Microspore embryogenesis in vitro: the role of stresses

Djatchouk, T. I.; Khomyakova, O. V.; Akinina, V. N.; Kibkalo, I. A.; Pominov, A. V.

doi:10.18699/vj19.466

Cited by 9 publications

(2 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Одной из самых востребованных технологий для ускорения процесса получения гибридов F 1 капустных культур является производство линий дигаплоидов в культуре изолированных микроспор (Domblides et al, 2018;Djatchouk et al, 2019;Dong et al, 2021). Данная технология ускоряет получение генетически стабильных линий, позволяет сочетать различные признаки в одном генотипе и облегчает поиск редких признаков, контролируемых рецессивными аллелями генов (Ferrie, Caswell, 2011).…”

Section: Introductionunclassified

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

Курина

Artemyeva

2023

Biotehnologiâ i selekciâ rastenij

View full text Add to dashboard Cite

Basic and applied scientific research in plant cell technologies contribute to the successful development of agricultural plant breeding, which allows the creation of new forms of plants 2-4 times faster than by traditional breeding methods. To obtain inbred lines of most vegetable crops, about 5-7 cycles of self-pollination are required. As a result, the creation of a new cultivar/hybrid takes more than 10-12 years on an average. To successfully create a variety or hybrid, it is necessary to select parental pairs in the form of inbred lines. The VIR collection of vegetables and cucurbit crops includes 52,889 accessions, representatives of 29 families, 145 genera, and 610 species. The use of biotechnological methods is an important direction for accelerating the breeding of vegetable crops. Due to the relevance of introducing cell technologies into the breeding programs of the VIR Department of Genetic Resources of Vegetable and Cucurbit Crops, a Laboratory of Breeding and Cell Technologies was set up in 2022. The goal of the research to be performed at the new laboratory is to accelerate the creation of source material, cultivars and hybrids by combining traditional breeding methods and cell technologies. The objects of the study include cultivated forms and wild relatives of cabbage Brassica oleracea L., turnip Brassica rapa L., lettuce Lactuca L., tomato Lycopersicon Mill and vegetable sweet corn Zea mays var. saccharata Sturt. In the present review, we consider the main results of breeding cabbage, tomato, and lettuce which have been obtained through applying cell technologies. Despite the progress obtained, there are still several problems in this area. The lack of standardized, efficient and reproducible protocols for in vitro methods often hinders their practical use. The tasks facing the laboratory in creating the initial breeding material and new cultivars and hybrids with the use of both conventional methods and cell technologies are relevant and correspond to the world level.

show abstract

Section: Introductionunclassified

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

Курина

Artemyeva

2023

Biotehnologiâ i selekciâ rastenij

View full text Add to dashboard Cite

show abstract

“…The isolated microspore culture technique produces more regenerates compared to those of unfertilized seedbuds and anthers and is widely applied, especially in the cabbage family (Djatchouk et al, 2019;Kozar et al, 2020). Moreover, this technique excludes the somatic cells of a donor plant from the growing medium, leaving no doubt about the regenerates' origin.…”

Section: Introductionmentioning

confidence: 99%

Association of bud and anther morphology with developmental stages of the male gametophyte of melon (Cucumis melo L.)

et al. 2022

View full text Add to dashboard Cite

Correlations between the morphological features of f lower buds and the developmental stages of the male gametophyte are of great practical interest as a reliable marker that accelerates and simplif ies the selection of appropriate plant material for isolated microspore culture. Microspore culture enables one to quickly obtain many pure lines of different vegetable crops, but it has not yet been widely applied in the melon (Cucumis melo L.). To successfully apply this technique in a new culture, one has to optimize many of its elements: f irst, f ind the biological markers for selecting the f lower buds containing the microspores of certain development stages. The paper presents the results of research estimating the correlations between the length and diameter of the f lower buds, the length of the visual part of the corolla, the length of the anthers and the development stages of the male gametophyte in the F1 hybrid of the Kim Hong Ngoc melon. The strongest correlation (CC = 0.885) was found for the f lower bed diameter and a strong correlation (CC = 0.880), for the bud length. The corolla’s visual part was a less reliable morphological feature, and the anther’s length should not be used as a parameter to predict the developmental stages of the melon’s male gametophyte. It was also found that one anther could contain the microspores and pollen grains of different developmental stages. In the f lower buds less than 4 mm in length and 1.51 ± 0.02 mm in diameter prevailed tetrads, and in the buds 4.0–4.9 mm in length and 2.30 ± 0.02 mm in diameter, early microspores. The microspores of a middle stage of development prevailed in the f lower buds 5.0–5.9 mm in length and 2.32 ± 0.00 mm in diameter; mid and late vacuolated microspores, in the buds 6.0–8.9 mm in length and 2.96 ± 0.37 mm in diameter; and two-celled pollen, in the buds more than 9 mm in length and more than 3.97 ± 0.34 mm in diameter.

show abstract

Efficiency of Androgenesis In Vitro in the Culture of Isolated Winter Wheat Anthers: 1. The Influence of the Duration of Low-Temperature Pretreatment of Donor Plants on Respiration, Carbohydrate Content, and ROS Levels in Flowers from Different Parts of the Spike

Lyubushkina,

Polyakova,

Pomortsev

et al. 2023

Russ J Plant Physiol

View full text Add to dashboard Cite

Microspore embryogenesis in vitro: the role of stresses

Cited by 9 publications

References 72 publications

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

Association of bud and anther morphology with developmental stages of the male gametophyte of melon (Cucumis melo L.)

Efficiency of Androgenesis In Vitro in the Culture of Isolated Winter Wheat Anthers: 1. The Influence of the Duration of Low-Temperature Pretreatment of Donor Plants on Respiration, Carbohydrate Content, and ROS Levels in Flowers from Different Parts of the Spike

Contact Info

Product

Resources

About