MicroRNAs in Osteosarcomagenesis

Kafchinski, Lisa; Jones, Kevin B.

doi:10.1007/978-3-319-04843-7_6

Cited by 17 publications

(12 citation statements)

References 54 publications

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“…Previous studies have shown that multiple miRNAs are differentially expressed in human cancers, including osteosarcoma [6,7,8], providing new insights into the complex genetic mechanisms of cancer progression. For instance, miR-16 was shown to inhibit cell proliferation by targeting IGF1R and the Raf1-MEK1/2-ERK1/2 pathway in osteosarcoma [9], while miR-802 promoted osteosarcoma cell proliferation by down-regulation of cell-cycle inhibitor, p27 [10].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

Luο

Tang

Gao

et al. 2014

Cell Physiol Biochem

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Background: Multiple MicroRNAs (miRNAs) have been identified in the development and progression of osteosarcoma. However, the expression and roles of miR-212 in osteosarcoma remain largely undefined. Methods: Real-time PCR assays were used to detect the expression of miR-212 in human osteosarcoma tissues. MiR-212 mimics were introduced into MG63 and U2OS cells. Bioinformatic prediction was used to identify the potential targets of miR-212. Protein expression analysis, luciferase assays and rescue assays were used to confirm the substrate of miR-212. Results: miR-212 was significantly down-regulated in human osteosarcoma tissues, compared with adjacent normal tissues. Introduction of miR-212 mimics into MG63 and U2OS cells inhibited cell proliferation and invasion. Besides, miR-212 overexpression could also inhibit tumor growth in the nude mice. Additionally, bioinformatic prediction suggested that the sex-determining region Y-box 4 (Sox4) is a target gene of miR-212. Sox4 inhibition phenocopied the roles of miR-212, while restored expression of Sox4 dampened miR-212-mediated suppression of tumor progression. Conclusion: The miR-212/Sox4 interaction plays an important role of in the osteosarcoma progression.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

Luο

Tang

Gao

et al. 2014

Cell Physiol Biochem

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“…Accordingly, the exogenous overexpression of miR-150 may decrease the metastasis of OS through downregulating the expression of Ezrin (30). Furthermore, with an increase in exogenous miR-150, the inhibition of Ezrin expression secreted by F5M2 cells becomes more apparent (36). Thus, there may be a dependent association between the inhibition of Ezrin expression and the dosage of exogenous miR-150 in F5M2 cells.…”

Section: A B Cmentioning

confidence: 99%

MicroRNA-150 upregulation reduces osteosarcoma cell invasion and metastasis by downregulating Ezrin

Zhan

Zhang

et al. 2016

Oncology Letters

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Abstract. The present study aimed to investigate the effect of microRNA-150 (miRNA/miR-150) in osteosarcoma (OS) cell invasion and metastasis by the regulation of Ezrin. To compare the differences in the expression of miR-150 and Ezrin, cell models of OS metastasis were established by exogenous transfection of miR-150 on the basis of different expression levels of miR-150. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to estimate these expression levels. Ezrin expression was detected by western blot assay. Methylthiazolyldiphenyl-tetrazolium bromide assay was performed to determine cells proliferation. Cell invasion and migration were measured in vitro by Transwell migration assays. Detection of apoptosis adopted flow cytometry. The results of RT-qPCR showed that the miR-150 expression in OS F5M2 cells was significantly increased following exogenous transfection of miR-150 mimics, and the expression of miR-150 was positively correlated with the concentration of the miR-150 mimics. Western blot assay indicated that the Ezrin expression in the F5M2 cells was decreased with the exogenous overexpression of miR-150. Additionally, Transwell assays revealed that the overexpression of miR-150 significantly suppressed the invasion and metastasis ability of the F5M2 cells. miR-150 upregulation may reduce OS cell invasion and metastasis by downregulating the expression of Ezrin.

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“…They are approximately~22 nucleotides in length and are expressed in a tissue-or cell-specific manner (Lagos-Quintana et al, 2002). Among them, a set of miRNAs have been confirmed to play fundamental roles in gene regulation of various orthopedic diseases, such as bone tumors (Kafchinski and Jones, 2014), osteoarthritis (Miyaki and Asahara, 2012), and rheumatoid arthritis (Furer et al, 2010). Although several miRNAs have been independently reported (Yamasaki et al, 2012;Sun et al, 2014;Jia et al, 2014), and one preliminary review discussing circulating microRNAs in ONFH has been written (Wang, X. et al, 2014;Wang, Y. et al, 2014), the miRNA expression profile of ONFH remains unclear.…”

Section: Introductionmentioning

confidence: 98%

Analysis of altered microRNA expression profile in the reparative interface of the femoral head with osteonecrosis

Hou

Roemeling

Gao

et al. 2015

Experimental and Molecular Pathology

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MicroRNAs in Osteosarcomagenesis

Cited by 17 publications

References 54 publications

MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

MicroRNA-150 upregulation reduces osteosarcoma cell invasion and metastasis by downregulating Ezrin

Analysis of altered microRNA expression profile in the reparative interface of the femoral head with osteonecrosis

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