Microvascular cells: A special focus on heterogeneity of pericytes in diabetes associated complications

Rajendran, Sharmila; Shanmuganathan, S; Dharmarajan, Arun; Kuppan, Kaviarasan

doi:10.1016/j.biocel.2021.105971

Cited by 17 publications

(11 citation statements)

References 73 publications

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“…Retinal pericytes are important constituents of the inner blood-retinal barrier (BRB); they are located at the basement membrane of retinal capillaries and tightly wrap around endothelial cells, keeping the integrity and stability of the BRB and regulating retinal blood flow and angiogenesis as well [ 1 ]. The ratio of pericytes to endothelial cells in the retina is relatively high (1 : 1), compared with capillaries in other tissues [ 2 ], indicating the importance of pericytes in maintaining retinal functions. Selective pericytes loss is thought to be a hallmark of early diabetic retinopathy (DR) and also contributing to many clinical characteristics of DR.…”

Section: Introductionmentioning

confidence: 99%

Autophagy Modulates the Migration of Retinal Pericytes Induced by Advanced Glycation End Products

Lin

Zhou

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Retinal pericyte migration occurs in the early stage of diabetic retinopathy (DR), which is one of the important causes of pericyte loss. Autophagy has been found to play essential roles in the regulation of many types of cell migration. In this study, we explored the relationship between autophagy and retinal pericyte migration. In diabetic rats, the retinas became thinner, and the level of autophagy in each cell layer increased. In the primary culture of bovine retinal pericytes, we found that advanced glycation end products (AGEs) increased the migratory cell ability without influencing cell viability, which also increased the phosphorylation of focal adhesion kinase (FAK) and the expression of matrix metalloproteinase (MMP)-2 and decreased the expression of vinculin. AGEs-induced retinal pericyte autophagy and the inhibition of autophagy with chloroquine significantly inhibited cell migration, reversed AGEs-induced FAK phosphorylation, and changed vinculin and MMP-2 protein expression. These results provide a new insight into the migration mechanism of retinal pericytes. The early control of autophagy has a potential effect on regulating pericyte migration, which may contribute to keeping the integrity of retinal vessels in DR.

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Section: Introductionmentioning

confidence: 99%

Autophagy Modulates the Migration of Retinal Pericytes Induced by Advanced Glycation End Products

Lin

Zhou

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…An increase of soluble pericyte marker in the cerebrospinal fluid was found parallel to the BBB leakage, suggesting the critical role of pericyte in maintaining BBB integrity ( 26 ). In T2DM, loss of pericyte is an early manifestation of diabetic retinopathy ( 27 ). In addition, the normal cellular function of the cerebral pericyte was impaired by T2DM ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal Dynamics of Cerebral Vascular Permeability in Type 2 Diabetes-Related Cerebral Microangiopathy

Chen

Shu

et al. 2022

Front. Endocrinol.

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AimsDiabetes-related cerebral microangiopathy can manifest as cerebral small vessel disease (CSVD) and exhibit cognitive decline. To find the early change of function in advance, this study examined the spatiotemporal dynamics of cerebral vascular permeability (Ktrans) in the progression of type 2 diabetes mellitus (T2DM).MethodsKtrans was cross-sectionally measured in T2DM and non-diabetes groups with or without CSVD using dynamic contrast-enhanced MRI (DCE-MRI).ResultsIn all patients with T2DM, the Ktrans of white matter (WM) was increased, whereas the Ktrans of gray matter (GM) was increased only in T2DM with CSVD. The involvement of WM was earlier than GM and was before the CSVD features could be visualized on MRI. Among the commonly available four CSVD items of MRI, microbleeds were the most sensitive, indicating the increased permeability in all patients. Increased Ktrans in T2DM was more associated with moderate WM hyperintensity but less with the presence of lacunae or multiple perivascular spaces, in contrast to patients without diabetes. The differential correlation suggested distinct mechanisms underlying diabetes-related CSVD and other CSVDs.ConclusionsThis study highlights the early development of cerebral microangiopathy with increased BBB leakage in T2DM, before the CSVD features can be visualized on MRI. The results may increase the proactivity of clinicians in recognizing the subsequent neurological comorbidities.

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“…Pericytes are important components of arterioles, veins, capillaries ( 31 ), providing support for endothelial cells ( 32 ). Within the vascular structure, endothelial cells surround to form a lumen.…”

Section: Discussionmentioning

confidence: 99%

The Anti-Colon Cancer Effects of Essential Oil of Curcuma phaeocaulis Through Tumour Vessel Normalisation

et al. 2021

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BackgroundNormalising tumour vessels had become a significant research focus in tumour treatment research in recent years. Curcumae rhizoma (CR) is an essential plant in traditional Chinese medicine as it promotes blood circulation and removes blood stasis. Similarly, CR improves local blood circulation.PurposeWe explored the anti-colon cancer effects of essential oil from CR (OCR) by investigating its role in normalising tumour vessels. We also provided a basis for research and development into new anti-cancer drugs.MethodsWe used colon cancer as a research focus to investigate OCR. We established an in vitro co-culture model of colon cancer cells and human umbilical vein endothelial cells (HUVEC). We also established an in vivo subcutaneous implant colon cancer model in nude mice. These studies allowed us to evaluate the comprehensive effects of OCR in in vivo and in vitro colon cancer and its role in normalising tumour blood vessels.ResultsIn vitro, we found that OCR inhibited Human colon cancer cells (HCT116) and HUVEC cell proliferation and inhibited vascular endothelial growth factor-a (VEGFa) mRNA and protein expression in HUVECs in a co-culture system. Our in vivo studies showed that OCR inhibited colon cancer tumour growth, reduced angiogenesis in tumours and increased vascular endothelial (VE)-cadherin and pericyte coverage in tumour vessels.ConclusionsOCR inhibited colon cancer growth both in in vivo and in vitro models, reduced angiogenesis in tumours, improved tumour vessel structures and normalised tumour vessels.

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Microvascular cells: A special focus on heterogeneity of pericytes in diabetes associated complications

Cited by 17 publications

References 73 publications

Autophagy Modulates the Migration of Retinal Pericytes Induced by Advanced Glycation End Products

Autophagy Modulates the Migration of Retinal Pericytes Induced by Advanced Glycation End Products

Spatiotemporal Dynamics of Cerebral Vascular Permeability in Type 2 Diabetes-Related Cerebral Microangiopathy

The Anti-Colon Cancer Effects of Essential Oil of Curcuma phaeocaulis Through Tumour Vessel Normalisation

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