Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

Khan, Muhammad Tanveer; Dalvin, Sussie; Nilsen, Frank; Male, Rune

doi:10.1194/jlr.m076430

Cited by 13 publications

(9 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vertebrates, there are three MTP transcript variants that have been identified both in humans and mice, which turned out to encode two MTP isoforms in mice but only a single translation product in humans (Mohler et al, 2007 ; Suzuki and Swift, 2016 ; Suzuki et al, 2016 ). In agreement with our findings, sequence differences between transcript variants of MTP genes are also exclusively present in the 5′ end region in these species (Mohler et al, 2007 ; Suzuki and Swift, 2016 ; Suzuki et al, 2016 ; Khan et al, 2017 ). These findings indicated that transcription of the MTP gene might be conserved across animal phyla.…”

Section: Discussionsupporting

confidence: 92%

“…According to transcriptome data, the three types of MTP transcript variants and/or MTP protein isoforms appear to be widely distributed in decapodan species ( Supplementary Figure 2 ). In fact, multiple MTP transcript variants and/or MTP protein isoforms are extensively observed in invertebrates and vertebrates (Mohler et al, 2007 ; Suzuki and Swift, 2016 ; Suzuki et al, 2016 ; Khan et al, 2017 ). For example, three MTP transcript variants were identified in the salmon louse Lepeophtheirus salmonis , and these were putatively translated into two MTP isoforms (Khan et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, multiple MTP transcript variants and/or MTP protein isoforms are extensively observed in invertebrates and vertebrates (Mohler et al, 2007 ; Suzuki and Swift, 2016 ; Suzuki et al, 2016 ; Khan et al, 2017 ). For example, three MTP transcript variants were identified in the salmon louse Lepeophtheirus salmonis , and these were putatively translated into two MTP isoforms (Khan et al, 2017 ). In vertebrates, there are three MTP transcript variants that have been identified both in humans and mice, which turned out to encode two MTP isoforms in mice but only a single translation product in humans (Mohler et al, 2007 ; Suzuki and Swift, 2016 ; Suzuki et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

dLp/HDL-BGBP and MTP Cloning and Expression Profiles During Embryonic Development in the Mud Crab Scylla paramamosain

et al. 2021

View full text Add to dashboard Cite

Lipids are the main energy source for embryonic development in oviparous animals. Prior to the utilization and catabolism, lipids are primarily transported from the yolk sac to embryonic tissues. In the present study, cDNA encoding a circulatory large lipid transfer protein (LLTP) superfamily member, the precursor of large discoidal lipoprotein (dLp) and high-density lipoprotein/β-1,3-glucan-binding protein (HDL-BGBP), named dLp/HDL-BGBP of 14,787 bp in length, was cloned from the mud crab Scylla paramamosain. dLp/HDL-BGBP was predicted to encode a 4,831 amino acids (aa) protein that was the precursor of dLp and HDL-BGBP, which were both detected in hemolymph by liquid chromatography–mass spectrometry (LC-MS/MS) analysis. For the intracellular LLTP, three microsomal triglyceride transfer protein (MTP) cDNAs of 2,905, 2,897, and 3,088 bp in length were cloned from the mud crab and were predicted to encode MTP-A of 881 aa, MTP-B of 889 aa, and MTP-C of 919 aa, respectively, which were different merely in the N-terminal region and shared an identical sequence of 866 aa. During embryonic development, the expression level of dLp/HDL-BGBP consecutively increased from the early appendage formation stage to the eye pigment-formation stage, which indicated that HDL-BGBP is probably the scaffolding protein for yolk lipid. For the MTP gene, MTP-C accounted for ~70% of MTP mRNA from the blastocyst stage to the nauplius stage, as well as the pre-hatching stage; MTP-C and MTP-A expression levels were comparable from the early appendage formation stage to the late eye pigment-formation stage; MTP-A was extremely low in blastocyst and gastrula stages; MTP-B was expressed at a relatively low-level throughout embryo development. The variations in the expression profiles among MTP transcripts suggested that MTP might play roles in the lipid droplet maturation and lipoprotein assembly during embryonic development.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

dLp/HDL-BGBP and MTP Cloning and Expression Profiles During Embryonic Development in the Mud Crab Scylla paramamosain

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Female lice were fixed overnight while larvae, ovaries and unfertilized eggs were fixed for 2 hrs. Oil Red O stain was performed according to the previously described method [ 48 ] with some modifications in the length of time when adult lice were stained. After fixation, all samples were washed three times with ice-cold PBS and resuspended in 60% isopropanol for 10–30 minutes.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of the lipophorin receptor in the crustacean ectoparasite Lepeophtheirus salmonis

et al. 2018

Self Cite

View full text Add to dashboard Cite

The Salmon louse (Lepeophtheirus salmonis) is a marine ectoparasite of salmonid fish in the Northern Hemisphere and considered as a major challenge in aquaculture and a threat to wild populations of salmonids. Adult female lice produce a large number of lipid-rich eggs, however, the mechanism of maternal lipid transport into developing eggs during salmon louse reproduction has not been described. In the present study, a full-length L. salmonis lipophorin receptor (LsLpR) consisting of 16 exons was obtained by RACE and RT-PCR. The predicted ORF was 952 amino acids and structural analysis showed five functional domains that are similar to LpR of insects and decapods. Phylogenetic analysis placed the LsLpR together with LpRs from decapods and insects. Expression analysis revealed that the relative abundance of LsLpR transcripts was highest in the larvae and adult female lice. In adult females, the LsLpR transcripts and protein were found in the ovary and vitellogenic oocytes whereas, in larvae, the LsLpR transcripts were found in the neuronal somata of the brain and the intestine. Oil Red O stain results revealed that storage of neutral lipids was found in vitellogenic oocytes and ovaries of adult females, and in the yolk of larvae. Moreover, RNA interference (RNAi) was conducted to demonstrate the function of LsLpR in reproduction and lipid metabolism in L. salmonis. In larvae, the transcription of LsLpR was decreased by 44–54% while in an experiment LsLpR knockdown female lice produced 72% less offspring than control lice.

show abstract

“…RNA interference screens have aided the study of hostparasite interactions, host recognition (Komisarczuk et al, 2017;Núñez-Acuña et al, 2019), and physiology at various life stages of lice (Sandlund et al, 2016(Sandlund et al, , 2018Komisarczuk et al, 2018Komisarczuk et al, , 2020Braden et al, 2020;Guragain et al, 2020). RNAi has also facilitated in the functional studies of key genes involved in lice reproduction (Dalvin et al, 2009;Tröße et al, 2014;Eichner et al, 2015a;Khan et al, 2017;Borchel and Nilsen, 2018;Borchel et al, 2019;Heggland et al, 2019a,b). In addition, the method is used to identify the potential vaccine targets in C. rogercresseyi (Carpio et al, 2011;Maldonado-Aguayo and Gallardo-Escárate, 2014).…”

Section: Rna Interferencementioning

confidence: 99%

Principles and Methods of Counteracting Harmful Salmon–Arthropod Interactions in Salmon Farming: Addressing Possibilities, Limitations, and Future Options

et al. 2021

View full text Add to dashboard Cite

The arthropod salmon louse (Lepeophtheirus salmonis) is a major threat to Atlantic salmon aquaculture and wild salmonids. Essentially like in monoculture, very high concentrations of susceptible hosts may result in high reproduction and severe production of waves of pests. Pest management is crucial both for fish health and protection of wild fish populations from aquaculture influence. Various methods have been utilized to control salmon lice infestations, such as pesticide use, physical treatments, construction modifications, fallowing, breeding, vaccination, and biological control. Most of the methods are partially successful, but none completely fulfills the necessary pest control strategy. Like in agriculture, lice/pest management is an arms race, but the marine environment makes it even more difficult to precisely hit the target pest and avoid unintended negative effects on general wildlife. In this study, we provide an overview of the methods and principles of salmon lice management and address current possibilities and limitations. We also highlight the potential of emerging strategies and enabling technologies, like genome editing, RNA interference, and machine learning, in arthropod management in aquaculture.

show abstract

Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis)

Cited by 13 publications

References 58 publications

dLp/HDL-BGBP and MTP Cloning and Expression Profiles During Embryonic Development in the Mud Crab Scylla paramamosain

dLp/HDL-BGBP and MTP Cloning and Expression Profiles During Embryonic Development in the Mud Crab Scylla paramamosain

Molecular characterization of the lipophorin receptor in the crustacean ectoparasite Lepeophtheirus salmonis

Principles and Methods of Counteracting Harmful Salmon–Arthropod Interactions in Salmon Farming: Addressing Possibilities, Limitations, and Future Options

Contact Info

Product

Resources

About