Microsatellite loci analysis for individual identification in Shiba Inu

Arata, Sayaka; Asahi, Ai; Takeuchi, Yukari; Mori, Yuji

doi:10.1292/jvms.15-0579

Cited by 4 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the advancement of science and technology, new genetic markers, such as single nucleotide polymorphisms, have become widely used; however, due to the cost and time of analysis, STR typing is still used in biodiversity and kinship analysis [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Microsatellite DNA Analysis of Genetic Diversity and Parentage Testing in the Popular Dog Breeds in Poland

Radko

Podbielska

2021

Genes

View full text Add to dashboard Cite

There is growing concern that extreme breed standardization contributes to a reduction of the effective population size and high levels of inbreeding, resulting in the loss of genetic diversity in many breeds. This study examined genetic diversity among eight popular dog breeds in Poland and evaluated the effectiveness of a 21-microsatellite (STR) panel recommended by the International Society for Animal Genetics (ISAG) for parent verification. The following breeds were characterized: German Shepherd, Maltese, Irish Wolfhound, Yorkshire Terrier, Biewer Yorkshire Terrier, Golden Retriever, Labrador Retriever, and French Bulldog. STRUCTURE analysis showed breed distinctiveness among all the dog breeds under study. Reynold’s distance ranged between θw = 0.634 and θw = 0.260. The studied breeds showed a medium level of genetic differentiation; the mean number of alleles per locus ranged from 3.4 to 6.6, and the effective number of alleles from 2.1 to 3.5. The mean degree of heterozygosity varied from 49% to 69% and from 47% to 68% for HO and HE, respectively. The population inbreeding coefficient (FIS) indicated an absence of inbreeding in the studied breeds. The average polymorphism information content (PIC) values for most of the breeds were higher than 0.5. The cumulative power of discrimination (PD) for all the markers in all breeds reached high values (close to 1.0), while the probability of identity (PID) was low, ranging between 10−11 and 10−19. The cumulative exclusion probability when the genotypes of one (PE1) and both parents (PE2) are known and showed that the parentage can be confirmed with a probability of 94.92% to 99.95% and 99.78% to 99.9999%, respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Microsatellite (STR) markers are a well-known effective and powerful tool widely used to investigate the genetic structure and diversity of dog breeds [ 9 , 10 , 11 , 12 , 13 ]. They are also the most important markers used for dog identification and parentage verification [ 9 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Microsatellite DNA Analysis of Genetic Diversity and Parentage Testing in the Popular Dog Breeds in Poland

Radko

Podbielska

2021

Genes

View full text Add to dashboard Cite

show abstract

“…Over the past several decades, MS has been an effective tool in studies to determine bloodlines and evaluate genetic structure, and genetic diversity of dog breeds/populations (Bigi et al, 2015;Arata et al, 2016;Radko et al, 2018;Goleman et al, 2019). Selection and cross-breeding can lead to similarities in certain traits in individuals.…”

Section: Genetic Similarity and Phylogenymentioning

confidence: 99%

Analysis of genetic diversity of Ven dog breed based on microsatellite markers

Nguyen Thi,

Le,

Huynh Thi

et al. 2024

AJB

View full text Add to dashboard Cite

Microsatellite (MS) is a genetic marker widely used in the studies of pedigree, individual identification, gene mapping, and genetic diversity within and between populations. The genetic diversity of the indigenous Ven dog breed, along with two imported dog breeds, the Berger and Poodle dogs raised in Vietnam, was analyzed based on seven microsatellite markers. A total of 32 alleles, an average number of alleles/loci of 4.6 were observed across 80 samples of the three dog breeds. The overall polymorphic information content (PIC) was 0.67 representing the quality of selected MS markers. Genetic diversity indices (Ho, He, Fit, Fst) showed the phenomenon of inbreeding between individuals in the Ven dog population. The genetic similarity level and phylogenetic tree also reasonably reflect the genetic relationship between the three analyzed dog breeds, in which the Ven dog showed a higher genetic distance compared to two imported dog breeds.

show abstract