MicroRNA expression profile and identification of novel microRNA biomarkers for metabolic syndrome

Abstract

The lack of efficient biomarkers is the main reason for the inaccurate early diagnosis and poor treatment outcomes of patients with metabolic syndrome (MetS). The current study aimed to identify several novel microRNA (miRNA) biomarkers for metabolic syndrome via high-throughput sequencing and comprehensive bioinformatics analysis. Through high-throughput sequencing and differentially expressed miRNA (DEM) analysis, we first identified two upregulated and 36 downregulated DEMs in the plasma samples of patients with MetS compared to the healthy volunteers. Additionally, we also predicted 379 potential target genes and subsequently carried out enrichment analysis and protein-protein interaction network analysis to investigate the signaling pathways and functions of the identified DEMs as well as the interactions between their target genes. Furthermore, we selected two upregulated and top 10 downregulated DEMs with the highest |log2FC| values as the key microRNAs, which may serve as potential biomarkers for MetS. RT-qPCR was performed to validated these result. Finally, hsa-miR-526b-5p, hsa-miR -6516-5p was identified as the novel biomarkers for MetS.

Maternal Body Mass Index Is Associated with Profile Variation in Circulating MicroRNAs at First Trimester of Pregnancy

“…Moreover, higher circulating levels of serum hsa-miR-1323 has been associated with GDM [34], hsa-miR-517-5p and hsa-miR-520a-5p were reported to be downregulated in PE, which is consistent with our results with maternal BMI [16,35]. Finally, lower circulating levels of hsa-miR-526b-5p were detected in patients with metabolic syndrome -characterized by central excess adiposity-a direction of association that is concordant with our results [36]. Through their mechanism of action, the miRNAs that we found associated with maternal BMI might potentially have a role to play in the pathophysiology of how excess weight in early gestation may lead to pregnancy complications.…”

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“…MicroRNAs (miRNAs) are a class of small RNA molecules with a single-stranded structure that act complementarily to mRNAs to control protein synthesis via the degradation and suppression of protein translation [ 1–3 , 24–27 ]. Increasing evidence has demonstrated that miRNAs play fundamental roles in almost all biological processes and serve as multifunctional mediators of cell growth and multiplication, cell differentiation, programmed cell death, and organ remodeling [ 28 ].…”

Analysis of MicroRNA Signature Differentially Expressed in Pancreatic Islet Cells Treated with Pancreatic Cancer-Derived Exosomes

“…The hsa-miR-4697-3p level, which was upregulated in Cancer in the present study, was reported to show a significant correlation with the blood glucose level and was upregulated in metabolically unhealthy subjects [ 40 ]. Hsa-miR-518e-5p, which was downregulated in Cancer in the present study, was reportedly downregulated in patients with metabolic syndrome [ 41 ]. In addition, patients who were successfully treated for DM showed the opposite trend in miRNA expression.…”