: 99%

Circular RNA hsa_circ_0003288 induces EMT and invasion by regulating hsa_circ_0003288/miR-145/PD-L1 axis in hepatocellular carcinoma

Zhang

Liang

et al. 2021

Cancer Cell Int

View full text Add to dashboard Cite

show abstract

“…This result highlights a link for miR145 and disease progression. However, the role of this miRNA in renal tissue damage is unclear, as miR145 can positively or negatively regulate fibrosis in different pathological processes [ 31 , 32 , 33 , 34 , 35 ]. In tumor cells, miR145 prevented cell invasion and EMT [ 32 , 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

Upregulation of miR145 and miR126 in EVs from Renal Cells Undergoing EMT and Urine of Diabetic Nephropathy Patients

Dimuccio

Bellucci²,

Genta³

et al. 2022

IJMS

View full text Add to dashboard Cite

Diabetic nephropathy (DN) is a severe kidney-related complication of type 1 and type 2 diabetes and the most frequent cause of end-stage kidney disease. Extracellular vesicles (EVs) present in the urine mainly derive from the cells of the nephron, thus representing an interesting tool mirroring the kidney’s physiological state. In search of the biomarkers of disease progression, we here assessed a panel of urinary EV miRNAs previously related to DN in type 2 diabetic patients stratified based on proteinuria levels. We found that during DN progression, miR145 and miR126 specifically increased in urinary EVs from diabetic patients together with albuminuria. In vitro, miRNA modulation was assessed in a model of TGF-β1-induced glomerular damage within a three-dimensional perfusion system, as well as in a model of tubular damage induced by albumin and glucose overload. Both renal tubular cells and podocytes undergoing epithelial to mesenchymal transition released EVs containing increased miR145 and miR126 levels. At the same time, miR126 levels were reduced in EVs released by glomerular endothelial cells. This work highlights a modulation of miR126 and miR145 during the progression of kidney damage in diabetes as biomarkers of epithelial to mesenchymal transition.

show abstract

“…Moreover, miR-145-5p negatively regulated SMAD5 in esophageal cancer, which in turn can activate the TGF beta signaling pathway. High expression of miR-145 decreased angiogenesis [ 51 ]; induced differentiation of macrophages by interleukin 6 (IL-6) [ 52 ]; silenced the stem cell self-renewal and pluripotency program by suppressing multiple pluripotent genes such as OCT4, SOX2, and KLF4 [ 53 ]; promoted the epithelial–mesenchymal transition by suppressing FGF10 [ 54 ]; and increased apoptosis in prostate cancer [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

“…It has been sponged by multiple circular RNAs and lncRNAs, leading to deregulation of downstream gene targets ( Figure 9 ). Previous studies have indicated that miR-145 is associated with cell apoptosis, proliferation, stem cell differentiation, angiogenesis, and metastasis [ 50 , 51 , 52 , 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

A miRNA-Based Prognostic Model to Trace Thyroid Cancer Recurrence

Toraih

Fawzy

Niu

et al. 2022

Cancers

View full text Add to dashboard Cite

Papillary thyroid carcinomas (PTCs) account for most endocrine tumors; however, screening and diagnosing the recurrence of PTC remains a clinical challenge. Using microRNA sequencing (miR-seq) to explore miRNA expression profiles in PTC tissues and adjacent normal tissues, we aimed to determine which miRNAs may be associated with PTC recurrence and metastasis. Public databases such as TCGA and GEO were utilized for data sourcing and external validation, respectively, and miR-seq results were validated using quantitative real-time PCR (qRT-PCR). We found miR-145 to be significantly downregulated in tumor tissues and blood. Deregulation was significantly related to clinicopathological features of PTC patients including tumor size, lymph node metastasis, TNM stage, and recurrence. In silico data analysis showed that miR-145 can negatively regulate multiple genes in the TC signaling pathway and was associated with cell apoptosis, proliferation, stem cell differentiation, angiogenesis, and metastasis. Taken together, the current study suggests that miR-145 may be a biomarker for PTC recurrence. Further mechanistic studies are required to uncover its cellular roles in this regard.

show abstract

MicroRNA-145 promotes the epithelial-mesenchymal transition in peritoneal dialysis-associated fibrosis by suppressing fibroblast growth factor 10

Cited by 21 publications

References 48 publications

Circular RNA hsa_circ_0003288 induces EMT and invasion by regulating hsa_circ_0003288/miR-145/PD-L1 axis in hepatocellular carcinoma

Circular RNA hsa_circ_0003288 induces EMT and invasion by regulating hsa_circ_0003288/miR-145/PD-L1 axis in hepatocellular carcinoma

Upregulation of miR145 and miR126 in EVs from Renal Cells Undergoing EMT and Urine of Diabetic Nephropathy Patients

A miRNA-Based Prognostic Model to Trace Thyroid Cancer Recurrence

Contact Info

Product

Resources

About