Microsatellite variability in apricots (Prunus armeniaca L.) reflects their geographic origin and breeding history

Maghuly, Fatemeh; Fernández, E.; Ruthner, Szabolcs; Pedryc, A.; Laimer, Margit

doi:10.1007/s11295-005-0018-9

Cited by 73 publications

(55 citation statements)

References 35 publications

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“…This result is in agreement with the study of Maghuly et al (2005) who reported that Cacansko zlato, Maďarská C-235, Velkopavlovická (Le 1) and Luizet 343 cultivars were localized in one subgroup. The same result was confirmed by Maghuly et al (2006).…”

Section: Genetic Relationships and Clustering Of Cultivars By Means Osupporting

confidence: 93%

“…2, with Harogem, Leskora and Riland cultivars. Maghuly et al (2005) formed a group of cultivars of varied origin, among which were Orange Red, Harcot, Shalah, Churmai and Veecot.…”

Section: Genetic Relationships and Clustering Of Cultivars By Means Omentioning

confidence: 99%

“…2, part 4.2). Maghuly et al (2005) grouped cv. Priusadebnyj with Samarkandskij Rannij, using 10 polymorphic microsatellite markers.…”

Section: Genetic Relationships and Clustering Of Cultivars By Means Omentioning

confidence: 99%

“…2, part 4.4). Maghuly et al (2005) suggested that Kec Psar showed a very high level of genetic distance in the dendrogram. In addition Romero et al (2003) put the Kec Psar, Vnuk-partizana and Olymp cultivars in the same group.…”

Section: Genetic Relationships and Clustering Of Cultivars By Means Omentioning

confidence: 99%

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An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers

Chroboková¹,

Raddová²,

Vachůn³

et al. 2011

Hort. Sci. (Prague)

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Chroboková E., Raddová J., Vachůn M., Krška B., Pidra M., 2011. An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers. Hort. Sci. (Prague), 38: 125-133.The random amplified polymorphic DNA (RAPD) technique and microsatellites were used to study the genetic diversity and to identify cultivars within a collection of 95 cultivars of Prunus armeniaca L. A dendrogram based on 13 RAPD primers and a dendrogram based on 9 microsatellite primers were prepared using the unweighted pair group method with average (UPGMA) group analysis. In both dendrograms, the cultivars were classified into five groups, according to their geographic origin: hybrids originated by hybridization among cultivars of European and Asian origin, European cultivars, American cultivars, Asian cultivars and interspecific hybrids. Eleven cultivars were not distinguished (9 cultivars with supposed relatedness to Velkopavlovická cv., 2 cvs Vynoslivyj and Vynoslivyj 21/1 that are assumed to be clones) using 9 microsatellite primers. The similarities and the differences revealed among incorporation of cultivars into groups were compared with the literature findings. The results of these analyses have a direct implication on the selection of new breeding progenitors at the Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic.Keywords: Prunus armeniaca L.; microsatellites; RAPD primers; cultivar identification Fast and cost-effective identification of important plant cultivars in agriculture and horticulture as well as for practical breeding purposes and plant proprietary rights protection is very important. The random amplified polymorphic DNA (RAPD) molecular methods and microsatellites based on the DNA study are effective tools often used for these purposes.The RAPD method was reported by Williams et al. (1990). The greatest advantage of the RAPD approach is its technical simplicity, paired with the independence of any prior DNA sequence information. Molecular RAPD markers facilitate the study of genetic variability among Prunus rootstocks (Casas et al. 1999). This polymorphism was evaluated using 7 RAPD primers. Twenty-five apricot cultivars were analysed and 19 were distinguished using the RAPD markers (Mariniello et al. 2002). These markers were also successfully used for identifying and studying of the genetic relationships of grapes Moravcová et al. 2006;Pidra et al. 2006).Another method used for cultivar identification is the simple sequence repeat (SSR) approach. The existence of repeated simple sequence motives (also called microsatellites) in plant nuclear DNA was demonstrated by Delseny et al. (1983) and Tautz, Renz (1984). Microsatellites are ideal DNA markers for genetic mapping and population-genetic studies because of their abundance, co-dominant character, ease of detection by PCR, extensive genome coverage and requirement of a small amount of starting DNA.The first application of microsatellites in plants was in cultivar identification, where microsatellites were use...

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Section: Genetic Relationships and Clustering Of Cultivars By Means Osupporting

confidence: 93%

“…2, with Harogem, Leskora and Riland cultivars. Maghuly et al (2005) formed a group of cultivars of varied origin, among which were Orange Red, Harcot, Shalah, Churmai and Veecot.…”

Section: Genetic Relationships and Clustering Of Cultivars By Means Omentioning

confidence: 99%

“…2, part 4.2). Maghuly et al (2005) grouped cv. Priusadebnyj with Samarkandskij Rannij, using 10 polymorphic microsatellite markers.…”

Section: Genetic Relationships and Clustering Of Cultivars By Means Omentioning

confidence: 99%

Section: Genetic Relationships and Clustering Of Cultivars By Means Omentioning

confidence: 99%

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An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers

Chroboková¹,

Raddová²,

Vachůn³

et al. 2011

Hort. Sci. (Prague)

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show abstract

“…Previously, SSR markers have been widely used for molecular characterizations and similarity relationships among Prunus accessions and have revealed high polymorphism level to discriminate these accessions (Sosinski et al, 2000;Dirlewanger et al, 2002;Maghuly et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Simple sequence repeat-based assessment of genetic relationships among Prunus rootstocks

Turkoglu

Bilgener²,

Erċışlı³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Ten SSR loci, previously developed for Prunus, were analyzed to examine genetic relationships among 23 rootstock candidates for sweet and sour cherries, of the species P. avium, P. cerasus, P. mahaleb, and P. angustifolia. Five genotypes of P. laurocerasus, not used as rootstock, were included in the molecular analysis. The number of alleles per locus ranged from 8 to 12, with a mean of 9, while the number of microsatellite genotypes varied from 8 to 17, indicating that the SSRs were highly informative. The degree of heterozygosity (0.61) was high. Clustering analysis resulted in two main clusters. The first cluster was divided into two subclusters; the first subcluster consisted of P. avium and P. cerasus, and the second subcluster consisted of P. laurocerasus. The second cluster was divided into two subclusters. The first subcluster consisted of P. mahaleb genotypes and the second consisted of P. angustifolia genotypes. The reference rootstocks also clustered with their associated botanical species. Unweighted pair-group method with arithmetic mean analysis demonstrated that P. laurocerasus 2157 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (4): 2156-2165 (2010) SSR analysis in Prunus rootstocks genotypes had less genetic variation and that P. avium genotypes were more closely related to P. cerasus. The SSR-based phylogeny was generally consistent with Prunus taxonomy information, suggesting the applicability of SSR analysis for genotyping and phylogenetic studies in the genus Prunus.

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Virus Resistance Breeding in Fruit Trees

Laimer¹

2006

Tree Transgenesis

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Microsatellite variability in apricots (Prunus armeniaca L.) reflects their geographic origin and breeding history

Cited by 73 publications

References 35 publications

An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers

An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers

Simple sequence repeat-based assessment of genetic relationships among Prunus rootstocks

Virus Resistance Breeding in Fruit Trees

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