MicroRNA-7 Is Associated with Malignant Potential and Poor Prognosis in Human Colorectal Cancer

Abstract

Dysregulation pattern of microRNA-7 (miR-7) and the clinical significance of its expression in colorectal cancer (CRC) are unresolved. Here, we investigated the relationship between clinicopathological factors, including prognostic outcome, and miR-7 expression in CRC tissues. We analyzed miR-7 expression levels by real-time reverse transcription polymerase chain reaction in 210 colorectal tissues (196 CRCs and 14 normal mucosae from healthy volunteers). miR-7 expression levels were significantly up-regulated in CRC tissues compared to normal colorectal mucosae. Elevated miR-7 expression was significantly correlated with tumour depth, venous invasion, lymphatic invasion, lymph node metastasis, and liver metastasis. High miR-7 expression was significantly associated with poor overall survival in patients with CRC (p=0.010). Moreover, miR-7 expression was an independent prognostic factor in patients with CRC (hazard ratio=1.854, 95% confidence interval=1.016-3.540; p=0.044). miR-7 acts as an oncogene in CRC and is significantly associated with tumour progression and poor prognosis.

Regulatory network analysis of LINC00472, a long noncoding RNA downregulated by DNA hypermethylation in colorectal cancer

“…HNRNPA1 expression level was higher in low‐stage CRC compared with that of high‐stage CRC, while HNRNPH1 was downregulated in CRC . For miRNAs, all these miRNAs are upregulated in CRC such as miR‐96 and miR‐7 . These increased miRNAs may repress the expression of LINC00472 in CRC.…”

“…37 For miRNAs, all these miRNAs are upregulated in CRC such as miR-96 38 and miR-7. 39 These increased miRNAs may repress the expression of LINC00472 in CRC. The proteins or miR-NAs in the regulatory network may interact with LINC00472 and further affect the promotion and development of CRC.…”

Tissue micro-RNAs associated with colorectal cancer prognosis: a systematic review

“…A total of 32 studies demonstrated the association between the downregulation of 32 miRs and the presence of lymph node metastasis such as miR-6852 106 , miR-302c 67 , miR-466 68 , miR-340 69 , miR-944 71 , miR-30d 72 , miR-22 50 , miR-296 73 , miR-199b 47 , let-7a-5p 98 , miR-132 99 , miR-497 107 , miR-206 100 , miR-128 75 , miR-154 78 , miR-149 79 , miR-24-3p 54 , miR-15a 34 , miR-16 34, 36 , miR-193a-5p 108 , miR-100 81 , miR-133a 41 , miR-205 109 , miR-335 82 , miR-378 101 , miR-218 83 , miR-138 110 , miR-625 111 , miR-212 112 , miR-195 113 , miR-133b 37 and miR-30c 103 . Other 30 studies verified the upregulation of miR-1296 84 , miR-200c 114 , miR-21 26, 29 , miR-130a 85 , miR-7 55 , miR-96 86 , miR-1260b 115 , miR-517a 87 , miR-106b 88 , miR-320e 89 , miR-196a 57 , miR-196b 57 , miR-376a 116 , miR-720 91 , miR-494 61 , miR-630 92 , miR-181a 31 , miR-20a 62 , miR-210 93 , miR-182 43, 44 , miR-25 94 , miR-191 95 , miR-183 46 , miR-199a-3p 117 , miR-429 105 , miR-92a 96 , miR-10b 118 , miR-155 30 and miR-552 97 associated with the presence of lymph node metastasis.…”

“…MiR-181a 31–33 , miR-16 34–36 and miR-133b 37–39 were individually investigated in three studies. MiR-126 8, 40 , miR-133a 41, 42 , miR-182 43, 44 , miR-183 45, 46 , miR-199b 47, 48 , miR-22 49, 50 , miR-224 51, 52 , miR-24-3p 53, 54 , miR-7 55, 56 , were separately analyzed in two studies. Seven researches studied two miRs concomitantly 30, 34, 38, 39, 57–59 , while the others analyzed a single miR.…”

“…Two studies demonstrated the upregulation of miR-182 43, 44 associated with advanced TNM stage and others 22 studies pointed 23 different upregulated miRs associated with advanced TNM stage. In detail, miR-1296 84 , miR-888 64 , miR-21 29 , miR-130a 85 , miR-7 55 , miR-96 86 , miR-20a-5p 63 , miR-517a 87 , miR-106b 88 , miR-320e 89 , miR-592 90 , miR-196a 57 , miR-196b 57 , miR-720 91 , miR-133a 42 , miR-630 92 , miR-181a 31 , miR-210 93 , miR-25 94 , miR-191 95 , miR-92a 96 , miR-31 59 and miR-552 97 have been reported.…”