MicroRNA Expression Profile during Aphid Feeding in Chrysanthemum (Chrysanthemum morifolium)

Xia, Xiaolong; Shao, Yafeng; Jiang, Jiafu; Du, Xinping; Sheng, Liping; Chen, Fadi; Fang, Weimin; Guan, Zhiyong; Chen, Sumei

doi:10.1371/journal.pone.0143720

Cited by 34 publications

(23 citation statements)

References 45 publications

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“…In a study by Sattar et al [68], melon lines had different patterns of conserved and newly identified miRNA expression profiles during different stages of aphid herbivory. Also, Xia et al [69] found that different miRNAs were expressed in chrysanthemum during aphid feeding, with the greatest differences during early stages of aphid infestations (2-6 h of infestation). Here, we sampled 14 days after the beginning of infestation as our aim was not to study the effect of the insect, but that of the virus; the aphid-infested plants served only as a control to filter out the effect of the insect stress on the plants.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Small RNAs of Barley Genotypes Associated with Resistance to Barley Yellow Dwarf Virus

Jarošová

Singh

Chrpová

et al. 2020

Plants

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Barley yellow dwarf virus (BYDV) causes an often-devastating disease of cereals that is most effectively controlled by using plant genotypes that are resistant or tolerant to the virus. New barley lines Vir8:3 and Vir13:8, with pyramided resistance genes against different pathogens and resistance gene Ryd2 against BYDV, are currently being tested. Because microRNAs (miRNAs) are associated with antiviral plant defense, here we compared the miRNA profiles in these lines and in cultivar Wysor (carrying one resistance gene, Ryd2), with and without BYDV infection and after feeding by virus-free aphids, to determine whether the miRNA profile in the resistant variety bear similarities with the newly developed lines. The BYDV titer for each group was also determined and compared to the titer in sensitive cultivar Graciosa. Among 746 miRNAs identified in barley, 66 were known miRNAs, and 680 were novel. The expression of 73 miRNAs differed significantly after BYDV infection, including the strong, specific upregulation of novel miRNA10778 that was conserved across all the barley genotypes. This miRNA belongs to the H box and ACA box (H/ACA) snoR14 family of RNAs (Rf01280) and is associated with pseudourydilation. The expression of 48 miRNAs also differed depending on the barley genotype. The profile of miRNAs expressed in Vir8:3 and Vir13:8 in response to BYDV was similar and differed from that of Wysor. Insights into the expression patterns of miRNAs in response to BYDV in barley provided here will benefit further studies toward understanding the resistance mechanisms and developing novel strategies against virus infections.

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Section: Discussionmentioning

confidence: 99%

Analysis of Small RNAs of Barley Genotypes Associated with Resistance to Barley Yellow Dwarf Virus

Jarošová

Singh

Chrpová

et al. 2020

Plants

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“…Non-coding RNAs play essential roles in plant development and stress signaling transduction pathways [10,12]. While the application of RNA-Seq methodologies to analyse the expression of ncRNA responses to biotic stresses in model plants is well-established, similar studies in cotton and reports linking changes in ncRNA abundance with plant-herbivore interactions are more limited [23,25,[32][33][34]. Despite the widespread use of traditional chemical-based strategies for whitefly control in cotton, epidemics remain prevalent worldwide [35].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Cotton miRNAs during Whitefly Infestation Offers New Insights into Plant-Herbivore Interaction

Li¹,

Hull²,

Liang³

et al. 2019

Preprint

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Although the regulatory function of miRNAs and their targets have been characterized in model plants, a possible underlying role in the cotton response to herbivore infestation has not been determined. To investigate this, we performed small RNA and degradome sequencing between resistant and susceptible cotton cultivar following infestation with the generalist herbivore whitefly. In total, 260 miRNA families and 241 targets were identified. Quantitative-PCR analysis revealed that several miRNAs and their corresponding targets exhibited dynamic spatio-temporal expression patterns. Moreover, 17 miRNA precursors were generated from 29 long intergenic non-coding RNA (lincRNA) transcripts. Genome-wide analysis also led to the identification of 85 phased small interfering RNA (phasiRNA) loci. Among these, nine PHAS genes were triggered by miR167, miR390, miR482a, and two novel miRNAs, including those encoding a leucine-rich repeat (LRR) disease resistance protein, an auxin response factor (ARF) and MYB transcription factors. Through combined modeling and experimental data, we explored and expanded the miR390-tasiARF cascade during the cotton response to whitefly. Virus-induced gene silencing (VIGS) of ARF8 in whitefly-resistant cotton plants increased auxin and jasmonic acid (JA) accumulation, resulting in an increased tolerance to whitefly infestation. These results highlight the provides a useful transcriptomic resource for plant-herbivore interaction.

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“…특히 miRNA의 target mRNA가 대부분이 전사조절인 자로 하위유전자의 발현에도 영향을 주기 때문에 그 영향 력이 매우 커서 국화에서도 NGS를 활용하여 miRNA가 전 게놈 수준에서 분석되었다 (Xia et al 2015;Zhang et al 2015). 원연교잡 후에 확보된 3종류의 배에서 228개의 miRNA가 동정되었으며, 각각의 배에서 특이적으로 발현되는 miRNA 도 규명되었다 .…”

Section: 장일(Long-day) 처리 중인 영양생장점(Vegetative Buds) 단일 을 시작한 후 화아분화가 unclassified

“…한편, 진딧물 혹은 물리적 상처가 가해진 실험구와 대조구에서 miRNA를 분석하여 다 른 종에서도 잘 보존된 303개의 miRNA와 234개의 신규 miRNA를 발견하여 최종 537개의 miRNA가 확보되었다. 특히 기능이 잘 알려진 miRNA 중에서 miR159a, miR160a, miR393a 가 진딧물의 피해에 식물이 특이적으로 반응하였다 (Xia et al 2015). 두 논문에서는 miRNA의 target mRNA도 일부 보고 하였다.…”

Section: 장일(Long-day) 처리 중인 영양생장점(Vegetative Buds) 단일 을 시작한 후 화아분화가 unclassified

Current status and prospects of chrysanthemum genomics

et al. 2016

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Chrysanthemum is one of the top floriculture species with ornamental and medicinal value. Although chrysanthemum breeding program has contributed to the development of various cultivars so far, it needs to be advanced from the traditional phenotype-based selection to marker-assisted selection (molecular breeding) as shown in major cereal and vegetable crops. Molecular breeding relies on trait-linked molecular markers identified from genetic, molecular, and genomic studies. However, these studies in chrysanthemum are significantly hampered by the reproductive, genetic, and genomic properties of chrysanthemum such as self-incompatibility, inbreeding depression, allohexaploid, heterozygosity, and gigantic genome size. Nevertheless, several genetic studies have constructed genetic linkage maps and identified molecular markers linked to important traits of flower, leaf, and plant architecture. With progress in sequencing technology, chrysanthemum transcriptome has been sequenced to construct reference gene set and identify genes responsible for developments or induced by biotic or abiotic stresses. Recently, a genome sequencing project has been launched on a diploid wild Chrysanthemum species. The massive sequencing information would serve as fundamental resources for molecular breeding of chrysanthemum. In this review, we summarized the current status of molecular genetics and genomics in chrysanthemum and briefly discussed future prospects.

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MicroRNA Expression Profile during Aphid Feeding in Chrysanthemum (Chrysanthemum morifolium)

Cited by 34 publications

References 45 publications

Analysis of Small RNAs of Barley Genotypes Associated with Resistance to Barley Yellow Dwarf Virus

Analysis of Small RNAs of Barley Genotypes Associated with Resistance to Barley Yellow Dwarf Virus

Genome-Wide Analysis of Cotton miRNAs during Whitefly Infestation Offers New Insights into Plant-Herbivore Interaction

Current status and prospects of chrysanthemum genomics

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