Microstructural constitutive model of active coronary media

Chen, Huan; Luo, Tong; Zhao, Xuefeng; Lu, Xiao; Huo, Yunlong; Kassab, Ghassan S.

doi:10.1016/j.biomaterials.2013.06.035

Cited by 40 publications

(77 citation statements)

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“…We have recently provided a comprehensive study of vessel wall microstructure, including measurement of geometries of elastin and collagen fibers (3), along with their microstructural deformation (3,6), to integrate the data into a microstructural model of coronary media (5). Here, we validated a 3D microstructural constitutive model for the coronary adventitia based on measured microstructural features.…”

Section: Discussionmentioning

confidence: 99%

“…The media consists of concentric smooth muscle cell layers, elastin lamellae, collagen fibril bundles, and a network of elastic fibrils, serving as the most important mechanical layer at physiological conditions. Chen et al (5) recently developed a microstructural model, including measured microstructural data and active properties of smooth muscle cells, to predict accurately biaxial vasoactivity of coronary artery media. A future study that integrates the media with the adventitia model is needed to provide a two-layer microstructural model of an intact coronary artery.…”

Section: Discussionmentioning

confidence: 99%

“…At higher pressures (e.g., hypertension), collagen fibers reach their straightened lengths, and the adventitia becomes a stiff tube to prevent the media from overstretch and rupture (3,6,44,48). Smooth muscle cells in media predominate active responses of blood vessels, whereas elastin and collagen fibers only contribute to passive mechanical behaviors (5,21). The mechanical properties of individual fibers have been broadly studied in literature (1,13,15,20,44,46), and microstructural parameters and deformation in coronary adventitia have been quantitatively measured in our recent studies (3,6).…”

mentioning

confidence: 99%

“…The first ones considered the vessel wall as a composite of elastin and collagen fibers embedded in a fluid-like matrix (5,9,17,24,25,45). The fibers are the only constituent phases that sustain nonhydrostatic loading, such as tension and shear, whereas the contribution of the fluid-like matrix is only a hydrostatic pressure.…”

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confidence: 99%

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A validated 3D microstructure-based constitutive model of coronary artery adventitia

et al. 2016

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Chen H, Guo X, Luo T, Kassab GS. A validated 3D microstructure-based constitutive model of coronary artery adventitia. J Appl Physiol 121: 333-342, 2016. First published May 12, 2016 doi:10.1152/japplphysiol.00937.2015.-A structure-based model that accurately predicts micro-or macromechanical behavior of blood vessels is necessary to understand vascular physiology. Based on recently measured microstructural data, we propose a three-dimensional microstructural model of coronary adventitia that incorporates the elastin and collagen distributions throughout the wall. The role of ground substance was found to be negligible under physiological axial stretch z ϭ 1.3, based on enzyme degradation of glycosaminoglycans in swine coronary adventitia (n ϭ 5). The thick collagen bundles of outer adventitia (n ϭ 4) were found to be undulated and unengaged at physiological loads, whereas the inner adventitia consisted of multiple sublayers of entangled fibers that bear the majority of load at higher pressures. The microstructural model was validated against biaxial (inflation and extension) experiments of coronary adventitia (n ϭ 5). The model accurately predicted the nonlinear responses of the adventitia, even at high axial force (axial stretch ratio z ϭ 1.5). The model also enabled a reliable estimation of material parameters of individual fibers that were physically reasonable. A sensitivity analysis was performed to assess the effect of using mean values of the distributions for fiber orientation and waviness as opposed to the full distributions. The simplified mean analysis affects the fiber stress-strain relation, resulting in incorrect estimation of mechanical parameters, which underscores the need for measurements of fiber distribution for a rigorous analysis of fiber mechanics. The validated structure-based model of coronary adventitia provides a deeper understanding of vascular mechanics in health and can be extended to disease conditions. constitutive model; collagen; elastin; microstructure; adventitia; material parameters MICROSTRUCTURAL APPROACHES have been advocated for modeling blood vessels to understand better the nonlinear mechanical responses of vessels (7,17,18,25). Mechanical predictions are thought to be more accurate than those of phenomenological models, as these models account for microstructural features of vessel components, as well as heterogeneity of material properties. The microstructural parameters have been ad hoc rather than based on experimental measurements, due to limited morphological data of the vessel wall (4,17,23,51). The microstructure of the vessel wall, which includes elastin and collagen fibers, smooth muscle cells, and ground substance, is distributed differently in individual vessel layers, i.e., tunica media and adventitia (8,36,43). A microstructural constitutive model should be specific for a particular layer based on experimental measurements of microstructure (2).The coronary adventitia consists of three major constituents-elastin, collagen fibers, and ground substance-and...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A validated 3D microstructure-based constitutive model of coronary artery adventitia

et al. 2016

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“…Microstructure-based models are both popular as well as useful tools that include mechanical properties as well as the morphology of the tissue component to explain the overall mechanical behavior of the tissue (Lanir 1983;Holzapfel and Ogden 2010;Chen et al 2013). The contribution of VSMCs is crucial for modeling active behavior (such as vascular constriction).…”

Section: Relevance Of the Results To The Biomechanicsmentioning

confidence: 99%

Segmental differences in the orientation of smooth muscle cells in the tunica media of porcine aortae

Tonar

Kochová

Cimrman

et al. 2014

Biomech Model Mechanobiol

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The orientation of vascular smooth muscle cells of porcine aortae was assessed to test the widely accepted assumption that these smooth muscle cells are arranged in two helices. We used tangential histological sections of 82 samples of five anatomical segments of thoracic and abdominal porcine aortae and three age groups in animals ranging in age from 5 to 210 days. The distribution of the orientation of smooth muscle cell nuclei in five proximodistal segments of the porcine aortae was determined using an algorithm that fitted a mixture of one to five von Mises probability distributions of the data retrieved from histological micrographs. Automated tracking of the nuclei was confirmed by and consistent with manual histological analysis. The orientation of the vascular smooth muscle cells was successfully fitted using two von Mises distributions in most of the samples with different ages, wall thicknesses, and anatomical positions, which corresponds to two populations of vascular smooth muscle cells. A minor fraction of samples also required a tertiary von Mises distribution to describe the orientation of the smooth muscle cell nuclei. The distribution of vascular smooth muscle cells in five aortic segments ranging from the thoracic ascending aorta to the abdominal intrarenal aorta exhibited similar main directions but different shapes. These results are consistent with the widely used model of two muscular helices intermingling in the arterial wall. Furthermore, we calculated the central angles of symmetry and the mean value of angles between the two assumed smooth muscle directions. We also successfully approximated the orientation of the smooth muscle cells using a mixture of von Mises distributions and our open-source software named dist_mixtures. This method is openly available to researchers who are interested in mathematically assessing the orientation of cell nuclei in various tissues.

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Chemical imaging of fresh vascular smooth muscle cell response by epi‐detected stimulated Raman scattering

Zhang

Chen

et al. 2017

Journal of Biophotonics

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An understanding of deformation of cardiovascular tissue under hemodynamic load is crucial for understanding the health and disease of blood vessels. In the present work, an epi-detected stimulated Raman scattering (epi-SRS) imaging platform was designed for in situ functional imaging of vascular smooth muscle cells (VMSCs) in fresh coronary arteries. For the first time, the pressure-induced morphological deformation of fresh VSMCs was imaged with no fixation and in a label-free manner. The relation between the loading pressure and the morphological parameters, including angle and length of the VSMCs, were apparent. The morphological responses of VMSCs to drug treatment were also explored, to demonstrate the capability of functional imaging for VSMCs by this method. The time-course imaging revealed the drug induced change in angle and length of VSMCs. The present study provides a better understanding of the biomechanical framework of blood vessels, as well as their responses to external stimulations, which are fundamental for developing new strategies for cardiovascular disease treatment.

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Microstructural constitutive model of active coronary media

Cited by 40 publications

References 40 publications

A validated 3D microstructure-based constitutive model of coronary artery adventitia

A validated 3D microstructure-based constitutive model of coronary artery adventitia

Segmental differences in the orientation of smooth muscle cells in the tunica media of porcine aortae

Chemical imaging of fresh vascular smooth muscle cell response by epi‐detected stimulated Raman scattering

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