Microsatellite markers: what they mean and why they are so useful

Vieira, Maria Lúcia Carneiro; Santini, Luciane; Diniz, Augusto Lima; Munhoz, Carla de Freitas

doi:10.1590/1678-4685-gmb-2016-0027

Cited by 642 publications

(488 citation statements)

References 166 publications

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“…Tandem repeats are evolutionarily relevant due to their instability since they mutate at rates between 10 3 and 10 6 per cell generation, i.e., up to 10 orders of magnitude greater than point mutations [22]. Microsatellites are used in genetic linkage analyses to locate genes or mutations responsible for a wide variety of diseases.…”

Section: Microsatellites and Single Nucleotide Polymorphisms (Snps)mentioning

confidence: 99%

Genetics and epigenetics in the pathogenesis of primary biliary cholangitis

Joshita

Umemura

Tanaka

et al. 2017

Clin J Gastroenterol

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Primary biliary cholangitis (PBC) is a chronic, slowly progressive cholestatic autoimmune liver disease predominantly afflicting women. PBC is characterized by the presence of disease-specific antimitochondrial antibodies and the histological destruction of intrahepatic bile ducts, which eventually lead to cirrhosis and hepatic failure. Fortunately, ursodeoxycholic acid therapy has improved the outcome of the vast majority of PBC cases. Although the etiology of PBC has not yet been elucidated, human leukocyte antigen (HLA) class II alleles have been consistently associated with disease onset for decades. PBC patients may also have genetically determined risk factors in non-HLA regions. Meanwhile, exposure to environmental factors, such as infectious diseases and harmful chemicals, can produce epigenetic alterations in some individuals and subsequent PBC onset. In this review, we describe the influence of HLA alleles and other gene polymorphisms on PBC along with the results of genome-wide association studies on this disease and its future prospects in terms of epigenetics.

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Section: Microsatellites and Single Nucleotide Polymorphisms (Snps)mentioning

confidence: 99%

Genetics and epigenetics in the pathogenesis of primary biliary cholangitis

Joshita

Umemura

Tanaka

et al. 2017

Clin J Gastroenterol

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“…Molecular marker technology, however, has improved breeders' ability to accurately identify and characterize genetic resources. DNA markers use nucleotide sequences to identify species (Ganie et al, 2015) and microsatellites or single sequence repeats (SSRs) are extensively employed in plant genetics studies, using both low and high throughput genotyping approaches (Vieira et al, 2016). Polymorphic DNA was chosen to characterize rice germplasm because it satisfies these criteria.…”

Section: Author(s) Agree That This Article Remain Permanently Open Acmentioning

confidence: 99%

Genetic diversity of rice from Iran region assessed by simple sequence repeat (SSR) markers

Vazirzanjani¹,

Kawai²,

Korrani³

et al. 2017

Afr. J. Agric. Res.

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Simple sequence repeat (SSR) markers (36 microsatellite pairs) were used to assess genetic diversity among sixteen rice (Oriza sativa L.) cultivars from Iran, four from Uzbekistan, and one from Afghanistan, and to compare this diversity with that of three control cultivars including Nipponbare, Jasmine 85 and Basmati 370. Among the 36 microsatellite pairs, 31 produced polymorphisms ranging from 1 to 7 alleles (average = 3.72 per microsatellite). There were 134 alleles detected using all the SSR primers, 103 of which (76.9%) showed polymorphisms, while 31 did not. The genetic similarity coefficient among the 24 rice cultivars was 0.601. The genetic diversity revealed by this survey will be useful to designate the most appropriate parental cv. to initiate a breeding program aimed at developing new rice varieties with traits adapted for Middle Eastern agriculture.

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“…They are widely present in eukaryotic genomes and very useful, mainly because of their codominant inheritance, high polymorphism, high variability and suitability for automated allele sizing and cross-species transferability (Kalia et al 2011, Vieira et al 2016)using both low and high throughput genotyping approaches. Motivated by the importance of these sequences over the last decades this review aims to address some theoretical aspects of SSRs, including definition, characterization and biological function.…”

Section: Introductionmentioning

confidence: 99%