Microwave assisted alkali treated wheat straw as a substrate for co-production of (hemi)cellulolytic enzymes and development of balanced enzyme cocktail for its enhanced saccharification

Patel, Harshvadan; Divecha, Jyoti; Shah, Amita

doi:10.1016/j.jtice.2016.12.032

Cited by 21 publications

(4 citation statements)

References 37 publications

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“…Therefore, direct comparison of enzyme loading among different reports was not available. Even so, enzyme loading (8.1 FPU/gds) in this work was relatively lower than 41.28 FPU/gds (Sheng et al 2021), 35.0 FPU/gds (Zheng et al 2018), 38.2 FPU/gds (Zhao et al 2017, 20.0 FPU/gds (Momayez et al 2019), 10.0 FPU/gds (Patel et al 2017) and 53.9 FPU/gds (Wu et al 2018) in the other seven previous reports. Lower enzyme loading can reduce cellulases input cost for hydrolysis.…”

Section: Optimization Of Enzymatic Hydrolysis Of the Pretreated Wheat...contrasting

confidence: 54%

Optimization of potassium hydroxide combined urea pretreatment and enzymatic hydrolysis of wheat straw using response surface methodology for improving sugar production

Zhang,

2023

BioRes

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To improve sugar yields from wheat straw (WS), response surface methodology (RSM) was adopted to optimize potassium hydroxide combined urea pretreatment and enzymatic hydrolysis of WS. Glucose and xylose yields from the pretreated WS were used as responses during the whole optimization. Potassium hydroxide concentration, time and temperature during pretreatment were found to have significant effects on sugar yields. Sugar yields could be enhanced while WS was pretreated using 45 g/L potassium hydroxide solution containing 15 g/L urea with solid to liquid ratio of 1:5 (g/mL) at 74.0 °C for 50 min. Cellulose recovery, hemicellulose recovery, and lignin removal after optimization were 98.1%, 72.6%, and 75.8%, respectively. In addition, enzyme loading, biomass loading, and reaction time during enzymatic hydrolysis also had significant effects on sugar yields. Maximal yields of glucose (610.25 mg/gds, miligram per gram dry substrate) and xylose (221.26 mg/gds) could be achieved while hydrolysis was carried out at 50 °C for 32.8 h with 141 g/L of biomass loading, 8.1 FPU/gds (filter paper activity unit per gram dry substrate) of enzyme loading and 0.4% (w/v) of polysorbate 80. The corresponding cellulose conversion and hemicellulose conversion were 97.2% and 90.4%, respectively.

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Section: Optimization Of Enzymatic Hydrolysis Of the Pretreated Wheat...contrasting

confidence: 54%

Optimization of potassium hydroxide combined urea pretreatment and enzymatic hydrolysis of wheat straw using response surface methodology for improving sugar production

Zhang,

2023

BioRes

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“…The adoption of lower enzyme loading could reduce the enzyme input cost. Though 10.0 FPU/g ds adopted by Patel et al (2017) [39] was lower than that (10.8 FPU/g ds ) in this work, the adoption of commercial cellulases in that work could enhance the enzyme input cost. In addition, the cellulose and hemicellulose conversions (94.0%, 83.5%) in this work were the most competitive among the reports in Table 6.…”

Section: Optimization Of Enzymatic Hydrolysis Of the Pretreated Wheat...mentioning

confidence: 71%

Optimization of Alkaline Hydrogen Peroxide Pretreatment and Enzymatic Hydrolysis of Wheat Straw for Enhancing Sugar Yields

Zhang,

2023

Fermentation

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Optimization of alkaline hydrogen peroxide (AHP) pretreatment and enzymatic hydrolysis of wheat straw (WS) was carried out to enhance fermentable sugar yields with the use of glucose and xylose yields from the pretreated WS as responses. In the first step, variables including temperature, hydrogen peroxide concentration and time during pretreatment were detected to have significant effects on the sugar yields. The results indicate that maximal sugar yields could be obtained while the WS was pretreated using 71 g/L hydrogen peroxide solution with 200 g/L of solid loading at 50 °C for 7.6 h. The corresponding cellulose recovery, hemicellulose recovery and lignin removal were 97.5%, 84.3% and 75.0%, respectively. In the second step, enzymatic hydrolysis of the pretreated WS was optimized. The results show that the reaction time, enzyme loading and biomass loading during enzymatic hydrolysis also had significant effects on the sugar yields. The final maximum yields of glucose (552.7 mg/gds (mg/g dry substrate)) and xylose (223.6 mg/gds) could be obtained while enzymatic hydrolysis was carried out at 50 °C for 37.0 h using 10.8 FPU/gds (filter paper activity unit per gram dry substrate) of enzyme loading, 88 g/L of biomass loading and 0.3% (w/v) of Tween-80. The corresponding cellulose conversion and hemicellulose conversion were 94.0% and 83.5%, respectively.

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“…The hydrolytic efficacy of crude enzyme was evaluated by enzymatic saccharification of alkali-treated wheat straw and rice straw and compared with the cellulases from Aspergillus niger ADH-11 and SIGMA. The commercial cellulase SIGMA and crude enzyme from Aspergillus niger ADH-11 was previously reported to be effective in saccharification of MAA treated wheat straw (Patel et al 2017 ). The time course profile of enzymatic saccharification using 5 FPU/g of enzyme dose is shown in Figure 7 .…”

Section: Resultsmentioning

confidence: 99%

“…Enzymatic saccharification of alkali-treated agro residues (wheat straw and rice straw) were carried out using 5 FPU/g of crude enzyme from Fomitopsis meliae CFA 2, 5 FPU/g of crude enzyme from Aspergillus niger ADH-11 and 5 FPU/g of commercial cellulase from SIGMA. Crude enzyme from Aspergillus niger ADH 11 was produced using previously optimised enzyme conditions under solid state fermentation (Patel et al 2017 ). Enzymatic saccharification was performed in 150-ml screw cap Erlenmeyer flasks containing 2.5% alkali-treated ago residues in 50 mM sodium citrate buffer (pH 4.8) containing 10 mg% sodium azide and 0.1% Tween-80 (v/v).…”

Section: Methodsmentioning

confidence: 99%

Fomitopsis meliae CFA 2, a novel brown rot for endoglucanase: emphasis towards enhanced endoglucanase production by statistical approach

2021

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Brown rot basidiomycetes are a principal group of wood-decaying fungi which degrade wood cellulose and hemicellulose by the combination of carbohydrate active enzymes and nonenzymatic oxidation reactions. Very scant information is available on carbohydrate active enzymes of brown rot fungi. In this context, present study focused on the production of cellulolytichemicellulolytic enzymes from newly isolated brown rot Fomitopsis meliae CFA 2. Under solidstate fermentation using wheat bran as the substrate Fomitopsis meliae CFA 2 was able to produce a maximum of 1391.12 ± 21.13 U/g of endoglucanase along with other cellulolytic and hemicellulolytic enzymes. Various fermentation parameters were optimised for enhanced production of endoglucanase by employing Plackett-Burman design followed by Box-Behnken design. A wellfitted regression equation with R 2 value of 98.91% was attained for endoglucanase. The yield of endoglucanase was enhanced by 1.83-fold after executing statistical optimisation of various fermentative parameters. The newly isolated Fomitopsis meliae CFA 2 was found to be a potential producer of endoglucanase. Enzymatic saccharification of alkali-treated wheat straw and rice straw resulted in release of 190.8 and 318.8 mg/g of reducing sugars, respectively.

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Microwave assisted alkali treated wheat straw as a substrate for co-production of (hemi)cellulolytic enzymes and development of balanced enzyme cocktail for its enhanced saccharification

Cited by 21 publications

References 37 publications

Optimization of potassium hydroxide combined urea pretreatment and enzymatic hydrolysis of wheat straw using response surface methodology for improving sugar production

Optimization of potassium hydroxide combined urea pretreatment and enzymatic hydrolysis of wheat straw using response surface methodology for improving sugar production

Optimization of Alkaline Hydrogen Peroxide Pretreatment and Enzymatic Hydrolysis of Wheat Straw for Enhancing Sugar Yields

Fomitopsis meliae CFA 2, a novel brown rot for endoglucanase: emphasis towards enhanced endoglucanase production by statistical approach

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