MicroRNA-153 acts as a prognostic marker in gastric cancer and its role in cell migration and&amp;nbsp;invasion

Zhang, Zhengliang; Sun, Jiangli; Bai, Zhenghai; Li, Haijun; He, Shicai; Chen, Rui; Che, Xiangming

doi:10.2147/ott.s78236

Cited by 36 publications

(37 citation statements)

References 23 publications

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“…miR-153 has recently been considered a crucial factor in tumor metastasis ( 12 , 15 , 20 ). However, the role of miR-153 in PDAC remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

“…MiR-153 high: value of expression > the median value; miR-153 low: value of expression < the median value. The relative expression of miR-153 was shown as the fold difference relative to U6 ( 20 ).…”

Section: Methodsmentioning

confidence: 99%

“…miRNA vectors, including miR-153 expression vector (HmiR0039-MR04), the control vector for miR-153 (CmiR0001-MR04), miR-153 inhibitor (HmiR-AN0214-AM04) and the negative control for the miR-153 inhibitor (CmiR-AN0001-AM04) were purchased from GeneCopoeia (Guangzhou, China). The cells were transfected with the vectors mentioned above using Lipofectamine 2000 according to the manufacturer’s instructions (Invitrogen) ( 20 ).…”

Section: Methodsmentioning

confidence: 99%

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MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma

et al. 2015

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Human pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with early metastasis, which leads to poor prognosis for patients. Mounting evidence suggests that microRNAs (miRNAs) act as critical factors for tumor recurrence and metastasis. miR-153 has been suggested as a novel tumor-associated miRNA, which is involved in tumor metastasis. However, the clinical significance of miR-153 and its role in PDAC remains to be investigated. The aim of the present study was to investigate the expression levels of miR-153 using RT-qPCR in human PDAC cell lines and tissues. A clinical association analysis was performed to investigate the clinical significance of miR-153. The results showed that, the relative expression of miR-153 in PDAC cells was obviously decreased as compared to that in the normal human pancreatic duct epithelial cell line. The mean expression of miR-153 in PDAC tissues was significantly reduced as compared to that in the normal pancreatic tissues. The clinical analysis revealed that a low expression of miR-153 was closely associated with poor prognostic features and shorter long-term survival of PDAC patients. Furthermore, univariate and multivariate Cox regression analyses showed that miR-153 was an independent prognostic factor for predicting survival in PDAC patients. In vitro studies demonstrated that the upregulation of miR-153 inhibited migration and invasion in MIAPaCa-2 cells. By contrast, the downregulation of miR-153 increased the number of migrated and invaded AsPC-1 cells. miR-153 inversely regulated SNAI1 abundance in MIAPaCa-2 cells. Notably, SNAI1 was identified as a direct target of miR-153 in PDAC. Furthermore, an inverse correlation between miR-153 and SNAI1 expression was observed in PDAC tissues. In conclusion, the results showed miR-153 is an independent prognostic marker for predicting survival in PDAC patients and inhibits cell migration and invasion by targeting SNAI1.

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“…miR-153 has recently been considered a crucial factor in tumor metastasis ( 12 , 15 , 20 ). However, the role of miR-153 in PDAC remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma

et al. 2015

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“… 5 – 8 In recent years, an increased number of studies have indicated that miRNAs have crucial functions in specific cellular processes such as differentiation, morphogenesis, and tumorigenesis and that miRNAs may also be considered oncogenes or tumor suppressors. 9 – 13 …”

Section: Introductionmentioning

confidence: 99%

miR-20b overexpression is predictive of poor prognosis in gastric cancer

Xue¹,

Tao²,

Zhang³

et al. 2015

OTT

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BackgroundmiR-20b has been shown to be aberrantly expressed in several tumor types. However, the clinical significance of miR-20b in the prognosis of patients with gastric cancer (GC) is poorly understood, and the exact role of miR-20b in GC remains unclear.Materials and methodsThe expression of miR-20b was detected in 102 patients with GC by a SYBR Green assay and was compared with the expression in matched adjacent normal tissue specimens. The aim of the present study was to investigate the association of the expression of miR-20b with the clinicopathological characteristics and the overall survival of patients with GC as analyzed by Kaplan–Meier analysis and Cox proportional hazards regression models.ResultsOur results showed that miR-20b expression was upregulated in GC tissue compared with normal mucosa (P=0.00). Furthermore, miR-20b expression was positively correlated with advanced lymph node metastasis (P=0.041), tumor node metastasis stage (P=0.000), and deeper and distant metastasis (P=0.031). The overall survival rate of patients with GC was significantly lower in those whose tumors expressed high levels of miR-20b mRNA compared with those whose tumors expressed low levels of miR-20b mRNA (P=0.019).ConclusionmiR-20b may serve as a potential molecular marker for the prognosis of GC.

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“…The importance of microRNAs (miRNAs) have been testified in ischemic ailments, including cerebral ischemia (Ouyang, Stary, Yang, & Giffard, 2013). MiR-153 is a concerned miRNA, which has been extensively researched in diverse illnesses (Cao, Lv, & Lv, 2015;Zhang et al, 2015). An interesting research discovered that miR-153 inhibition could guard neurons against ischemia/reperfusion injury in an oxygen-glucose deprivation and reoxygenation model through adjusting nuclear factor erythroid 2-related factor 2/heme oxygenase 1 signaling (Ji et al, 2017).…”

mentioning

confidence: 99%

Retracted: Ganoderic Acid A exerts the cytoprotection against hypoxia‐triggered impairment in PC12 cells via elevating microRNA‐153

Lou

Zhang

et al. 2019

Phytotherapy Research

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Ganoderic Acid A (GAA) is often applied for healing cardiovascular and cerebrovascular ailments, but the influences in cerebral ischemia injury are still hazy. The research delved into the functions of GAA in hypoxia‐triggered impairment in PC12 cells. PC12 cells received hypoxia management for 12 hr, and subsequently, cell viability, migration, apoptosis, and correlative protein levels were assessed. After preprocessing with GAA, above cell behaviors were monitored again. The vector of microRNA (miR)‐153 inhibitor was utilized for PC12 cell transfection to further explore the functions of miR‐153 in hypoxia‐impaired cells. Pathways of phosphatidylinositol 3‐kinase (PI3K)/protein kinase B (AKT) and mammalian target of rapamycin (mTOR) were investigated via executing western blot for uncovering the latent mechanism. Results revealed that hypoxia disposition triggered PC12 cells impairment via restraining cell viability and migration and accelerating apoptosis. However, GAA visibly mollified hypoxia‐provoked impairment in PC12 cells. Interestingly, the enhancement of miR‐153 triggered by GAA was observed in hypoxia‐impaired PC12 cells. After miR‐153 inhibitor transfection, the protective functions of GAA in hypoxia‐impaired PC12 cells were dramatically inversed. Furthermore, GAA caused PI3K/AKT and mTOR activations via enhancement of miR‐153 in hypoxia‐impaired PC12 cells. The findings evinced that GAA exhibited the protective functions in PC12 cells against hypoxia‐evoked impairment through activating PI3K/AKT and mTOR via elevating miR‐153.

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MicroRNA-153 acts as a prognostic marker in gastric cancer and its role in cell migration and invasion

Cited by 36 publications

References 23 publications

MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma

MicroRNA-153 is a prognostic marker and inhibits cell migration and invasion by targeting SNAI1 in human pancreatic ductal adenocarcinoma

miR-20b overexpression is predictive of poor prognosis in gastric cancer

Retracted: Ganoderic Acid A exerts the cytoprotection against hypoxia‐triggered impairment in PC12 cells via elevating microRNA‐153

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