Microscale Flow Dynamics of Red Blood Cells in Microchannels: An Experimental and Numerical Analysis

Abstract

The book collects the state-of-the-art research, methods and new trends on the subject of computational vision and medical image processing contributing to the development of these knowledge areas. PrefaceNowadays, computational methodologies of signal processing and imaging analysis for 2D, 3D and even 4D data are commonly used for various applications in society. For example, Computational Vision systems are progressively used for surveillance tasks, traffic analysis, recognition process, inspection purposes, human-machine interfaces, 3D vision and deformation analysis.One of the main characteristics of the Computational Vision domain is its intermultidisciplinary nature. In fact, in this domain, methodologies of several other fundamental sciences, such as Informatics, Mathematics, Statistics, Psychology, Mechanics and Physics are regularly used. Besides this inter-multidisciplinary characteristic, one of the main rationale that promotes the continuous effort being made in this area of human knowledge is the number of applications in the medical area. For instance, statistical or physical procedures on medical images can be used in order to model the represented structures. This modelling can have different goals, for example: shape reconstruction, segmentation, registration, behavioural interpretation and simulation, motion and deformation analysis, virtual reality, computer-assisted therapy or tissue characterization.The main objective of the ECCOMAS Thematic Conferences on Computational Vision and Medical Image Processing (VIPimage) is to promote a comprehensive forum for discussion on the recent advances in the related fields and try to identify areas of potential collaboration between researchers of different sciences.This book contains the extended versions of nineteen papers selected from works presented at the second ECCOMAS thematic conference on Computational Vision and Medical Image processing (VIPimage 2009), which was held at the Engineering Faculty of the University of Porto, Portugal. It gathers together the state-of-the-art on the subject of Computational Vision and Medical Image processing contributing to the development of these knowledge areas and showing new trends in these fields.The Editors would like to take this opportunity to thank to the European Community on Computational Methods in Applied Sciences, the Abstract The blood flow dynamics in microcirculation depends strongly on the microvascular networks composed with short irregular vessel segments which are linked by numerous bifurcations. This paper presents the application of a confocal micro-PTV system to track RBCs through a rectangular polydimethysiloxane (PDMS) microchannel with a bifurcation. By using a confocal micro-PTV system, we have measured the effect of bifurcation on the flow behaviour of both fluorescent particles diluted in pure water and RBCs in concentrated suspensions. After performing simulations with the commercial finite element software package POLYFLOW R , some experimental results were compared with the nume...

In Vitro Blood Flow Behaviour in Microchannels with Simple and Complex Geometries

“…In contrast, for the case of labelled RBCs the trajectories are more asymmetric when compared with PW trajectories. Additionally, we can also observe several fluctuations on their trajectories possibly due to cell interactions enhanced by the high local Hct originated at this region (Lima et al, 2011a(Lima et al, , 2011b (Leble et al, 2011a(Leble et al, , 2011bLima et al, 2011).…”

“…18. Dimensions of the a) diverging and b) confluence used in this study (Leble et al, 2011a(Leble et al, , 2011bLima et al, 2011).…”

Microbubble moving in blood flow in microchannels: effect on the cell-free layer and cell local concentration

“…The influence of the CFL thickness by physical and rheological factors such as hematocrit, RBC deformability, vessel diameter and flow rate is well documented in the literature (Maeda 1996, Mchedlishvili and Maeda 2001, Lima et al 2009a, Garcia et al 2012, Bumseok et al 2014, however to the best of our knowledge the effect of a microbubble on the CFL thickness has never been investigated. The results from Figs.…”

A microfluidic device for partial cell separation and deformability assessment

“…A detailed description of the fabrication process can be found elsewhere [22,23]. Briefly, the microchannel geometry was drawn using Autocad, and a high resolution photomask was manufactured.…”