microRNA-25 promotes osteosarcoma cell proliferation by targeting the cell-cycle inhibitor p27

Wang, Xiu‐Hui; Cai, Pan; Wang, Minghui; Wang, Zhe

doi:10.3892/mmr.2014.2260

Cited by 35 publications

(25 citation statements)

References 21 publications

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“…One of the 2 studies reported that miR-25 was transfected into OS cells directly [49]. This study confirmed that miR-25 was frequently over-expressed in OS, and up-regulation of miR-25 promoted cell proliferation in vitro and tumor growth in a xenograftmouse model.…”

Section: Resultssupporting

confidence: 60%

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MicroRNAs for osteosarcoma in the mouse: a meta-analysis

Chang

Yao

et al. 2016

Oncotarget

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Osteosarcoma (OS) is the most common primary malignant bone carcinoma with high morbidity that happens mainly in children and young adults. As the key components of gene-regulatory networks, microRNAs (miRNAs) control many critical pathophysiological processes, including initiation and progression of cancers. The objective of this study is to summarize and evaluate the potential of miRNAs as targets for prevention and treatment of OS in mouse models, and to explore the methodological quality of current studies. We searched PubMed, Web of Science, Embase, Wan Fang Database, VIP Database, China Knowledge Resource Integrated Database, and Chinese BioMedical since their beginning date to 10 May 2016. Two reviewers separately screened the controlled studies, which estimate the effects of miRNAs on osteosarcoma in mice. A pair-wise analysis was performed. Thirty six studies with enough randomization were selected and included in the meta-analysis. We found that blocking oncogenic or restoring decreased miRNAs in cancer cells could significantly suppress the progression of OS in vivo, as assessed by tumor volume and tumor weight. This meta-analysis suggests that miRNAs are potential therapeutic targets for OS and correction of the altered expression of miRNAs significantly suppresses the progression of OS in mouse models, however, the overall methodological quality of studies included here was low, and more animal studies with the rigourous design must be carried out before a miRNA-based treatment could be translated from animal studies to clinical trials.

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Section: Resultssupporting

confidence: 60%

“…Four of the 25 studies reported that miRNAs functions as onco-miRNAs in OS [34, 49-51]. A total of 26 mice in the intervention arm and 36 in the control arm were included.…”

Section: Resultsmentioning

confidence: 99%

MicroRNAs for osteosarcoma in the mouse: a meta-analysis

Chang

Yao

et al. 2016

Oncotarget

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“…Although several miRNAs have been found to be deregulated in osteosarcoma cells and tissues, the exact mechanism by how miRNA regulated the tumorigenesis is still not fully elucidated [31][32][33][34]. Our study showed that miR-423-5p expression was decreased in osteosarcoma tissues and cell lines.…”

Section: Discussionmentioning

confidence: 56%

miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1

Wang¹,

Peng²,

Gong³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Increasing evidences suggest that dysregulated expression of miRNAs contributes to the progression of various tumors. However, the underlying function of miR-423-5p in osteosarcoma remains unexplored. Methods: The expression of miR-423-5p and STMN1 were determined in osteosarcoma samples and cell lines via quantitative real-time PCR. Colony formation and Cell Counting Kit-8 (CCK-8) assays were performed to measure cell proliferation ability and transwell analysis was used to detect cell invasion, and dual luciferase reporter assay was perform to analysis the interaction between the miR-423-5p and STMN1. Results: The expression levels of miR-423-5p and STMN1 in the osteosarcoma tissues and cell lines were measured by qRT-PCR. Cell viability was determined using the clone formation and CCK-8 assays. A dual-luciferase reporter and Western blot were performed to stdudy the target gene of miR-423-5p. Here, we showed that miR-423-5p expression was downregulated in osteosarcoma tissues and cell lines. However, the expression of stathmin1 (STMN1) was downregulated in osteosarcoma tissues and cell lines. Moreover, STMN1 expression level was negatively correlated with the miR-423-5p expression in the osteosarcoma tissues. We identified STMN1 was a direct target gene of miR-423-5p in osteosarcoma cell. Overexpression of miR-423-5p inhibited osteosarcoma cell proliferation, colony formation and invasion. Furthermore, we demonstrated that STMN1 was involved in miR-423-5p-mediated cell behavior such as cell proliferation, colony formation and invasion in the osteosarcoma cell. Conclusion: Our present study indicated that miR-423-5p acted as a tumor suppressor gene in osteosarcoma partly through inhibiting STMN1 expression.

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“…and Wang et al . revealed that the expression of p27 was negatively regulated by miR-802 and miR-254041, thus illustrating that p27 might be regulated by several miRNAs simultaneously in OS. Together, our data indicate that miR-199a-5p as an oncogenic miRNA can remarkably promote OS progression by targeting both PIAS3 and p27.…”

Section: Discussionmentioning

confidence: 92%

MicroRNA-199a-5p promotes tumour growth by dual-targeting PIAS3 and p27 in human osteosarcoma

Wang

Guo

et al. 2017

Sci Rep

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Osteosarcoma (OS) is the most common primary bone malignancy and remains a leading cause of cancer-related deaths in adolescents. Emerging evidence indicates that microRNAs (miRNAs) are correlated with clinical and biological characteristics of OS. However, the involvement of miR-199a-5p in OS development remains unclear. In this study, we examined the function of miR-199a-5p in vitro and in vivo. The results showed that miR-199a-5p was significantly up-regulated in OS patient tissues and cells. The inhibition of miR-199a-5p led to a significant decrease in cell proliferation and tumour growth. We further demonstrated that miR-199a-5p could directly bind to the 3′UTRs of the mRNA of both PIAS3 and p27 and mediate a decrease in the protein levels of PIAS3 and p27, thereby stimulating STAT3 activation and cell cycle progression in OS cells. Rescue experiments of PIAS3 and p27 further revealed that PIAS3 and p27 were functional targets of miR-199a-5p. Moreover, enhancing the expressions of both PIAS3 and p27 using miR-199a-5p-targeted inhibitors in an OS xenograft model was shown to be a promising approach for OS clinical therapy. Our findings indicate that the pathway of miR-199a-5p targeting both PIAS3 and p27 is a possible mechanism that contributes to tumour growth in OS.

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microRNA-25 promotes osteosarcoma cell proliferation by targeting the cell-cycle inhibitor p27

Cited by 35 publications

References 21 publications

MicroRNAs for osteosarcoma in the mouse: a meta-analysis

MicroRNAs for osteosarcoma in the mouse: a meta-analysis

miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1

MicroRNA-199a-5p promotes tumour growth by dual-targeting PIAS3 and p27 in human osteosarcoma

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