Microstructural and histochemical advances on the digestive gland of the common cuttlefish, Sepia officinalis L.

Costa, Pedro M.; Rodrigo, Ana P.; Costa, Maria Helena

doi:10.1007/s00435-013-0201-8

Cited by 28 publications

(30 citation statements)

References 38 publications

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“…At the fragmentation phase, digestive cells release digestive end products, in the form of membrane‐bound spherules, into the lumen of the tubules. Such spherules are similar to those described by Costa et al () for the common cuttlefish S. officinalis , whose digestive epithelia share some similarities with gastropods.…”

Section: Discussionsupporting

confidence: 87%

“…The differential strength of the PAS reaction reveals the accumulation of sugars as it has been observed for other mollusks like the common cuttlefish, Sepia officinalis Linnaeus, 1758 (Costa, Rodrigo, & Costa, 2013) and the carnivorous gastropod Adelomelon beckii (Broderip, 1836) . In the digestive cells of both carnivorous and herbivorous cephalaspideans, Lobo-da-Cunha, Alves, Oliveira, Guimarães, and…”

Section: Cell Type Composition Of the Digestive Glandmentioning

confidence: 57%

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Morphology of the digestive gland of the marine panpulmonate limpetSiphonaria lessonii: A cytological, histochemical, and ultrastructural description

Landro

Teso

Arrighetti

2019

Journal of Morphology

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The molluskan digestive gland has been widely studied and its structural and ultrastructural descriptions have allowed the understanding of its several functions. Despite siphonarids are broadly distributed around the world, morphological studies on their digestive system are poorly represented. The panpulmonate limpet Siphonaria lessonii is the most abundant gastropod and the dominant herbivore in the rocky intertidal coast of Buenos Aires. The aim of this study was to describe the morphology, histology, ultrastructure, and histochemistry of the digestive gland of this gastropod as well as the cycle of activity of digestion. For that, different histochemical techniques along with light microscopy, transmission electron microscopy, and scanning electron microscopy were employed. This study revealed a complex epithelium, composed of a simple layer with five cell types. Digestive cells and vacuolated cells are responsible for intracellular digestion and energy accumulation; basophilic cells, secrete substances that would be involved in extracellular digestion; pigmented cells might have an excretory function and thin cells would correspond to undifferentiated cells. In addition, the tubules present a changing morphology according to the digestive activity that they undergo. As S. lessonii is a grazer that feeds continuously, the cycle of activity of the digestive gland seems to be daily.

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Section: Discussionsupporting

confidence: 87%

Section: Cell Type Composition Of the Digestive Glandmentioning

confidence: 57%

Morphology of the digestive gland of the marine panpulmonate limpetSiphonaria lessonii: A cytological, histochemical, and ultrastructural description

Landro

Teso

Arrighetti

2019

Journal of Morphology

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“…Numerous authors have studied the digestive gland of cephalopods (Bidder, ; Boucaud‐Camou & Boucher‐Rodoni, ; Boucaud‐Camou & Yim, ; Costa, Rodrigo, & Costa, ; Semmens, ; Semmens, Moltschaniwsky, & Alexander, ; Swift et al., ). Most of these studies focus on structural changes in the digestive cells and the abundance of inclusions during the digestive process (Bidder, ; Boucaud‐Camou & Boucher‐Rodoni, ; Boucaud‐Camou & Yim, ; Semmens, ; Semmens et al., ).…”

Section: Discussionmentioning

confidence: 99%

Anatomical and histochemical features of the digestive system ofOctopus vulgarisCuvier, 1797 with a special focus on secretory cells

2018

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This study reports a detailed anatomical and histological study of the digestive system of Octopus vulgaris. Emphasis was placed on characterising the glands and glandular cells and their distribution throughout the digestive tract. The use of classic histological and histochemical techniques revealed two morphological types of glandular cells: granular and mucous. Moreover, the histochemical analysis indicated specialisation of mucous glandular cells in the buccal mass, the submandibular gland and the caecum for secreting acid and neutral glycoconjugates. The cells of the anterior salivary glands are specialised for secreting neutral glycoproteins, and those of the posterior salivary glands are specialised for granular and mucous secretion. The oesophagus, crop and stomach lack glandular cells, but both granular and mucous glandular cells are found in the intestine. An unusual structure resembling the typhlosole of bivalves is described for the first time in the intestine of O. vulgaris. The highly ciliated epithelium and location of the structure in the anterior part of the intestine suggest a possible role in bypassing the caecum, stomach and intestine. We discuss how these cells and organs contribute to the process of digestion in the light of the present histological and histochemical data and of previously published information on the morphology and physiology of digestion in the octopus.

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“…Histological and histochemical analyses, both in paraffin and in resin sections, were performed using a tetrachrome (TC) technique based on Alcian Blue (AB) for acidic sugars (pH 0.5-2.5 to indicate sulphated and carboxylated mucins, respectively), Periodic Acid/ Schiff's (PAS) for neutral polysaccharides, Weigert's iron Haematoxylin (WH) for chromatin, and Picric Acid (PA) for muscle and cytoplasm, following Costa & Costa (2012), with modifications (Costa et al 2014;Rodrigo et al 2015). Resin sections required permeabilisation using a saturated solution of potassium hydroxide in ethanol 100% for 5 min followed by 2 min of 2% m/v borax (sodium borate) just before staining.…”

Section: Methodsmentioning

confidence: 99%

A morphoanatomical approach to the adaptive features of the epidermis and proboscis of a marine Polychaeta: Eulalia viridis (Phyllodocida: Phyllodocidae)

et al. 2018

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Eulalia viridis is a marine Polychaeta of the rocky intertidal that, despite its simple anatomy, is an active predator of much larger invertebrates, from which it extracts pieces of soft tissue through suction. This uncanny feeding strategy triggered the pursuit for the morphological mechanisms that enable adaptation to its environment. The evaluation of the worm anatomy and microanatomy, combining electron and optical microscopy, revealed a series of particular adaptations in the epidermis and in the proboscis (the heavily muscled eversible pharynx). Besides its function in feeding, the proboscis is the main sensory organ, being equipped with numerous sensorial papillae holding chemoreceptors. Additionally, the proboscis possesses tentacles that become exposed when the organ is everted. These provide fast release of mucus and toxins, from mucocytes and special serous cells, respectively (the latter involving both merocrine and apocrine processes), whenever contact with a prey occurs. In its turn, the epidermis provides protection by cuticle and mucus secretion and has a sensorial function that may be associated to the worm's uncommon green pigment cells. Eulalia viridis presents a series of elegant adaptive tools to cope with its environment that are evolutionarily designed to counterbalance its relatively simple body plan.

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Microstructural and histochemical advances on the digestive gland of the common cuttlefish, Sepia officinalis L.

Cited by 28 publications

References 38 publications

Morphology of the digestive gland of the marine panpulmonate limpetSiphonaria lessonii: A cytological, histochemical, and ultrastructural description

Morphology of the digestive gland of the marine panpulmonate limpetSiphonaria lessonii: A cytological, histochemical, and ultrastructural description

Anatomical and histochemical features of the digestive system ofOctopus vulgarisCuvier, 1797 with a special focus on secretory cells

A morphoanatomical approach to the adaptive features of the epidermis and proboscis of a marine Polychaeta: Eulalia viridis (Phyllodocida: Phyllodocidae)

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