Microscopic corneal epithelial changes and clinical outcomes in simple limbal epithelial transplantation surgery after treatment with amniotic membrane eye drops (AMED): A case report

Bonacci, Erika; Kilian, Raphael; Rizzo, Clara; Gregorio, Alessandra De; Bosello, Francesca; Fasolo, Adriano; Ponzin, Diego; Marchini, Giorgio; Pedrotti, Emilio

doi:10.1016/j.ajoc.2022.101763

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…After centrifugation, supernatants may be sterilized by filtering through a 0.2-μm pore size membrane ( 245 , 246 ). Studies have shown that hAME used as eye drops (AMEED), supports proliferation and differentiation of corneal epithelial cells, enhances epithelial wound healing, and inhibits corneal neovascularization by supporting in vivo cultivation of limbal stem cells ( 244 – 249 ) whereby the extract is as efficient as AMT ( 250 ). Promising results are achieved for treatment of dry eye syndrome and Graft-versus-Host disease ( 251 – 253 ).…”

Section: Further Preparation Types and Application Fieldsmentioning

confidence: 99%

Preparation of human amniotic membrane for transplantation in different application areas

et al. 2023

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The human amniotic membrane (hAM) is the inner layer of the placenta and plays protective and nutritional roles for the fetus during pregnancy. It contains multiple growth factors and proteins that mediate unique regenerative properties and enhance wound healing in tissue regeneration. Due to these characteristics hAM has been successfully utilized in ophthalmology for many decades. This material has also found application in a variety of additional therapeutic areas. Particularly noteworthy are the extraordinary effects in the healing of chronic wounds and in the treatment of burns. But hAM has also been used successfully in gynecology, oral medicine, and plastic surgery and as a scaffold for in vitro cell culture approaches. This review aims to summarize the different graft preparation, preservation and storage techniques that are used and to present advantages and disadvantages of these methods. It shows the characteristics of the hAM according to the processing and storage methods used. The paper provides an overview of the currently mainly used application areas and raises new application possibilities. In addition, further preparation types like extracts, homogenates, and the resulting treatment alternatives are described.

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Section: Further Preparation Types and Application Fieldsmentioning

confidence: 99%

Preparation of human amniotic membrane for transplantation in different application areas

et al. 2023

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Spotlight on Amniotic Membrane Extract Eye Drops: A Review of the Literature

Kilian,

Bonacci,

Donner

et al. 2024

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

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Objective: To review the literature focusing on the effectiveness of amniotic membrane extract eye drops (AMEDs) in the treatment of ocular surface diseases. Methods: PubMed/MEDLINE, Scopus, and CENTRAL databases were searched until March 4, 2024. Overall, we identified 1,121 studies, 26 of which were selected for a full-text review. Twelve studies met the inclusion criteria and were analyzed for clinical improvements, time to resolution of corneal staining, adverse events, and preparation methods. Strength of clinical data was graded according to the Oxford Center for Evidence-Based Medicine. Results: Overall, AMED compounds were used in 296 eyes of 205 patients. Fifty-nine percent of eyes were treated for dry eye disease, 23% for an epithelial defect, and the rest (18%) for other corneal wound healing disorders. Three main types of eye drops preparation were described, that is, lyophilized, homogenized, and fresh AMED. Although the methods of outcome reporting were heterogeneous, all included studies showed various grades of improvement in both signs and symptoms. The overall incidence of ocular side effects was 2.3%. Conclusions: Despite the suboptimal quality of evidence, overall, the available literature suggests that AMED is a valuable tool in the treatment of ocular surface disorders.

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Microscopic corneal epithelial changes and clinical outcomes in simple limbal epithelial transplantation surgery after treatment with amniotic membrane eye drops (AMED): A case report

Cited by 2 publications

References 27 publications

Preparation of human amniotic membrane for transplantation in different application areas

Preparation of human amniotic membrane for transplantation in different application areas

Spotlight on Amniotic Membrane Extract Eye Drops: A Review of the Literature

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