Microsomal triglyceride transfer protein regulates intracellular lipolysis in adipocytes independent of its lipid transfer activity

Unmasking crucial residues in adipose triglyceride lipase for coactivation with comparative gene identification-58

“…The stimulatory effect of CGI-58 on ATGL is observed upon direct interaction of both proteins and demonstrates the highest efficiency at approximately equimolar concentrations of enzyme and activator protein ( 7 ). ATGL is inhibited by the proteins G0/G1 switch gene 2 (G0S2), hypoxia-inducible lipid droplet-associated protein (HILPDA) and the very recently described microsomal triglyceride transfer protein ( 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ). It is assumed that CGI-58 and G0S2 interact at different binding sites: Lack of ATGL-activity in G0S2-expressing cells could not be rescued with increased CGI-58 concentrations ( 18 ).…”

Intestinal Reg4 deficiency confers susceptibility to high-fat diet-induced liver steatosis by increasing intestinal fat absorption in mice

“… 27 , 28 The proteins MTTP and TIP47 are responsible for triglyceride chylomicron packaging and formation. 29 , 30 Reg4 ΔIEC mice here had an increase in the protein level of the main components responsible for intestinal fat absorption during feeding with a HFD. Although the human intestinal epithelial cells FHs 74 Int had reduced expression of these proteins after treated with REG4 protein.…”

Intracellular lipase and regulation of the lipid droplet

“…The primary function of MTP is to associate triglyceride, phospholipid, and cholesterol with apolipoprotein B (apoB) to create chylomicrons and VLDL. Adipose specific MTP knockout mice were reported to have a reduction in adipose tissues, which appears to be due to less MTP association with the lipid droplet and increased ATGL activation [44 ▪ ].…”