Microsatellite markers for the red band needle blight pathogen, <i>Dothistroma septosporum</i>

Barnes, Irene; Cortinas, Maria-Noel; Wingfield, Brenda D.

doi:10.1111/j.1755-0998.2008.02142.x

Cited by 34 publications

(52 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They were also used for species identification in the whole‐conidiomata DNA samples. Mating types were determined for the D. pini isolates from Dieszel and Russia in this study and the D. pini isolates from Ukraine and Russia in the study by B arnes et al. (2008b).…”

Section: Methodsmentioning

confidence: 91%

“…(2007). Another approach that is useful for screening large populations of isolates is the microsatellite primer pair, Doth_A (B arnes et al. 2008b).…”

Section: Introductionmentioning

confidence: 99%

“…2008b). This primer can be used as a diagnostic marker in genescan analyses as the locus amplified is monomorphic for allele 124 in D. septosporum and 114 in D. pini (B arnes et al. 2008b).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, DNB has been regularly reported from new areas and having an increased intensity in pine plantations of Hungary. In a preliminary screening of isolates collected from infected P. nigra trees in this country, the microsatellite diagnostic marker, Doth_A, revealed the presence of a different allele of 109 bp, possibly that of D. pini , in addition to the 124‐bp allele of D. septosporum (B arnes et al. 2008b; B arnes 2009).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Needle blight of pine caused by two species of Dothistroma in Hungary

2010

View full text Add to dashboard Cite

Dothistroma septosporum and D. pini cause the serious pine needle disease, Dothistroma needle blight (DNB). Of these, D. septosporum has a global distribution, while D. pini is known only from the USA, Russia, Ukraine and France. During a study of D. septosporum isolates from Pinus nigra in Hungary, microsatellite markers revealed the presence of a second Dothistroma species. The aim of this study was to identify the DNB pathogens occurring in Hungary using four different molecular techniques. These included sequencing of the rDNA ITS region, a species-specific ITS-RFLP, mating type primers and a diagnostic microsatellite marker, Doth_A. Results showed that both D. septosporum and D. pini occur in Hungary and that both DNB pathogens were present on the same trees and in some cases, the same needles. Mating types MAT1 and MAT2 of D. septosporum were shown to be present in Hungary, but only the MAT2 of D. pini was found. In addition, examination of needles with DNB symptoms from Russia revealed the presence of D. pini on Pinus pallasiana and P. nigra as well as on a new host, Pinus mugo. The molecular markers applied in this study were sufficiently robust to identify and differentiate between the two DNB pathogens, both in culture and directly from needles. They will consequently be useful to document the geographical range and to monitor the spread of D. septosporum and D. pini in future studies.

show abstract

Section: Methodsmentioning

confidence: 91%

“…(2007). Another approach that is useful for screening large populations of isolates is the microsatellite primer pair, Doth_A (B arnes et al. 2008b).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Needle blight of pine caused by two species of Dothistroma in Hungary

2010

View full text Add to dashboard Cite

show abstract

“…The microsatellite markers Doth_DS1, Doth_DS2, Doth_E, Doth_F, Doth_G, Doth_I, Doth_J, Doth_K, Doth_L, Doth_M and Doth_O, developed by Barnes et al . (), were amplified and scored following the methods of Mullett et al . ().…”

Section: Methodsmentioning

confidence: 99%

Temporal changes in the population structure ofDothistroma septosporumat the site of the first recorded outbreak in Poland

et al. 2018

View full text Add to dashboard Cite

In this study, evolution of the genetic structure of the oldest known population of Dothistroma septosporum in Poland was analysed. Dothistroma needle blight was first recorded in Poland in 1990 in a 5‐year‐old plantation, meaning the population is relatively young in terms of evolution and genetic development. The preservation of DNA extracts from other studies allowed examination of the genetic variation of the pathogen over the span of a decade with the use of highly sensitive microsatellite markers. A number of evolutionary factors were identified that have shaped the genetic structure of this population over time. First, indications of the founder effect and the impact of selection were observed. Secondly, structure analysis provided evidence of the introduction of a new genetic component to the population structure after the pathogen had established. Finally, the impact of the reproductive mode on various components of the Domiarki population was examined, revealing the presence of genetic groups and structure clusters with varying levels of sexual recombination. These results demonstrate how well established and genetically robust a new population of a pathogen may become in just a few decades, given favourable conditions, and how new introductions may facilitate this process.

show abstract

Population structure and diversity of an invasive pine needle pathogen reflects anthropogenic activity

Barnes

Carbone

Kirisits

et al. 2014

Ecology and Evolution

113

View full text Add to dashboard Cite

Dothistroma septosporum is a haploid fungal pathogen that causes a serious needle blight disease of pines, particularly as an invasive alien species on Pinus radiata in the Southern Hemisphere. During the course of the last two decades, the pathogen has also incited unexpected epidemics on native and non-native pine hosts in the Northern Hemisphere. Although the biology and ecology of the pathogen has been well documented, there is a distinct lack of knowledge regarding its movement or genetic diversity in many of the countries where it is found. In this study we determined the global population diversity and structure of 458 isolates of D. septosporum from 14 countries on six continents using microsatellite markers. Populations of the pathogen in the Northern Hemisphere, where pines are native, displayed high genetic diversities and included both mating types. Most of the populations from Europe showed evidence for random mating, little population differentiation and gene flow between countries. Populations in North America (USA) and Asia (Bhutan) were genetically distinct but migration between these continents and Europe was evident. In the Southern Hemisphere, the population structure and diversity of D. septosporum reflected the anthropogenic history of the introduction and establishment of plantation forestry, particularly with Pinus radiata. Three introductory lineages in the Southern Hemisphere were observed. Countries in Africa, that have had the longest history of pine introductions, displayed the greatest diversity in the pathogen population, indicating multiple introductions. More recent introductions have occurred separately in South America and Australasia where the pathogen population is currently reproducing clonally due to the presence of only one mating type.

show abstract

Microsatellite markers for the red band needle blight pathogen, Dothistroma septosporum

Cited by 34 publications

References 10 publications

Needle blight of pine caused by two species of Dothistroma in Hungary

Needle blight of pine caused by two species of Dothistroma in Hungary

Temporal changes in the population structure ofDothistroma septosporumat the site of the first recorded outbreak in Poland

Population structure and diversity of an invasive pine needle pathogen reflects anthropogenic activity

Contact Info

Product

Resources

About