MicroRNA expression profiles and networks in CXCL12-stimulated human endometrial stromal cells

Abstract

The chemokine stromal cell-derived factor-1 (C-X-C motif chemokine ligand 12; CXCL12) is important in the recruitment of leukocytes to the peritoneal cavity and the regulation of endometriotic tissue growth in endometriosis patients. However, the alterations in microRNA (miRNA) expression induced by CXCL12 remain to be fully elucidated. The present study evaluated key miRNAs in CXCL12-stimulated endometrial stromal cells (ESCs), and investigated the underlying cellular regulatory mechanisms of CXCL12 in endometriosis by building networks between miRNAs, genes and gene ontologies (GOs). Differential expression of miRNAs and mRNAs induced by CXCL12 stimulation in ESCs was measured using miRNA and gene chips, and it was observed that 35 miRNAs and 1,671 mRNAs were differentially expressed. Using potential target genes of the 35 miRNAs, intersections of these genes were examined and 63 intersection genes were identified. A total of 39 GOs were obtained for these intersection genes, based on information from GO databases, including immune cell chemoattractants, inflammatory and immune responses, and pathological processes of endometriotic lesions in endometriosis. In addition, miRNA-gene networks were built according to the GO and Kyoto Encyclopedia of Genes and Genomes databases. The present study, to the best of our knowledge, provides the most complete miRNAome and mRNAome profiles, and the most detailed investigation of the underlying cellular regulatory mechanisms, of the effects of CXCL12 in endometriosis. These results may facilitate the complete elucidation of the role of CXCL12 in endometriosis, and its underlying epigenetic mechanisms.

SH3BGRL2 inhibits growth and metastasis in clear cell renal cell carcinoma via activating hippo/TEAD1-Twist1 pathway

“…This genomic region is associated with several solid tumors, such as prostate cancer [42], hepatocellular carcinoma [43] and breast cancer [44]. Previous studies showed that SH3BGRL2 was downregulated in esophageal squamous cell carcinoma [14], anaplastic thyroid carcinoma [45], ovarian chemotherapy resistance cells [15] and endometrial stromal cells [46]. Our study also found SH3BGRL2 was downexpression in ccRCC tumor than adjacent normal tissues.…”

Mesenchymal Stromal Cells Are More ImmunosuppressiveIn VitroIf They Are Derived from Endometriotic Lesions than from Eutopic Endometrium

“…Proteins, not genes, bestow cellular function, and therefore ESC cyst seem to have a more immunostimulatory phenotype than ESC endo [ 37 ]. CXCL12 is a ligand of the C-X-C chemokine receptor type (CXCR) 4, and through it, ESC cyst may further increase levels of inflammation in the pelvic cavity by recruiting CXCR4-positive immune cells [ 38 ]. Interestingly, ESC endo express CXCR4 and may hence be recruited to ectopic lesions by CXCL12, which may also possess nonimmune functions in endometriosis, such as promotion of tissue repair, angiogenesis, migration, invasion, and suppression of apoptosis [ 1 , 38 , 39 ], processes proposed to be involved in growth of ectopic lesions.…”

Suppression of MicroRNA-144 Promotes CXCR4 and CXCL12 Expression and Downregulates Apoptosis in Ovarian Cancer Cells

“…CXCR4/CXCL12 pathway and related molecular mechanisms have important roles to play in biological aggressiveness in metastasis and have therapeutic target for epithelial ovarian carcinoma (Jiang et al, 2006;Oda et al, 2007;Sekiya et al, 2012;. Certain studies have investigated the role of miRNA expression regulation and interaction with chemokine family as CXCR4/CXCL12 in healthy and cancer cells (Rhodes et al, 2011;Shen et al, 2014;Mei et al, 2017). These studies suggest that CXCR4/CXCL12 signaling stimulated tumorigenesis via microRNA regulation.…”