MicroRNAs influence and longevity

Eshkoor, Sima Ataollahi; Ghodsian, Nooshin; Akhtari-Zavare, Mehrnoosh

doi:10.1186/s43042-022-00316-7

Cited by 7 publications

(4 citation statements)

References 179 publications

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“…MiRNAs are key modulators in maintaining tissue homeostasis by post-transcriptionally regulating gene expression. Thus, an imbalance in their expression has been associated with disease progression and aging-related cellular processes including senescence 44,45 . Here, we aimed to characterize miRNA gene transcription in single-cell datasets to study cell-type specific miRNA expression.…”

Section: Discussionmentioning

confidence: 99%

Cell-type-specific characterization of miRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution

de Sande,

Turunen,

Bouvy-Liivrand

et al. 2023

Preprint

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MicroRNAs (miRNAs) are a class of regulatory non-coding RNAs that finetune cellular functions by modulating the stability and abundance of their target mRNAs, thereby contributing to regulation of tissue homeostasis. MiRNA genes are transcribed similarly to protein-coding genes and recent studies have enabled their annotation and quantification genome-wide from bulk nascent transcriptomes. Here, we developed an approach to quantify and integrate miRNA gene signatures into single-cell studies. To characterize miRNA gene expression dynamics, we first compared the suitability of droplet and plate-based single-cell RNA-sequencing (scRNA-seq) platforms using the matched datasets provided by the Tabula Muris Senis and Tabula Sapiens consortiums. We found high concordance between the platforms and with cell type-specific bulk expression data. Based on the comprehensive aging profiles, our analysis comparing spleen immune cells between young and old mice revealed a concordant regulation of miRNAs involved in senescence and inflammatory pathways in multiple immune cell types, including up-regulation of mmu-mir-146a, mmu-mir-101a and mmu-mir-30 family genes. To study the aberrant regulation of immune cell homeostasis and tissue inflammation that pre-dispose to aging-related disease development, we collected transcriptome profiles from atherosclerosis development in LDLR-/-ApoB100/100mice. We found an elevated myeloid cell proportion in the adipose tissue and further characterized the cell subtypes based on reproducible transcriptome clusters. We then compared miRNA gene expression in early versus late disease and upon inflammatory challenge to monitor different stages during disease progression. At atherosclerotic stage, pro-inflammatory mmu-mir-511 expression increased in several macrophage subtypes, while immunosuppressive mmu-mir-23b∼mir-24-2∼mir-27b up-regulation was specific to Trem2+ lipid-associated macrophages. The infiltrating monocytes up-regulated mmu-mir-1938 and mmu-mir-22 expression and in classical monocytes maturation further increased mmu-mir-221∼222, mmu-mir-511 and mmu-mir-155 expression. To validate that these changes detected from single cell profiles represent miRNA gene transcriptional regulation, we used nascent transcriptomics data fromex vivomacrophage cultures with pro-inflammatory stimulation, confirming both rapid and long-lasting transcriptional activation of the miRNA loci studied. Collectively, our work enables integrating miRNA gene analysis to current single cell genomics pipelines and facilitates characterization of miRNA regulatory networks during aging and disease development.

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Section: Discussionmentioning

confidence: 99%

Cell-type-specific characterization of miRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution

de Sande,

Turunen,

Bouvy-Liivrand

et al. 2023

Preprint

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“…Studies have demonstrated that different miRNAs play a role in the regulation of age-related illnesses, including AD, age-related macular degeneration, neurological disorders, and cancer ( Natoli and Fernando, 2018 ; Eshkoor et al, 2022 ). Studies have shown that nutritional components and nutraceuticals can control the activity of miRNAs that have a role in age-related disorders.…”

Section: Implications For Prevention and Treatment Of Age-related Dis...mentioning

confidence: 99%

Decoding the secrets of longevity: unraveling nutraceutical and miRNA-Mediated aging pathways and therapeutic strategies

Salama,

Eissa,

Doghish

et al. 2024

Front. Aging

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MicroRNAs (miRNAs) are short RNA molecules that are not involved in coding for proteins. They have a significant function in regulating gene expression after the process of transcription. Their participation in several biological processes has rendered them appealing subjects for investigating age-related disorders. Increasing data indicates that miRNAs can be influenced by dietary variables, such as macronutrients, micronutrients, trace minerals, and nutraceuticals. This review examines the influence of dietary factors and nutraceuticals on the regulation of miRNA in relation to the process of aging. We examine the present comprehension of miRNA disruption in age-related illnesses and emphasize the possibility of dietary manipulation as a means of prevention or treatment. Consolidating animal and human research is essential to validate the significance of dietary miRNA control in living organisms, despite the abundance of information already provided by several studies. This review elucidates the complex interaction among miRNAs, nutrition, and aging, offering valuable insights into promising areas for further research and potential therapies for age-related disorders.

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“…Numerous factors influence miRNA expression levels, including age (Eshkoor et al, 2022; Jin Jung & Suh, 2012), gender (Lin, Han, et al, 2022; Lin, Liu, et al, 2022; Lin, Wu, et al, 2022), lifestyle (Panico et al, 2021), and an individual's disease history (Li & Kowdley, 2012). Multiple factors collaboratively influence the expression level of miRNA.…”

Section: Summary and Future Perspectivementioning

confidence: 99%

Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies

Zhang,

Liu,

Dong

et al. 2024

WIREs Nanomed Nanobiotechnol

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MicroRNA (miRNA), crucial non‐coding RNAs, have emerged as key biomarkers in molecular diagnostics, prognosis, and personalized medicine due to their significant role in gene expression regulation. Salivary miRNA, in particular, stands out for its non‐invasive collection method and ease of accessibility, offering promising avenues for the development of point‐of‐care diagnostics for a spectrum of diseases, including cancer, neurodegenerative disorders, and infectious diseases. Such development promises rapid and precise diagnosis, enabling timely treatment. Despite significant advancements in salivary miRNA‐based testing, challenges persist in the quantification, multiplexing, sensitivity, and specificity, particularly for miRNA at low concentrations in complex biological mixtures. This work delves into these challenges, focusing on the development and application of salivary miRNA tests for point‐of‐care use. We explore the biogenesis of salivary miRNA and analyze their quantitative expression and their disease relevance in cancer, infection, and neurodegenerative disorders. We also examined recent progress in miRNA extraction, amplification, and multiplexed detection methods. This study offers a comprehensive view of the development of salivary miRNA‐based point‐of‐care testing (POCT). Its successful advancement could revolutionize the early detection, monitoring, and management of various conditions, enhancing healthcare outcomes.This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > Diagnostic Nanodevices

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MicroRNAs influence and longevity

Cited by 7 publications

References 179 publications

Cell-type-specific characterization of miRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution

Cell-type-specific characterization of miRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution

Decoding the secrets of longevity: unraveling nutraceutical and miRNA-Mediated aging pathways and therapeutic strategies

Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies

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