MicroRNA in sperm from Duroc, Landrace and Yorkshire boars

Kasimanickam, Vanmathy; Kastelic, John P.

doi:10.1038/srep32954

Cited by 19 publications

(24 citation statements)

References 35 publications

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“…The sperm miRNA has been reported most extensively and identified in all mammal species examined, including humans, mice, rats, rabbits, bulls, pigs and stallions (Amanai et al 2006, Cornwall 2009, Krawetz et al 2011, Das et al 2013, Stowe et al 2014, Kasimanickam & Kastelic 2016. Recently, breed-specific differential expression of miRNAs in the pig sperm was detected using the real-time PCR (Kasimanickam & Kastelic 2016). We identified 148 and 321 miRNAs in pig ejaculated sperm and epididymal sperm respectively.…”

Section: Discussionmentioning

confidence: 90%

“…In addition to mRNAs, the diversity of the population of the sperm RNAs has now been extended to several non-coding RNAs, including miRNAs, piRNAs (Amanai et al 2006, Krawetz et al 2011, Pantano et al 2015, endo-siRNAs , small RNAs derived from tRNA, rRNA, snRNA and snoRNA (Peng et al 2012) and lncRNA . The sperm miRNA has been reported most extensively and identified in all mammal species examined, including humans, mice, rats, rabbits, bulls, pigs and stallions (Amanai et al 2006, Cornwall 2009, Krawetz et al 2011, Das et al 2013, Stowe et al 2014, Kasimanickam & Kastelic 2016. Recently, breed-specific differential expression of miRNAs in the pig sperm was detected using the real-time PCR (Kasimanickam & Kastelic 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The miRNA family is the best Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796] www.reproduction-online.org characterized of the small RNA species in sperm. They were first identified in humans (Ostermeier et al 2005), followed by confirmation of their presence in mice (Amanai et al 2006) and have now been detected in various mammals, including porcine (Curry et al 2011, Kasimanickam & Kastelic 2016, bulls (Stowe et al 2014), stallions (Das et al 2013), rats and rabbits . It has been demonstrated that mature miRNAs that are carried into the zygote by sperm during the fertilization are relatively rare compared with oocyte miRNAs (Yang et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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The similarities and differences of small RNAs in seminal plasma, epididymal sperm and ejaculated sperm remain largely undefined. We conducted a systematic comparative analysis of small RNA profiles in pig ejaculated sperm, epididymal sperm and seminal plasma and found that the diversity distribution of small RNA species was generally similar, whereas the abundance of small RNAs is dramatically different across the three libraries; miRNAs and small RNAs derived from rRNA, tRNA, small nuclear RNA, 7SK RNA, NRON RNA and cis-regulatory RNA were enriched in the three libraries, but piRNA was absent. A large population of small RNAs from ejaculated sperm are ejaculated sperm specific, and only 8-30% of small RNAs overlapped with those of epididymal sperm or seminal plasma and a small proportion (5-18%) of small RNAs were shared in the three libraries, suggesting that, in addition to the testes, sperm RNAs may also originate from seminal plasma, epididymis as well as other resources. Most miRNAs were co-distributed but differentially expressed across the three libraries, with epididymal sperm exhibiting the highest abundance, followed by ejaculated sperm and seminal plasma. The prediction of target genes of the top 10 highly expressed miRNAs across the three libraries revealed that these miRNAs may be involved in spermatogenesis, zygote development and the interaction between the environment and animals. Our study provides the first description of the similarities and differences of small RNA profiles in ejaculated sperm, epididymal sperm and seminal plasma and indicates that sperm RNA may have origins other than the testes.Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796]

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

View full text Add to dashboard Cite

show abstract

“…Interestingly the results from that study have shown 47%-52% of the miRNAs that has been detected in the wild type have been dysregulated in DICER and Drosha knockout sperm groups [87] indicating active transcription of miRNAs during spermatogenesis. Moreover, the importance of miRNAs in sperm synthesis and male infertility has been described by analyzing miRNA expression in sperm cells derived from prepubertally hemicastrated Yorkshire boars [88], Landrace and Duroc sperm [89]. On the other hand, altered expression of certain miRNAs could be associated with poor sperm quality and male infertility.…”

Section: The Role Of Mirnas In Male Gametogenesismentioning

confidence: 99%

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

Salilew‐Wondim

Gebremedhn

Hoelker

et al. 2020

IJMS

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The genetic codes inscribed during two key developmental processes, namely gametogenesis and embryogenesis, are believed to determine subsequent development and survival of adult life. Once the embryo is formed, its further development mainly depends on its intrinsic characteristics, maternal environment (the endometrial receptivity), and the embryo–maternal interactions established during each phase of development. These developmental processes are under strict genetic regulation that could be manifested temporally and spatially depending on the physiological and developmental status of the cell. MicroRNAs (miRNAs), one of the small non-coding classes of RNAs, approximately 19–22 nucleotides in length, are one of the candidates for post-transcriptional developmental regulators. These tiny non-coding RNAs are expressed in ovarian tissue, granulosa cells, testis, oocytes, follicular fluid, and embryos and are implicated in diverse biological processes such as cell-to-cell communication. Moreover, accumulated evidences have also highlighted that miRNAs can be released into the extracellular environment through different mechanisms facilitating intercellular communication. Therefore, understanding miRNAs mediated regulatory mechanisms during gametogenesis and embryogenesis provides further insights about the molecular mechanisms underlying oocyte/sperm formation, early embryo development, and implantation. Thus, this review highlights the role of miRNAs in mammalian gametogenesis and embryogenesis and summarizes recent findings about miRNA-mediated post-transcriptional regulatory mechanisms occurring during early mammalian development.

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“…As a class of endogenous small (~22 nucleotides) noncoding RNAs, miRNAs mediate post-transcriptional gene expression in animals and ne-tune the expression of approximately 30% of all mammalian protein-coding genes [4][5][6]. In addition, miRNAs regulate gene expressions not only at post-transcriptional levels but also at the transcriptional level by RNA-RNA interactions [7].…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

Ding

Liu

Zhou

et al. 2020

Preprint

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Background: MicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis. Results: In this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270 days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-423-5p, ssc-mir-34c, ssc-mir-107, ssc-165 mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 ( PLCβ1) gene was verified to be a target of ssc-mir-423-5p . Conclusions: This study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars.

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MicroRNA in sperm from Duroc, Landrace and Yorkshire boars

Cited by 19 publications

References 35 publications

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation

Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

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