Microsatellite DNA Polymorphism Analysis in a Case of an Illegal Cattle Purchase

Beamonte, Diego; Guerra, Amor; Ruiz, Begoña Otero; Alemany, J.

doi:10.1520/jfs13853j

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…Microsatellite loci are highly polymorphic regions composed of repeat units of between 2 and 5 base pairs (bp) that are arranged in tandem array and maintain allelic diversity based on differences in the array size (Bruford & Wayne, 1993). Ungulate microsatellite loci have been used in forensic identifications and paternity assignment in cattle (Beamonte et al . 1995).…”

Section: Introductionmentioning

confidence: 99%

Application of DNA markers to identify the individual‐specific hosts of tsetse feeding on cattle

Torr

Wilson

Schofield

et al. 2001

Medical Vet Entomology

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Primer sets for five different ungulate loci were used to obtain individual microsatellite DNA profiles for 29 Mashona cattle from a herd in Zimbabwe. There were 3-13 alleles for each locus and, using the entire suite of five loci, each animal within the herd, including closely related individuals, could be unequivocally distinguished. Wild-caught Glossina pallidipes Austen (Diptera: Glossinidae) were fed on specific cattle and the bloodmeal was profiled 0.5-72 h after feeding. The individual specific sources of the bloodmeals, including mixe meals produced by allowing tsetse to feed on two different cattle, were reliabl identified up to 24 h after feeding. The technique was used in field studies of hos selection by G. pallidipes and G. morsitans morsitans Westwood (Diptera Glossinidae) attracted to pairs of cattle. When the pair comprised an adult and a calf, 100% of meals were from the adult. For some pairs of adult cattle, tsetse were biased significantly towards feeding on one animal, whereas for other pairs there was no such bias. In general, feeding was greater on the animal known to have lower rate of host defensive behaviour. Results suggest that relatively slight differences in the inherent defensive behaviour of cattle produce large difference in host-specific feeding rates when the hosts are adjacent. For flies attracted to pair of cattle, < 2% contained blood from both hosts. The DNA profiling technique will be useful in studying the epidemiology of vector-borne diseases of livestock.

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Section: Introductionmentioning

confidence: 99%

Application of DNA markers to identify the individual‐specific hosts of tsetse feeding on cattle

Torr

Wilson

Schofield

et al. 2001

Medical Vet Entomology

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“…Parentage analysis has been used for some time to identify the origin of suspected stolen animals. In Spain, stolen calves were identified by comparing profiles with cows on the victim's farm (Beamonte et al 1995). Recently, our laboratory processed a case where a rancher in Texas filed suit claiming ownership of a calf nursing on his neighbor's cow.…”

Section: Recent Legal Cases Involving Dna Testing In Animal Forensicsmentioning

confidence: 99%

Dna Testing in Animal Forensics

Cassidy¹,

Gonzales²

2005

Journal of Wildlife Management

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The use of short tandem repeats (STRs) for the identification of animals has developed alongside similar STR applications for humans. Population studies, kinship analysis, paternity testing, and unique identification have been applied to humans and many other animals. The field of forensic science has adapted this information extensively in the prosecution of suspected criminals with great success. The power of human DNA testing is demonstrated routinely by the convictions and exonerations of individuals. We discuss how the use of nonhuman DNA testing is beginning to find a place in the prosecution of individuals. Animal evidence can be an important element in a case when used to establish an association between a crime scene and a suspect or in crimes involving a specific animal. Although the testing of animal evidence may be routine, its use in court is far less common. As the presentation of animal evidence in court increases, appropriate standards and guidelines must be applied to assure the admissibility of the DNA testing results in court. This requires rigorous validation of precision and accuracy, allele heritability and independence, accurate sampling, evidence handling, and appropriate statistical evaluation of the results before the criminal courts will accept and apply the power of DNA testing in cases involving animals.

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“…Genetic testing of domestic animal biological materials has been used to connect victims, perpetrators and/or crime scenes (Beamonte et al . ; Menotti‐Raymond et al . ; Schneider et al .…”

Section: Introductionmentioning

confidence: 99%

Review: domestic animal forensic genetics – biological evidence, genetic markers, analytical approaches and challenges

Kanthaswamy

2015

Animal Genetics

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This review highlights the importance of domestic animal genetic evidence sources, genetic testing, markers and analytical approaches as well as the challenges this field is facing in view of the de facto 'gold standard' human DNA identification. Because of the genetic similarity between humans and domestic animals, genetic analysis of domestic animal hair, saliva, urine, blood and other biological material has generated vital investigative leads that have been admitted into a variety of court proceedings, including criminal and civil litigation. Information on validated short tandem repeat, single nucleotide polymorphism and mitochondrial DNA markers and public access to genetic databases for forensic DNA analysis is becoming readily available. Although the fundamental aspects of animal forensic genetic testing may be reliable and acceptable, animal forensic testing still lacks the standardized testing protocols that human genetic profiling requires, probably because of the absence of monetary support from government agencies and the difficulty in promoting cooperation among competing laboratories. Moreover, there is a lack in consensus about how to best present the results and expert opinion to comply with court standards and bear judicial scrutiny. This has been the single most persistent challenge ever since the earliest use of domestic animal forensic genetic testing in a criminal case in the mid-1990s. Crime laboratory accreditation ensures that genetic test results have the courts' confidence. Because accreditation requires significant commitments of effort, time and resources, the vast majority of animal forensic genetic laboratories are not accredited nor are their analysts certified forensic examiners. The relevance of domestic animal forensic genetics in the criminal justice system is undeniable. However, further improvements are needed in a wide range of supporting resources, including standardized quality assurance and control protocols for sample handling, evidence testing, statistical analysis and reporting that meet the rules of scientific acceptance, reliability and human forensic identification standards.

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Microsatellite DNA Polymorphism Analysis in a Case of an Illegal Cattle Purchase

Cited by 6 publications

References 22 publications

Application of DNA markers to identify the individual‐specific hosts of tsetse feeding on cattle

Application of DNA markers to identify the individual‐specific hosts of tsetse feeding on cattle

Dna Testing in Animal Forensics

Review: domestic animal forensic genetics – biological evidence, genetic markers, analytical approaches and challenges

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