MicroRNAs Signature in IL-2-Induced CD4+ T Cells and Their Potential Targets

Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs regulated gene expression at the post-transcriptional level. Many studies have investigated role of miRNAs in the biological processes such as proliferation, apoptosis, differentiation, and development. To evaluate role of miRNAs in proliferation and death of T cell, we performed miRNA profiling in activated CD4+ T cells after IL-2 induction and depletion. Proliferation rate of IL-2-induced cells was measured by MTT assay. Then quantitative RT-PCR arrays on 739 miRNAs revealed up- and down-regulation of 170 miRNAs in IL-2-induced CD4+ T cells relative to IL-2-depleted ones. In addition, in silico analysis predicted miRNA's potential targets in pathways such as JAK/STAT and PI3K pathways. JAK1 expression, a potential target of modulated miRNAs, was decreased in IL-2-depleted cells. This study suggests that clonal expansion is regulated by miRNAs in the absence or presence of IL-2 by targeting genes implicated in JAK/STAT and PI3K pathways.

The Expression of Nuclear Transcription Factor Kappa B (NF-κB) in the Case of Critically Ill Polytrauma Patients with Sepsis and Its Interactions with microRNAs

“…Moreover, due to this altered structure of the endothelium as well as due to hyper production of pro-inflammatory molecules, the capillary permeability is increased. Aggressive production of proinflammatory cytokines is because of the special biochemical interactions between the lipopolysaccharides-binding protein (LBP) and its receptor CD14, situated on the surface of macrophages (Hafizi et al 2012;Luan et al 2015;Ranji et al 2015;Bakula et al 2016;Boursier et al 2016). From the point of view of molecular expression of the pro-inflammatory cells, in the literature it is clearly mentioned that there is an excessive rise in few series of interleukins, like interleukin 1 (IL-1), interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 12 (IL-12), interleukin 17 (IL-17), interleukin 13 (IL-13) and interleukin 10 (IL-10) (Daniel Trancȃ et al 2014;Singer 2014;Dumache et al 2015).…”

Evaluation of the Expression of Circulating miR-16 and miR-26a in the plasma of Gastric Cancer Patients in Guilan province, North of Iran

“…Moreover, the study of the relationship between the expression of microRNAs and carcinogenesis of GC has been a challenge for researchers so far (27,28). Currently, miRNA-based methods are great candidates for the development of new generations of biomarkers and target therapies for immune diseases and cancers (29). In this study, the expression levels of miR-16 and miR-26a were investigated in 20 plasma samples of GC patients and compared to those in healthy individuals.…”