Microsatellite analysis of the parental contribution of Piaractus mesopotamicus to the production of offspring in the semi-natural system of reproduction

Povh, Jayme Aparecido; Ribeiro, Ricardo Pereira; Sirol, R.N.; Jr, Danilo Streit; Moreira, Heden Luiz Marques; Siewerdt, Frank; Lopera-Barrero, Nelson Maurício; Mangolin, Claudete Aparecida; Vargas, Lauro

doi:10.1590/s1516-89132010000200018

Cited by 11 publications

(13 citation statements)

References 19 publications

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“…This disequilibrium may be due to the number of samples collected. These results are similar to those obtained by Povh et al (2010) where they observed different patterns of linkage disequilibrium in the parental and progeny fishes, without affecting the estimates of genetic diversity.…”

Section: Resultssupporting

confidence: 90%

“…The breeding system used in mating (Lopera Povh et al, 2010) and the use of sequential or simultaneous semen during artificial fertilization (Withler and Beacham, 1994) are also factors that may affect the genetic variability of progeny used in stocking programs. Reproductive management is of fundamental importance to preserve the genetic diversity of juveniles which will participate in the stocking programs.…”

Section: Resultsmentioning

confidence: 99%

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Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil

Povh¹,

Ribeiro

Lopera-Barrero³

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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Monitoring the genetic diversity has fundamental importance for fish stocking programs. This experiment aims to evaluate the genetic diversity in two hatchery stations (A and B) with pacu Piaractus mesopotamicus (Holmberg, 1887) in Andirá, state of Paraná, Brazil used in stocking programs of Paranapanema River. Six microsatellite loci were amplified using DNA extracted from 60 fin-clipping samples. The broodstock B had the average number of alleles and the mean heterozygosity (alleles: 3.7 and H O : 0.628) higher than the broodstock A (alleles: 3.5 and H O : 0.600). Alleles with low frequency levels were observed in the both broodstocks. Evidence of linkage disequilibrium and lower allelic richness was found only in the broodstock A. Nei's gene diversity was high in both broodstocks. The genetic distance (0.085) and identity (0.918) showed similarity between broodstocks, which reflects a possible common origin. 6.05% of the total genetic variance was due to differences among broodstocks. Recent bottleneck effect in two broodstocks was observed. The results indicated a higher genetic diversity in the two broodstocks and they presented low genetic difference. This was caused by the reproductive management in both hatchery stations, reduction of population size and genetic exchange between the hatchery stations. Key words: Piaractus mesopotamicus, genetic variability, hatchery stations, molecular markers 6,05% da variância genética total foi devida a diferenças entre os estoques. Foi observado um recente efeito gargalo nos dois estoques. Os resultados indicaram uma alta diversidade genética nos estoques de reprodutores e baixa diferenciação genética entre eles, o que foi causado pelo manejo reprodutivo das pisciculturas, redução do tamanho populacional e intercâmbio genético entre as pisciculturas.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil

Povh¹,

Ribeiro

Lopera-Barrero³

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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“…These results were expected because the broodstocks were formed from the identical ancestral, from the Paraná River (foundation effect), and identical individuals were introduced from the Tietê and Grande rivers. Additional factors, such as the raising system (i.e., a small group of broodstocks, sexual proportions, reproductive dominance, endogamy, high variations among individuals during reproduction) and fluctuation in population size over time, can also be influenced by the homogeneity of current broodstocks (CAMERON-BROWN et al, 2005;BORREL et al, 2007;FOPP-BAYAT et al, 2010;LOPERA-BARRERO et al, 2010b;POVH et al, 2010).…”

Section: Genetic Structurementioning

confidence: 99%

“…Although these programs have been performed over several decades, studies of genetic and environmental efficiencies are necessary (AGOSTINHO et al, 2005;POVH et al, 2010). However, many programs have been unsuccessful because of the inappropriate management of broodstocks (BSs) (BORREL et al, 2007;LOPERA-BARRERO, 2009) and the non-monitoring of WPs.…”

Section: Introductionmentioning

confidence: 99%

“…Only a few studies regarding variability and genetic structure using microsatellites markers have been performed in P. mesopotamicus WPs and BSs (CALCAGNOTTO;DESALLE, 2009;LOPERA-BARRERO et al, 2010a;POVH et al, 2010POVH et al, , 2011. The microsatellite marker is the most used technique to achieve this purpose and has Semina: Ciências Agrárias, Londrina, v. 36, n. 6, p. 3807-3826, nov./dez.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of pacu for restocking programs in the Tietê and Grande rivers, Brazil

Ribeiro

Lopera-Barrero

Santos³

et al. 2015

Sem. Ci. Agr.

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Piaractus mesopotamicus is a tropical fish under threat because of declines in the number of wild populations. Restocking programs have been developed as a conservation method, and the genetic monitoring of populations and broodstocks is important to ensure the viability of such programs. The objective of the current unprecedented experiment was to evaluate the genetic diversity of P. mesopotamicus wild populations (WPs) and broodstocks (BSs) using microsatellites markers for supplying the restocking programs in the Tietê and Grande rivers. Six microsatellite loci were amplified using the DNA obtained from the caudal fin of 279 adult fish. The observed intrapopulation genetic variability was high with mean heterozygosity ranging from 0.203 to 0.833. The number of alleles per locus ranged from three (loci Pme28 and Pme32) to thirteen (loci Pme4, Pme5 and Pme14), and there were allele differences between WPsxWPs and WPsxBSs. This differentiation was confirmed by the dendrogram analysis that showed three specific clusters. Four alleles were shared in the WPs2012xBSs. Positive FIS values indicated the presence of endogamy in seven out of ten samples obtained from the WPs. The AMOVA analysis and FST values indicated moderate and high genetic differences in WPsxWPs and high genetic differences in WPsxBSs. The genetic distance and genetic identity values and number of migrants confirmed these results. Adequate intrapopulation genetic variability, similarity between BSsxBSs, and genetic differences between WPs2011xWPs2012 and WPsxBSs were observed. In the wild, individuals from the restocking program were partially observed. ResumoPiaractus mesopotamicus é um peixe tropical que nos últimos anos tem apresentado uma diminuição no número de populações naturais. Programas de repovoamento vêm sendo utilizados como método de conservação, entretanto, o monitoramento genético das populações e dos estoques de reprodutores é importante para conferir a viabilidade desse tipo de programas. O objetivo do presente estudo foi avaliar de forma inédita a diversidade genética de populações selvagens (WPs) e estoques de reprodutores (BSs) de P. mesopotamicus utilizados em programas de repovoamento dos rios Tietê e Grande, através de marcadores microssatélite. Seis loci microssatélite foram amplificados usando DNA extraído de nadadeira caudal de 279 indivíduos adultos. Foi observada alta variabilidade genética intrapopulacional, com medias de heterozigosidade observada entre 0.203 e 0.833. O número de alelos por locus foi de três (locus Pme28 e Pme32) a 13 (locus Pme4, Pme5 e Pme14) e houve diferenciação de alelos entre WPsxWPs e WPsxBSs. Essa diferenciação foi confirmada pela análise do dendrograma que mostrou a formação de três agrupamentos específicos. Observaram-se quatro alelos compartilhados entre WPs2012xBSs. Valores positivos de F IS mostraram a presença de endogamia em sete das 10 coletas realizadas nas WPs. A análise de AMOVA e do F ST indicou moderada e muito alta diferenciação genética entre WPsxWPs e diferenciação gen...

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Natural and artificial spawning strategies with fresh and cryopreserved semen in Rhamdia quelen: Reproductive parameters and genetic variability of offspring

Goes

Ribeiro

et al. 2017

Theriogenology

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Microsatellite analysis of the parental contribution of Piaractus mesopotamicus to the production of offspring in the semi-natural system of reproduction

Cited by 11 publications

References 19 publications

Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil

Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil

Genetic diversity of pacu for restocking programs in the Tietê and Grande rivers, Brazil

Natural and artificial spawning strategies with fresh and cryopreserved semen in Rhamdia quelen: Reproductive parameters and genetic variability of offspring

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