Microwave-assisted extraction in combination with capillary electrophoresis for rapid determination of isoquinoline alkaloids in Chelidonium majus L.

Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants

“…The contents of CHE and SAN in these Chinese herbal extracts are found to be 641.87 7.5 and 681.877.9 mg/kg for M. cordata, and 134.07 2.3 and 890.57 8.9 mg/kg for C. majus, respectively. To evaluate the accuracy of our method, the recovery was studied using a previously reported procedure [31]. Six replicate samples which were prepared by spiking known amounts of standards to a M. cordata sample were analyzed.…”

“…As an improvement, an ultra-performance liquid chromatography (UPLC) was developed [28], which showed some advantages such as reduced run time, increased peak capacities and less solvent consumption, but the technique still requires tedious sample preparation (reflux extraction followed by solid-phase extraction) prior to UPLC analysis. Conventional CE methods were also reported for the analysis of CHE and SAN [16,[29][30][31]. As these compounds are insoluble in water, organic solutions were used as the run buffers [16], or used as buffer additives [31].…”

“…Conventional CE methods were also reported for the analysis of CHE and SAN [16,[29][30][31]. As these compounds are insoluble in water, organic solutions were used as the run buffers [16], or used as buffer additives [31].…”

Microwave‐assisted extraction in combination with HPLC‐UV for quantitative analysis of six bioactive oxoisoaporphine alkaloids in Menispermum dauricum DC

“…Nowadays, microwave-assisted extraction (MAE) has become one of the most powerful sample extraction techniques. With the advantages of high reproducibility, time savings and less solvent consumption, MAE has been successfully applied to the extraction of some bioactive constituents in TCMs (Zhou et al, 2012;Tan et al, 2011). In this study, a simple and rapid method based on MAE followed by HPLC-UV is proposed for the first time for the simultaneous quantification of six OA in MDD, including bianfugedine (1), menisporphine (2), 6-O-demethylmenisporphine (3), bianfugecine (4), dauriporphine (5) and dauriporphinoline (6) (Fig.…”

Comparison of microwave‐assisted and conventional extraction of mangiferin from mango (Mangifera indicaL.) leaves

“…Among these, MAE is a rapid and efficient extraction technique . It can utilize the energy of microwaves to cause molecular movement with a permanent dipole, lead to a fast heating of the solvent and sample, and offer advantages such as reduced extraction time and improved efficiency compared with other extraction methods . Therefore, MAE has been widely applied to the extraction of many natural products .…”