Microsatellite markers for the prized matsutake mushroom (Tricholoma matsutake, Tricholomataceae)

Abstract

Premise of the StudyNovel and cost‐effective microsatellite markers were developed to explore the population genetics, biogeographic structure, and evolutionary history of the prized Euro‐Asian wild edible ectomycorrhizal fungus Tricholoma matsutake (Tricholomataceae).Methods and resultsEighteen new polymorphic simple sequence repeat loci, detected from a microsatellite‐enriched genomic library, were used to characterize 131 individuals from eight T. matsutake populations. The number of alleles ranged from two to 10, with averages of 1.42 to 3.22. Levels of observed and expected heterozygosity ranged from 0.00–1.00 and from 0.00–0.83, with mean values of 0.21 and 0.26, respectively. In total, 50% of the loci showed interspecific transferability and polymorphism in the related species T. equestre.ConclusionsThese newly developed markers will aid research into the genetic diversity and population structure of T. matsutake. They can also be used in other species of Tricholoma.

Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Agaricus bisporus)

“…Previous studies obtained a PIC value of 0.612 for shiitake mushrooms [22], and PIC and H E values of 0.511 [21] and 0.67 [23], respectively, for pine mushrooms, indicating that the markers developed for other edible mushrooms showed a similar trend to that observed in the present study. Assessing the diversity of germplasm using DNA markers is very important and essential for selecting breeding materials.…”

“…To date, SSR markers have been developed from various biological resources including plants, such as rice and peas, and fungi [11,[18][19][20]. SSR markers have previously been developed for the mushrooms L. edodes [21,22], Tricholoma matsutake [23,24], and A. bisporus [25][26][27][28]. However, the majority of existing SSR markers are based on enriched sequencing [26,27], and further development of markers based on the entire A. bisporus genome is necessary.…”