MicroRNA 486 is a potentially novel target for the treatment of spinal cord injury

Jee, Min Ki; Jung, Jin Sun; Choi, Jae-Young; Jang, Jae-Won; Kang, Kyung Sun; Im, Young Jae; Kang, Seok Kwon

doi:10.1093/brain/aws047

Cited by 90 publications

(79 citation statements)

References 35 publications

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“…These results suggest that miR20a is a potential target for therapeutic intervention following SCI. Traumatic injury induces miR-486 upregulation in a murine model of spinal cord injury (72). Additionally, miR-486 is significantly increased by the accumulation of reactive oxygen species (ROS) in vitro and in vivo.…”

Section: Traumatic Injurymentioning

confidence: 99%

Neuroprotection of microRNA in neurological disorders (Review)

Wang

Cheng

et al. 2014

Biomedical Reports

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Abstract. MicroRNAs (miRNAs) are small, endogenous, non-coding RNA molecules that function as post-transcriptional regulators of gene expression by imperfect base-pairing with the 3'-untranslated regions of their target mRNAs. Altered expression of numerous miRNAs has been shown to be extensively involved in the pathogenesis of various diseases and cancers. Additionally, the specific expression of miRNAs in the nervous system has indicated that miRNAs are critical for the occurrence and development of neurological diseases. Increasing evidence has shown that specific miRNAs target the expression of particular proteins that are significant in the disease pathogenesis. Therefore, miRNA-mediated regulation may be important in the occurrence and development of neurological diseases and may function as a novel biomarker and tool for clinical therapy. In the present study, the significance of miRNAs is reviewed in a number of neurological disorders and the possibility of their use in therapeutic interventions is evaluated.

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Section: Traumatic Injurymentioning

confidence: 99%

Neuroprotection of microRNA in neurological disorders (Review)

Wang

Cheng

et al. 2014

Biomedical Reports

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“…Another study revealed that some mRNAs of inflammatory mediators, such as intercellular adhesion molecule 1 (ICAM-1), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), were potential targets of miR-181a, miR-411, miR-99a, miR-34a, miR-30c, miR-384-5p and miR-30b-5p 16, miR-486 17, which were down-regulated post-SCI, and miR-17 and miR-20 31, miR-124a 36, which were up-regulated post-SCI, and miR-124a, which was down-regulated at 1 and 7 days post-SCI 36. On the other hand, some mRNAs of anti-inflammatory mediators, such as TRAF6, annexin A1 and A2, were likely targets of miR-146a 31, 32, miR-223 31, 36, miR-221 16 and miR-1 16, 32, which were up-regulated post-SCI, and miR-1, which was down-regulated at 3 days post-SCI 31, 36.…”

Section: Introductionmentioning

confidence: 99%

microRNAs in Spinal Cord Injury: Potential Roles and Therapeutic Implications

Ning¹,

Gao²,

Liu³

et al. 2014

Int. J. Biol. Sci.

100

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microRNAs (miRNAs) are a novel class of small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. miRNAs can modulate gene expression and thus play important roles in diverse neurobiological processes, such as cell differentiation, growth, proliferation and neural activity, as well as the pathogenic processes of spinal cord injury (SCI) like inflammation, oxidation, demyelination and apoptosis. Results from animal studies have revealed the temporal alterations in the expression of a large set of miRNAs following SCI in adult rats, and the expressional changes in miRNAs following SCI is bidirectional (increase or decrease). In addition, several miRNAs have distinct roles in prognosis of SCI (protective, detrimental and varied). Taken together, the existing evidence suggests that abnormal miRNA expression following SCI contributes to the pathogenesis of SCI, and miRNAs may become potential targets for the therapy of SCI.

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“…The SCI model was established as previously described (20). Briefly, the mice were anesthetized by intraperitoneally injection with 10% chloral hydrate (30 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

Protective role of microRNA‑219‑5p inhibitor against spinal cord injury via liver receptor homolog‑1/Wnt/β‑catenin signaling pathway regulation

Shen

2018

Exp Ther Med

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Abstract. The present study aimed to investigate the role of microRNA (miR)-219-5p in spinal cord injury (SCI) and to examine the underlying molecular mechanism. SCI rat and cell models were conducted in the current study, while reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the level of miR-219-5p in the SCI mice and neurons. Bioinformatics analysis was applied to predict the target genes of miR-219-5p, and dual-luciferase reporter assay was performed to verify the prediction. In addition, MTT assay and flow cytometry were conducted to determine the cell viability and cell apoptosis of the neurons, respectively. Western blot analysis was also performed to detect the expression of associated proteins. The study results demonstrated that miR-219-5p was highly expressed in SCI mice and neurons, and directly targets liver receptor homolog-1 (LRH-1). The neuron viability was significantly reduced by SCI, however, it was recovered upon transfection with an miR-219-5p inhibitor. Neuron apoptosis was notably induced by SCI and inhibited by miR-219-5p inhibition. The LRH-1/Wnt/β-catenin signaling pathway was also inhibited by SCI, while it was significantly enhanced by the miR-219-5p inhibitor. Furthermore, LRH-1 overexpression eliminated the effects of the miR-219-5p inhibitor on SCI. In conclusion, these data indicated that the miR-219-5p inhibitor served a protective role in SCI via regulating the LRH-1/Wnt/β-catenin signaling pathway.

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MicroRNA 486 is a potentially novel target for the treatment of spinal cord injury

Cited by 90 publications

References 35 publications

Neuroprotection of microRNA in neurological disorders (Review)

Neuroprotection of microRNA in neurological disorders (Review)

microRNAs in Spinal Cord Injury: Potential Roles and Therapeutic Implications

Protective role of microRNA‑219‑5p inhibitor against spinal cord injury via liver receptor homolog‑1/Wnt/β‑catenin signaling pathway regulation

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