Microspore Embryogenesis Induced by Low Gamma Dose Irradiation in Apple

Abstract

Flower buds and extracted anthers of two apple genotypes, 'Golden Delicious' -a previously known non-androgenic genotype -, and 'Topred' -a known androgenic genotype -, were irradiated by gamma-rays at doses ranging from 5 to 20 Gy, after 0 to 5 weeks of cold pre-treatment (3 °C). When the extracted anthers were irradiated, both microspore embryogenesis and callogenesis were limited for the two genotypes. For 'Golden Delicious', androgenic embryogenesis was induced from the flower buds irradiated at 10 to 20 Gy and after 3 to 5 weeks of cold pre-treatment. For 'Topred', androgenic embryogenesis was slightly improved after flower bud irradiation at 5 to 10 Gy following cold pre-treatment from 0 to 3 weeks; it was limited after 5 weeks of cold pre-treatment.

Doubled haploid production in fruit crops

“…Erovan with pollen irradiated at 500-1000 Gy, followed by in vitro embryo culture (Zhang, 1988). This technique has been successfully applied to other apple cultivars also with different c-rays from Cobalt 60 (Zhang et al, 1987;Zhang and Lespinasse, 1991;Zhang et al, 1992;De Witte and Keulemans, 1994).…”