Microsatellite-Based Genetic Diversity Assessment in Grape (Vitis viniferaL) Germplasm and its Relationship with Agronomic Traits

Abstract

The population of grape (Vitis vinifera L), commonly cultivated in India, shows a wide range of ripening periods and fruit quality and is an unexploited resource for breeding programs. The main purpose of this study was to fingerprint these accessions and to construct a molecular database including the cultivars commonly grown in India. A total of 42 genotypes were analyzed using seven microsatellite simple sequence repeat (SSR) markers and their main morphological and agronomic characteristics were compared. A total of 45 alleles in all 42 genotypes were obtained with 7 primers with an average number of 6.4 alleles per locus. The dissimilarity matrix showed that a maximum of 110 units was obtained between the genotypes "Convent Large Black" and "Arka Hans," while a minimum dissimilarity if 37 units were obtained between the genotypes "Anab-e-Shahi" and "Dilkhush." In the dendrogram the microsatellite segregated the genotypes into two clusters (A and B) at 476 units with two subclusters each. The subclusters grouped genotypes predominantly as A1 with seeded fruits, A2 with seedless fruits, B1 with pigmented and seedless fruits, and B2 with colorless and seeded fruits. The use of seven polymorphicThe authors are grateful to the University of Agricultural Sciences, Bangalore and Indian Institute of Horticulture research, Bangalore for providing the facilities.Genetic Diversity in the Grape (Vitis vinifera L.) by Microsatellite Markers 93 microsatellite markers and the level of genetic variability detected within Indian grapevine germplasm suggested that this is a reliable, efficient, and effective marker system that can be used for diversity analysis and subsequently in crop improvement programs.

Microsatellite Markers and Genetic Diversity Analysis in Grape (Vitis vinifera L)

“…SUMMERY In summary, this study, using microsatellite markers on Indian grape vine genotypes, showed considerable genetic diversity existing among the population. This is most likely due to different conditions under which the populations are grown and conserved (Narayanaswamy et al, 2009). The clustering of the genotypes was predominantly based on the pigment and seed charac-ters in fruits.…”

Genotype identification and inference of genetic relatedness among different purpose grape varieties and rootstocks using microsatellite markers

“…Highly saturated linkage maps based on microsatellite markers alone (Adam Blondon et al, 2004;Doligez et al, 2006;Gaspero et al, 2007 ) or their integration with other type of markers (Vezzulli et al, 2008) are available in grapes. Microsatellite markers have proved to be useful in grapes for resolving the identity of related genotypes (Jahanke et al, 2007), fingerprinting (Regner et al, 2001;Riaz et al, 2008;Prins et al, 2009) and diversity analysis of cultivars (Martinez et al, 2006;Riaz et al, 2008;Schneider et al, 2009;Patricia et al, 2009;Papanna et al, 2009), rootstocks (Upadhyay et al, 2007) and cultivars, rootstocks and interspecific hybrids (Laucou et al, 2011).…”