MicroRNA-95-3p serves as a contributor to cisplatin resistance in human gastric cancer cells by targeting EMP1/PI3K/AKT signaling

Ni, Qingfeng; Zhang, Yan; Tao, Ran; Li, Xiaolong; Jun, Zhu

doi:10.18632/aging.202679

Cited by 23 publications

(17 citation statements)

References 46 publications

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“…EMP1 has moved into the limelight in these tumor entities, where its expression is controversial in multiple cancer types; however, its expression and function are contradictory. A previous study has corroborated that EMP1 serves as a promising oncogene and is obviously downregulated in tumors and can enhance cell cytotoxicity in various cancer cells [ 35 ]. The underlying mechanisms of EMP1 ability in inducing ferroptosis have not been elucidated.…”

Section: Discussionmentioning

confidence: 92%

Epithelial Membrane Protein 1 Promotes Sensitivity to RSL3-Induced Ferroptosis and Intensifies Gefitinib Resistance in Head and Neck Cancer

Wang

Zhang

Yao

et al. 2022

Oxidative Medicine and Cellular Longevity

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Epithelial membrane protein (EMP1), a member of the peripheral myelin protein (PMP22) family, is involved in the development of various human malignancies. However, the expression level of EMP1 and its functional role in head and neck squamous cell carcinoma (HNSCC) remain unclear to date. Ferroptosis, a newly characterized form of regulated cell death, plays an essential role in tumorigenesis. In this study, we aimed to investigate the expression levels of EMP1 in HNSCC and normal tissues, as well as to identify the function of EMP1 in regulating ferroptosis during the progression of HNSCC. To further explore the biological function of EMP1 in vitro, transient transfection was used to overexpress EMP1 in the HNSCC cell lines Hep2 and Detroit562. Functionally, our results indicated that EMP1 overexpression could not affect the initiation of ferroptosis directly but reinforced RSL3-induced ferroptosis on HNSCC cells. Furthermore, mechanical study indicated that EMP1 mediated the ferroptosis via cell density-regulated Hippo-TAZ pathway and regulated the expression of Rac1 and NOX1. In addition, our study demonstrated that EMP1 overexpression could promote gefitinib resistance by targeting the MAPK pathway. In summary, our findings indicate that EMP1 may act as an oncogene and serve as a therapeutic target against malignant progression of HNSCC.

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Section: Discussionmentioning

confidence: 92%

Epithelial Membrane Protein 1 Promotes Sensitivity to RSL3-Induced Ferroptosis and Intensifies Gefitinib Resistance in Head and Neck Cancer

Wang

Zhang

Yao

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Interestingly, c-Myc inversely regulates the expression of miR-567, leading to the formation of a miR-567-PIK3AP1-PI3K/AKT-c-Myc feedback loop (37). Ni et al found that miR-95-3p activates the PI3K/AKT signaling pathway by upregulating the expression of p-PI3K and p-AKT, leading to the enhancement of CDDP resistance of GC (76). PTEN is a key negative regulator of the PI3K/AKT signaling pathway (121).…”

Section: Mirnas Regulate the Drug Resistance Of Gc By Targeting Signaling Pathwaysmentioning

confidence: 99%

“…Ni et al. found that miR-95-3p activates the PI3K/AKT signaling pathway by upregulating the expression of p-PI3K and p-AKT, leading to the enhancement of CDDP resistance of GC ( 76 ). PTEN is a key negative regulator of the PI3K/AKT signaling pathway ( 121 ).…”

Section: Implication Of Mirnas In Gc Drug Resistancementioning

confidence: 99%

Mechanisms of Action And Clinical Implications of MicroRNAs in the Drug Resistance of Gastric Cancer

Liu

et al. 2021

Front. Oncol.

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Gastric cancer (GC) is one of the most common malignant tumors of digestive systems worldwide, with high recurrence and mortality. Chemotherapy is still the standard treatment option for GC and can effectively improve the survival and life quality of GC patients. However, with the emergence of drug resistance, the clinical application of chemotherapeutic agents has been seriously restricted in GC patients. Although the mechanisms of drug resistance have been broadly investigated, they are still largely unknown. MicroRNAs (miRNAs) are a large group of small non-coding RNAs (ncRNAs) widely involved in the occurrence and progression of many cancer types, including GC. An increasing amount of evidence suggests that miRNAs may play crucial roles in the development of drug resistance by regulating some drug resistance-related proteins as well as gene expression. Some also exhibit great potential as novel biomarkers for predicting drug response to chemotherapy and therapeutic targets for GC patients. In this review, we systematically summarize recent advances in miRNAs and focus on their molecular mechanisms in the development of drug resistance in GC progression. We also highlight the potential of drug resistance-related miRNAs as biomarkers and therapeutic targets for GC patients.

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“…A large number of bioinformatics studies also show that miR-552-3p can be used as a biomarker for diagnosis and treatment of CRC [22]. Using RNA sequencing analysis (RNA-seq), miR-95-3p is linked to cisplatin resistance in gastric cancer, increasing the PI3K/Akt pathway [23,24]. Notably, to better understand the mechanisms of these miRNAs involved, we need further studies for their roles in CRLM.…”

Section: Construction Of Ppi and Dems-hubgenes Networkmentioning

confidence: 99%

Identifiction of Potential Regulatory Network in Colorectal with Liver Metastasis

Zhang¹,

Shen²,

Li³

et al. 2021

Preprint

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Background Liver metastasis is an important cause of death in patients with colorectal cancer(CRC). Increasing evidences indicates that microRNAs (miRNAs) are involved in the pathogenesis of colorectal with liver metastasis(CRLM). This study aims to explore potential miRNA–mRNA regulatory network. Methods The GEO database download the microarray datasets GSE56350 and GSE73178. GEO2R was used to conduct differentially expressed miRNAs (DEMs) between CRC and CRLM. The common miRNAs appeared in the two datasets. The transcription factors were predicted by FunRich and miRNet. Then, GO and KEGG pathway analysis for differentially expressed genes (DEGs) performed via DAVID. Besides, these hub genes network was used the STRING and identified by Cytoscape. Finally, the expression of the hub gene expression was used to assess by the GSE81558.Results Four DEMs were selected in the datasets. The SP1 was likely to adjust the up-regulated DEMs, however, the YY1 could regulate both up-regulated and down-regulated DEMs. The total of the 3925 genes were predicted( 3447 up-regulated DEM genes and 478 down-regulated DEM genes). These predicted genes were mainly linked to Platinum drug resistance, Cellular senescence and ErbB signaling pathway. Through the gene network construction, most of the hub genes were modulated by hsa-miR-204-5p, hsa-miR-122-5p, hsa-miR-95-3p and hsa-miR-552-3p. Among the top 20 hub genes, the expression of CREB1, RHOA and EGFR was significantly different in the GSE81558 dataset.Conclusion In this study, miRNA-mRNA in CRLM were screened between CRC patients and CRLM patients to provide a new method to predict for the pathogenesis and development of CRC.

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MicroRNA-95-3p serves as a contributor to cisplatin resistance in human gastric cancer cells by targeting EMP1/PI3K/AKT signaling

Cited by 23 publications

References 46 publications

Epithelial Membrane Protein 1 Promotes Sensitivity to RSL3-Induced Ferroptosis and Intensifies Gefitinib Resistance in Head and Neck Cancer

Epithelial Membrane Protein 1 Promotes Sensitivity to RSL3-Induced Ferroptosis and Intensifies Gefitinib Resistance in Head and Neck Cancer

Mechanisms of Action And Clinical Implications of MicroRNAs in the Drug Resistance of Gastric Cancer

Identifiction of Potential Regulatory Network in Colorectal with Liver Metastasis

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