Microsatellite markers reveal a strong geographical structure in European populations of <i>Castanea sativa</i> (Fagaceae): Evidence for multiple glacial refugia

Mattioni, Claudia; Martín, L. M.; Pollegioni, Paola; Cherubini, M.; Villani, F.

doi:10.3732/ajb.1200194

Cited by 82 publications

(93 citation statements)

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“…The loci employed in this study identified a total of 59 alleles (Table 1), with an overall richness comparable to that reported in studies covering much larger areas (Gobbin et al 2007;Wang et al 2008;Martin et al 2009;Mattioni et al 2013;Lusini et al 2014). Our marker set was highly informative for the material studied and a large proportion of alleles (61 %) was detected at a frequency below 5 %.…”

Section: Genetic Diversity In El Bierzo Orchardssupporting

confidence: 77%

“…Recent studies using molecular markers have confirmed that the genetic landscape of C. sativa is complex across its distribution area-from the Atlantic Ocean to the Caspian Sea-especially in traditional nut production areas, where both grafted material and wild populations have coexisted for long periods of time (Gobbin et al 2007;Lang et al 2007;Mattioni et al 2008Mattioni et al , 2013Martin et al 2010). The northwest region of the Iberian Peninsula is particularly interesting in this context because (i) it is located at one extreme of the distribution area and concentrates the most extensive sweet chestnut stands in Spain and Portugal, (ii) it has been one of the major focal points for the native species after the last glaciation, and (iii) it has a centenary tradition of chestnut cultivation and management Ramos-Cabrer and PereiraLorenzo 2005;Pereira-Lorenzo et al 2010aMartin et al 2012;Mattioni et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The northwest region of the Iberian Peninsula is particularly interesting in this context because (i) it is located at one extreme of the distribution area and concentrates the most extensive sweet chestnut stands in Spain and Portugal, (ii) it has been one of the major focal points for the native species after the last glaciation, and (iii) it has a centenary tradition of chestnut cultivation and management Ramos-Cabrer and PereiraLorenzo 2005;Pereira-Lorenzo et al 2010aMartin et al 2012;Mattioni et al 2013). This region hosts nowadays prime orchards for commercial nut production, located mainly in Galicia and El Bierzo (Castile and Leon) (Rubio 2009).…”

Section: Introductionmentioning

confidence: 99%

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Genetic characterization of chestnut (Castanea sativa Mill.) orchards and traditional nut varieties in El Bierzo, a glacial refuge and major cultivation site in northwestern Spain

Quintana

Contreras

Merino

et al. 2014

Tree Genetics & Genomes

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This paper presents a detailed genetic study of Castanea sativa in El Bierzo, a major nut production region with interesting features. It is located within a glacial refuge at one extreme of the distribution area (northwest Spain); it has a centenary tradition of chestnut management; and more importantly, it shows an unusual degree of genetic isolation. Seven nuclear microsatellite markers were selected to analyze the genetic variability and structure of 169 local trees grafted for nut production. We analyzed in the same manner 62 local nuts. The selected loci were highly discriminant for the genotypes studied, giving a combined probability of identity of 6.1 x 10~6. An unprecedented density of trees was sampled for this project over the entire region, and nuts were collected representing 18 cultivars marketed by local producers. Several instances of misclassification by local growers were detected. Fixation index estimates and analysis of molecular variance (AMOVA) data are supportive of an unexpectedly high level of genetic differentiation in El Bierzo, larger than that estimated in a previous study with broader geographical scope but based on limited local sampling (Pereira-Lorenzo et al., Tree Genet Genomes 6: 701-715, 2010a). Likewise, we have determined that clonality due to grafting had been previously overestimated. In line with these observations, no significant spatial structure was found using both a modelbased Bayesian procedure and Mantel's tests. Taken together, our results evidence the need for more fine-scale genetic studies if conservation strategies are to be efficiently improved.

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Section: Genetic Diversity In El Bierzo Orchardssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic characterization of chestnut (Castanea sativa Mill.) orchards and traditional nut varieties in El Bierzo, a glacial refuge and major cultivation site in northwestern Spain

Quintana

Contreras

Merino

et al. 2014

Tree Genetics & Genomes

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“…These markers have proved a useful tool for determining the genetic structure in many plant species (Mattioni et al 2013, Lavor et al 2014, Noormohammadi et al 2014, Shivaprakash et al 2014. In wild Phaseolus lunatus, SSRs have been used to determine the genetic structure in populations of Costa Rica and Mexico, showing high genetic differentiation between wild populations of P. lunatus and clustering patterns based on geographical isolation and low levels of gene flow (Ouédraogo et al 2005, Martínez-Castillo et al 2006.…”

Section: K Pedro Jesús Ruiz-gil Et Almentioning

confidence: 99%

Differentiation and genetic diversity of Phaseolus lunatus wild populations from Chiapas, Mexico, and their genetic relationships with MI and MII groups

et al. 2016

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Background: Understanding the genetic structure of wild relatives of domesticated species is crucial for its conservation and to elucidate the sites of crop domestication. Lima bean is one of the five domesticated Phaseolus species and Mexico is one of its centers of domestication. Recent studies showed the existence of two wild gene pools (MI and MII) of this species in Mexico and suggested that their genetic divergence occurred in southeast Mexico. Question: Did MI and MII groups diverged around the Isthmus of Tehuantepec, Mexico? Studied species: Lima bean: Neotropical plant species, herbaceous, with an annual/short life cycle and with autogamous tendency. Study site and years of study: Seven wild populations were collected in 2012 in the State of Chiapas, México, area underrepresented in previous studies. Methods: Genetic diversity and grouping patterns of collected populations and their relationship to MI and MII groups were analyzed at eight microsatellite loci. Results: High genetic structure (F ST : 0.42 to 0.96) and a high level of genetic diversity (H E = 0.48) were found. The analyses, and presence of admixed populations in MI and MII, suggested that the genetic divergence of these groups is an ongoing process centered around the Isthmus of Tehuantepec. Conclusions: Our results support the hypothesis that MI and MII groups diverged around the Isthmus of Tehuantepec; however, sampling should be increased both at population and genomic levels, to determine the precise organization of the genetic diversity of wild P. lunatus from Mexico. Keywords: Genetic diversity, Genetic resources, Lima bean, Microsatelites, SSR markers, Wild relatives. Diversidad y diferenciación genética de las poblaciones silvestres de Phaseolus lunatus de Chiapas, México, y sus relaciones genéticas con los grupos MI y MII ResumenAntecedentes: Entender la estructura genética de los parientes silvestres de las especies domesticadas es crucial para su conservación y para elucidar los sitios de domesticación de los cultivos. El frijol Lima es una de las cinco especies domesticadas del género Phaseolus y México es uno de sus centros de domesticación. Estudios recientes señalaron dos acervos genéticos silvestres (MI y MII) en México y sugirieron que su divergencia ocurrió en el sureste de México. Pregunta: ¿Los grupos silvestres MI y MII divergieron alrededor del Istmo de Tehuantepec, México? Especie en estudio: El frijol Lima es una especie Neotropical, herbácea, con ciclo de vida anual/corto y tendencia autógama. Sitio y años de estudio: Siete poblaciones silvestres fueron colectadas en 2012 en el Estado de Chiapas, México, área que fue subrepresentada en estudios previos. Métodos: Se analizó la diversidad genética y los patrones de agrupamiento de las poblaciones colectadas y su relación con los grupos MI and MII, usando ocho loci de microsatélites. Resultados: Se encontró una estructura genética alta (F ST : 0.42 a 0.96) y niveles altos de diversidad gené-tica (H E = 0.48). Los análisis y la presencia de poblaciones mezclada...

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“…can be found over a wide range of climatic conditions in Europe, and its current distribution is the result of natural colonization and human intervention (Conedera et al, 2004;Mattioni et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Short communication: Functional genetic diversity of chestnut (Castanea sativa Mill.) populations from southern Spain

et al. 2018

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Aim of the study: To evaluate the adaptive genetic variability of chestnut (Castanea sativa Mill.) populations from southern Spain in relation to bud burst and water stress.Area of study: Andalusia (southern Spain) where many chestnut groves were progressively abandoned and have become 'naturalized'. Material and methods:A total of 126 chestnut trees from eight populations were assessed by means of nine genic microsatellite loci (expressed sequence tag simple sequence repeat markers) related to bud burst and water stress.Main results: Significant differences in genetic diversity were detected within and among populations, not found with neutral microsatellite markers. The structure analysis indicated the presence of two different gene pools.Research highlights: These results could contribute to the development of conservation strategies for this species in southern areas exposed to the effects of climate change. The genetic diversity of these populations could be useful in minimizing this risk and other predictable factors related to global change.Additional keywords: functional markers; adaptation; population genetic structure. Abbreviations used: EST s (expressed sequence tag); SSR s (simple sequence repeats).Authors´ contributions: MIC conducted the laboratory analyses and wrote the paper. CM participated in the design of the experiment, data analysis, discussion and redaction of the paper. LMM conducted the collection of data in the field and coordinated the research project. EVO participated in the collection of data in the field. MC led the laboratory analysis. MAM supervised the work and participated in the design of the experiment, discussion and redaction of the paper. (Tables S1, S2) accompanies the paper on FS's website.

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Microsatellite markers reveal a strong geographical structure in European populations of Castanea sativa (Fagaceae): Evidence for multiple glacial refugia

Cited by 82 publications

References 54 publications

Genetic characterization of chestnut (Castanea sativa Mill.) orchards and traditional nut varieties in El Bierzo, a glacial refuge and major cultivation site in northwestern Spain

Genetic characterization of chestnut (Castanea sativa Mill.) orchards and traditional nut varieties in El Bierzo, a glacial refuge and major cultivation site in northwestern Spain

Differentiation and genetic diversity of Phaseolus lunatus wild populations from Chiapas, Mexico, and their genetic relationships with MI and MII groups

Short communication: Functional genetic diversity of chestnut (Castanea sativa Mill.) populations from southern Spain

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