MicroRNA, a new target for engineering new crop cultivars

Zhang, Baohong; Wang, Qinglian

doi:10.1080/21655979.2016.1141838

Cited by 30 publications

(7 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aberrant expression patterns of these small molecules, which might be stress-, genotype-, tissue-, miRNA-specific, induced by abiotic environment proposes their potential use as target for genetic improvement (Zhang, 2015 ). Latest advancements in genomics have provided the scientific society with genome wide high throughput sequence data of many important crop species which is a rich source of information about potential candidate transgenes, of which miRNA have become progressively demandable due to their important regulatory role in various phases of plant life cycle (Sun, 2012 ; Zhou and Luo, 2013 ; Zhang and Wang, 2016 ). The list of transgenic plants produced recently via altering the miRNA expression for improving plant tolerance to abiotic stresses is presented in Table 3 .…”

Section: Targeting Mirnas For Developing Abiotic Stress Tolerant Tranmentioning

confidence: 99%

“…Information regarding RNA mediated stress regulatory networks offers an innovative path for improved stress tolerance on genetic level. Recent reports have revealed that manipulation of miRNA arbitrated gene regulations can help to engineer plants for enhanced abiotic stress tolerance (Zhang and Wang, 2016 ). Due to their central role in intricate gene regulatory network, miRNAs may prove potent targets for plant improvement, with superior tolerance to abiotic stresses (Zhang and Wang, 2015 ).…”

Section: Targeting Mirnas For Developing Abiotic Stress Tolerant Tranmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

et al. 2016

View full text Add to dashboard Cite

The microRNAs (miRNAs) are small (20–24 nt) sized, non-coding, single stranded riboregulator RNAs abundant in higher organisms. Recent findings have established that plants assign miRNAs as critical post-transcriptional regulators of gene expression in sequence-specific manner to respond to numerous abiotic stresses they face during their growth cycle. These small RNAs regulate gene expression via translational inhibition. Usually, stress induced miRNAs downregulate their target mRNAs, whereas, their downregulation leads to accumulation and function of positive regulators. In the past decade, investigations were mainly aimed to identify plant miRNAs, responsive to individual or multiple environmental factors, profiling their expression patterns and recognizing their roles in stress responses and tolerance. Altered expressions of miRNAs implicated in plant growth and development have been reported in several plant species subjected to abiotic stress conditions such as drought, salinity, extreme temperatures, nutrient deprivation, and heavy metals. These findings indicate that miRNAs may hold the key as potential targets for genetic manipulations to engineer abiotic stress tolerance in crop plants. This review is aimed to provide recent updates on plant miRNAs, their biogenesis and functions, target prediction and identification, computational tools and databases available for plant miRNAs, and their roles in abiotic stress-responses and adaptive mechanisms in major crop plants. Besides, the recent case studies for overexpressing the selected miRNAs for miRNA-mediated enhanced abiotic stress tolerance of transgenic plants have been discussed.

show abstract

Section: Targeting Mirnas For Developing Abiotic Stress Tolerant Tranmentioning

confidence: 99%

Section: Targeting Mirnas For Developing Abiotic Stress Tolerant Tranmentioning

confidence: 99%

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

et al. 2016

View full text Add to dashboard Cite

show abstract

“…To use the ncRNAs' information in the improvisation of key traits in various crops, different molecular genetics-based approaches have been employed. There are some classic reports which demonstrate the effect of single miRNA manipulation for diverse traits such as increased crop yield, biomass, and stress tolerance (Zhang and Wang 2016). For instance, overexpression of evolutionarily conserved miR156 in tomato plants showed association with fruit size (Zhang et al 2016b).…”

Section: Harnessing the Regulatory Ncrnas For Crop Improvementmentioning

confidence: 99%

Regulatory non-coding RNAs: a new frontier in regulation of plant biology

Bhogireddy

Mangrauthia

Kumar

et al. 2021

Funct Integr Genomics

View full text Add to dashboard Cite

Beyond the most crucial roles of RNA molecules as a messenger, ribosomal, and transfer RNAs, the regulatory role of many non-coding RNAs (ncRNAs) in plant biology has been recognized. ncRNAs act as riboregulators by recognizing specific nucleic acid targets through homologous sequence interactions to regulate plant growth, development, and stress responses. Regulatory ncRNAs, ranging from small to long ncRNAs (lncRNAs), exert their control over a vast array of biological processes. Based on the mode of biogenesis and their function, ncRNAs evolved into different forms that include microRNAs (miRNAs), small interfering RNAs (siRNAs), miRNA variants (isomiRs), lncRNAs, circular RNAs (circRNAs), and derived ncRNAs. This article explains the different classes of ncRNAs and their role in plant development and stress responses. Furthermore, the applications of regulatory ncRNAs in crop improvement, targeting agriculturally important traits, have been discussed.

show abstract

“…It is worth mentioning the usefulness of the described tools not only in miRNA functional analyses but also as an effective approach for improving the agronomic traits in model and crop plants (reviewed in [ 181 , 182 , 183 ]).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Research Tools for the Functional Genomics of Plant miRNAs During Zygotic and Somatic Embryogenesis

Wójcik

2020

IJMS

View full text Add to dashboard Cite

During early plant embryogenesis, some of the most fundamental decisions on fate and identity are taken making it a fascinating process to study. It is no surprise that higher plant embryogenesis was intensively analysed during the last century, while somatic embryogenesis is probably the most studied regeneration model. Encoded by the MIRNA, short, single-stranded, non-coding miRNAs, are commonly present in all Eukaryotic genomes and are involved in the regulation of the gene expression during the essential developmental processes such as plant morphogenesis, hormone signaling, and developmental phase transition. During the last few years dedicated to miRNAs, analytical methods and tools have been developed, which have afforded new opportunities in functional analyses of plant miRNAs, including (i) databases for in silico analysis; (ii) miRNAs detection and expression approaches; (iii) reporter and sensor lines for a spatio-temporal analysis of the miRNA-target interactions; (iv) in situ hybridisation protocols; (v) artificial miRNAs; (vi) MIM and STTM lines to inhibit miRNA activity, and (vii) the target genes resistant to miRNA. Here, we attempted to summarise the toolbox for functional analysis of miRNAs during plant embryogenesis. In addition to characterising the described tools/methods, examples of the applications have been presented.

show abstract

MicroRNA, a new target for engineering new crop cultivars

Cited by 30 publications

References 29 publications

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants

Regulatory non-coding RNAs: a new frontier in regulation of plant biology

Research Tools for the Functional Genomics of Plant miRNAs During Zygotic and Somatic Embryogenesis

Contact Info

Product

Resources

About