MicroRNAs and their role for T stage determination and lymph node metastasis in early colon carcinoma

Rammer, Melanie; Webersinke, Gerald; Haitchi-Petnehazy, Sophie; Maier, Eva; Hackl, Hubert; Charoentong, Pornpimol; Malli, Theodora; Steinmair, Maria; Petzer, Andreas; Rumpold, Holger

doi:10.1007/s10585-017-9863-9

Cited by 14 publications

(6 citation statements)

References 87 publications

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“…However, in glioma 24 and leukemia 25 , the expression of miR-152-3p was reported to be elevated in cancer tissue and cells, which is significantly higher than that in normal tissue and cells. Interestingly, there is a study revealing that miR-152-3p is differentially expressed in different T_stages (T1-T4) of colon cancer, with increased expression in T2-T4 and relatively higher expression in T2 and T4 26 . In this study, bioinformatics analysis and qRT-PCR revealed that miR-152-3p was markedly elevated in colon cancer tissue and cells, and silencing miR-152-3p led to decreased cell proliferation but increased cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

miR-152-3p Affects the Progression of Colon Cancer via the KLF4/IFITM3 Axis

Zhu¹,

Shen²,

Man³

et al. 2020

Computational and Mathematical Methods in Medicine

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Objective. The purpose of this study was to investigate the relationship between miR-152-3p and the KLF4/IFITM3 axis, thereby revealing the mechanism underlying colon cancer occurrence and development, consequently providing a promising target for colon cancer treatment. Methods. Bioinformatics methods were implemented to analyze the differential expression of miRNAs and mRNAs in colon cancer, confirm the target miRNA, and predict the downstream targeted mRNAs. qRT-PCR and Western blot were performed to detect the expression of miR-152-3p, KLF4, and IFITM3. CCK-8 and colony formation assays were conducted for the assessment of cell proliferation, and flow cytometry was carried out for the detection of cell apoptosis. Finally, dual-luciferase reporter gene assay was employed to verify the targeting relationship between miR-152-3p and KLF4. Results. miR-152-3p was highly expressed in colon cancer cells, whereas KLF4 was poorly expressed. Dual-luciferase assay verified that miR-152-3p targeted to bind to KLF4 and suppressed its expression. Moreover, silencing miR-152-3p or overexpressing KLF4 was found to downregulate IFITM3, thereby inhibiting cell proliferation and potentiating cell apoptosis. In rescue experiments, we found that miR-152-3p deficiency decreased the expression of IFITM3 and weakened cancer cell proliferation, and such effects were restored when miR-152-3p and KLF4 were silenced simultaneously. Conclusion. In sum, we discovered that miR-152-3p can affect the pathogenesis of colon cancer via the KLF4/IFITM3 axis.

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Section: Discussionmentioning

confidence: 99%

miR-152-3p Affects the Progression of Colon Cancer via the KLF4/IFITM3 Axis

Zhu¹,

Shen²,

Man³

et al. 2020

Computational and Mathematical Methods in Medicine

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“…miR-548c-5p, miR-548i and miR-548am-5p were identified as the top three of 26 differentially expressed miRNAs with regard to lymph node metastasis [53]. But some other studies report no significant correlations of miRNA-expression patterns with CRC tumour stage by a miRNA microarray analysis [54]. The difference in conclusions is probably due to different detection approaches and tumour sample heterogeneity.…”

Section: Mirnas For Crc Diagnosis Treatment and Recurrence Predictionmentioning

confidence: 99%

Emerging microRNA biomarkers for colorectal cancer diagnosis and prognosis

et al. 2019

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MicroRNAs (miRNAs) are one abundant class of small, endogenous non-coding RNAs, which regulate various biological processes by inhibiting expression of target genes. miRNAs have important functional roles in carcinogenesis and development of colorectal cancer (CRC), and emerging evidence has indicated the feasibility of miRNAs as robust cancer biomarkers. This review summarizes the progress in miRNA-related research, including study of its oncogene or tumour-suppressor roles and the advantages of miRNA biomarkers for CRC diagnosis, treatment and recurrence prediction. Along with analytical technique improvements in miRNA research, use of the emerging extracellular miRNAs is feasible for CRC diagnosis and prognosis.

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“…Furthermore, the incidence of colon cancer continues to increase among persons under the age of 50, and the risk rises with age. The advancements in cancer detection and therapy, however, have not brought substantial increment in survival time of colon cancer patients [ 3 , 4 ]. It is imperative to explore useful biomarkers to improve the colon cancer therapy [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Overexpressed lncRNA LINC00893 Suppresses Progression of Colon Cancer by Binding with miR-146b-3p to Upregulate PRSS8

Zhu

Jiang

Hui

et al. 2022

Journal of Oncology

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Background. Long noncoding RNAs (lncRNAs) play a significant role in the progression and metastasis of various cancers. LINC00893 has been reported to exert antitumor effect on various cancers such as gastric cancer and thyroid cancer. Bioinformatics analysis also predicted that LINC00893 was downregulated in colon cancer. However, the clinical significance and regulating mechanism of LINC00893 in colon cancer remain unknown. Methods. Expression of LINC00893, miR-146b-3p, and PRSS8 was detected in colon cancer tissues and adjacent nontumor tissues by RT-qPCR, and clinical significance was analyzed by receiver operating characteristic curve. The regulatory mechanism of LINC00893, miR-146b-3p, and PRSS8 was investigated by dual luciferase reporter and RNA pull-down assays. Proliferation, migration, invasion, and apoptosis were measured in HCT116 and SW620 cells by MTT, EdU staining, wound healing, Transwell, TUNEL, and flow-cytometry assays. Moreover, the effect of LINC00893 on colon cancer progression was further evaluated in tumor-bearing mice. Results. LINC00893 and PRSS8 were significantly downregulated, while miR-146b-3p was upregulated in colon cancer tissues compared to control group. LINC00893, miR-146b-3p, and PRSS8 had significant diagnostic value with area under curve of 0.9383, 0.7300, and 0.9644, respectively. Overexpressed LINC00893 or silenced miR-146b-3p suppressed the proliferation, migration, and invasion while promoting apoptosis in colon cancer cells (HCT116, SW620). Moreover, miR-146b-3p overexpression reversed the inhibitory effect of LINC00893, while PRSS8 knockdown rescued the suppressive effect of miR-146b-3p inhibitor on malignant cell behaviors in colon cancer. Furthermore, the tumor growth in mice was significantly reduced by LINC00893 overexpression. Conclusion. LINC00893 overexpression suppressed the progression of colon cancer by binding with miR-146b-3p to upregulate PRSS8. LINC00893 and its downstream molecules miR-146b-3p and PRSS8 may serve as novel biomarkers and therapeutic targets of colon cancer, providing new treatment options and research approaches towards colon cancer.

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MicroRNAs and their role for T stage determination and lymph node metastasis in early colon carcinoma

Cited by 14 publications

References 87 publications

miR-152-3p Affects the Progression of Colon Cancer via the KLF4/IFITM3 Axis

miR-152-3p Affects the Progression of Colon Cancer via the KLF4/IFITM3 Axis

Emerging microRNA biomarkers for colorectal cancer diagnosis and prognosis

Overexpressed lncRNA LINC00893 Suppresses Progression of Colon Cancer by Binding with miR-146b-3p to Upregulate PRSS8

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