Microwave-Assisted Extraction of Inulin From Jerusalem Artichoke and Partial Acid Hydrolyses

Abstract

The Recent study aimed to identify the optimum conditions for inulin extraction from jerusalem artichoke tuber (JAT) powder using microwave assisted extraction method, and partially hydrolysis of purified inulin for producing fructooligosaccharide (FOS) using citric acid & microwave energy. The extraction conducted at different temperature (90 – 95) C˚ at (700 W/5min, 450w/8 min and 350w/14 min). The extracted inulin was concentrated to (50 – 60) % of original volume using rotary evaporator, and purified by lime method. The purified inulin extract was concentrated to 32 brix and mixed with acetone (3:1 acetone : extract), kept for 24 h at 4 C˚, then centrifuged at 10000g / 15 min., the precipitate dried at 55 ˚C. Acid hydrolysis of purified inulin carried out at different pH values (1.5, 2.5 & 3.0) at (90 ±2) ˚C. Aliquot of inulin hydrolysate were taken after (5, 10, 15, 20, 25 & 30) min. and subjected to qualitative analysis by RP-HPLC and TLC. The obtained results indicated that the microwave assisted extraction at 700 W/ 5 min. / 95 ˚C was superior as compared to the rest. The yield of extracted inulin was about 39.61 % and the extraction efficiency was 94.31 %, while the optimum condition for inulin acid hydrolysis appeared to be at 90 ˚C/ 15 min at pH 2.5 and 450 W. The qualitative analysis using (RP-HPLC & TLC) showed that the DP of inulin units ranged from (2 - 35) and for FOS ranged from (2 – 9) unit.

تقنية كرموتوغرافيا السائل ذات الاداء العالي ذوالطور العكسي مع كاشف الاشعة الفوق البنفسجية لتقدير ادوية الايبو بروفين والتنوكسيكام في الصورة النقية و الاقراص الدوائية

“…TNX obstructs the actions of COX enzymes, the chemicals responsible for pain and inflammation. Various techniques have been employed to determine the levels of TNX, including HPLC 15 , UV 16 , fluorescence 17 , flow injection 18 , and ionpair 19 spectrophotometry, electro analytical 20 , voltammetry 21 , X-ray 22 , scanning electron microscopy (SEM), X-ray diffraction, dynamic light scattering (DLS), Fourier transform infrared (FTIR) 23 , photo catalysis 24 , and immunochromatographic strip with colloidal gold 25 .…”