MicroRNA values in children with rheumatic carditis: a preliminary study

Abstract

MicroRNAs (miRNAs) are fine regulators of gene expression which participate in the regulation of almost every phase of cell physiology, including development of immune cells and adjustment of immune response. In the studies with in vitro/in vivo model systems, specific miRNAs are revealed to have various roles in cardiovascular development and physiological functions. Furthermore, some studies have been done to understand the role of miRNAs about myocarditis, heart failure and coronary artery diseases. miRNAs crucial role in the pathogenesis of other rheumatic diseases have been investigated, however rheumatic carditis was not studied. The aim of this study is to assess values of miRNAs in children with rheumatic carditis and compare them with healthy children. This study included 36 children with rheumatic carditis (mean aged 12.1 ± 2.1 years) and age-gender matched 35 healthy controls (mean aged 11.1 ± 2.3 years). Conventional echocardiography was performed to all subjects. Using real-time polymerase chain reaction, the expression of some miRNAs (hsamiR-16-5p, hsa-miR-221-3p, hsa-miR-223-3p, hsa-miR-10a-5p, hsa-miR-24-3p, hsamiR-92a-3p, hsa-iR-320a, hsa-miR-21-5p, hsa-miR-155-5p, hsa-miR-132-3p, hsamiR-146a-5p, hsa-miR-499a-5p, hsa-miR-1, hsa-miR-125, hsa-miR-196a-5p, hsa-miR-130b-3p, hsa-miR-133b, hsa-miR150-5p,hsa-miR-204-5p, hsa-miR-203a) were analyzed. hsa-miR-16-5p(-1.46 fold, p < 0.01), hsa-miR-223-3p(-1.46 fold, p < 0.01), and hsa-miR-92a-3p(-1.27 fold, p < 0.05) expressions in the patients were lower than those of controls, whereas other examined miRNAs did not differently express between the groups. Results of the study demonstrated that significant downregulation of hsa-miR-16-5p, hsa-miR-223-3p and hsa-miR-92a-3p in children with rheumatic carditis. Since, this is the first study in children with rheumatic carditis, further studies are needed for lightening whether these miRNAs might be helpful as biomarkers.

Overexpression of MicroRNA-16 Alleviates Atherosclerosis by Inhibition of Inflammatory Pathways

“…Adhikari et al [ 19 ] also provided evidence that miR-16 was decreased in the circulation of end-stage heart failure patients. The study of Gumus et al [ 20 ] demonstrated that plasma hsa-miR-16-5p was 1.46-fold lower expressed in the patients with rheumatic carditis than that of controls. In line with these studies, we also validated miR-16 was downregulated in the plasma and PBMCs of CAD patients.…”

MiR‐16, as a potential NF‐κB‐related miRNA, exerts anti‐inflammatory effects on LPS‐induced myocarditis via mediating CD40 expression: A preliminary study

“…It has previously been observed that inhibition of miR‐16 defends against acute myocardial infarction in rats . Recent literature has shown that miR‐16‐5p is visibly downregulated in children with rheumatic carditis . In the analysis of He et al, miR‐16 played an important role in hypertensive heart diseases.…”

The lncRNA MEG3/miR‐16‐5p/VGLL4 regulatory axis is involved in etoposide‐induced senescence of tumor cells

“…Therefore, we focused on the role of miR‐16‐5p in the regulation of senescence via lncRNA MEG3. Previous studies have demonstrated that miR‐16‐5p correlates with multiple pathophysiological processes, including tumors, 49,50 nerve injury, 51 rheumatic carditis, 52 diabetic nephropathy 51 and longevity 29 . In addition, overexpression of miR‐16‐5p reduced p21 and p16 expression and the number of SA‐β‐gal staining cells, whereas down‐regulation of miR‐16‐5p contributed to the senescence phenotype, suggesting that miR‐16‐5p could regulate the senescence process of tumor cells.…”