Microwave effects on acetylcholine‐induced channels in cultured chick myotubes

Abstract

The behavior of cultured myotubes from chick embryos exposed to microwaves has been experimentally analyzed. Recordings of acetylcholine-induced currents have been obtained via patch-clamp techniques using both cell-attached (single-channel current recording) and whole-cell (total current recording) configurations. During the exposure to low-power microwaves the frequency of the ACh-activated single channel openings decreased, while the ACh-induced total current showed a faster falling phase. Channel open time and conductance were not affected by microwave irradiation. It is concluded that the exposure to microwaves increases the rate of desensitization and decreases the channel opening probability. The nonthermal origin and the molecular interaction mechanisms governing these electromagnetic-induced effects are discussed.

Dual effects of microwaves on single Ca2+‐activated K+ channels in cultured kidney cells Vero

“…However, we reject this mechamsm primarily due to (i) the opposite effects of the field on the channels with high and low affinity for Ca 2+ and (ii) the absence of any effect of the field on the unit channel conductance y. There are also other reasons why this mechanism is unacceptable [3,10,18].…”

“…A horizontal line indicates the expected behaviour of the relationship in the absence of the EMF effect on n. As can be seen, ifn ° is, on average, more than 1, the field reduces it; conversely, if n ° is less than 1, the field would increase it. Thus, the degree and direction of the EMF effects on the parameters of the Kca channels depend on their initial biological status (see also [1,6,18]). What is a possible mechanism of the observed effects of the microwaves on the Kca channels?…”

“…Under these conditions the estimated power density of the EMF in the vicinity of the membrane patch was about 100/IW/cm 2. In the estimations the data and suppositions of Gandi [17] and Inzeo et al [18] were used. The experiments were carried out at a stabilized temperature of 22°C: without stabilization the temperature of the resting bath solution was raised no more than 0.5°C due to the microwave irradiation.…”

Immunomodulating action of low intensity millimeter waves on primed neutrophils

“…But opsonized zymosan is very complex stimulus to analyse the intracellular mechanisms. Nonthermal effects of continuous mm waves were revealed in studies of various membrane functions [D'Inzeo et al, 1988;Kataev et al, 1993;Geletyuk et al, 1995;Pakhomov et al, 1997]. In contrast, it was shown that a thermal mechanism underlies the effects of mm waves on ionic currents of Lymnaea neurons [Alekseev and Ziskin, 1999].…”

Effect of Chronic Intermittent Exposure to AM Radiofrequency Field on Responses to Various Types of Noxious Stimuli in Growing Rats

“…During the last decade, several reports have supported the concept that the cell membranes of the brain are critical structures perceiving the action of microwaves (D'Inzeo et al, 1988;Adey, 1993), which is reflected in the underlying neurochemical profile (Merrit et al, 1976;Kunjilwar and Behari, 1993;Lai, 1994;Yurinskaya et al, 1996) and the electrophysiological events (Papi et al, 1995). Both the neural and neurochemical alterations by RF field may contribute towards the aberrant behavior including nociceptive behavior (Behari and Paul Raj, 2007).…”